VvWRKY13 enhances ABA biosynthesis in Vitis vinifera
Abscisic acid (ABA) plays critical roles in plant growth and development as well as in plants’ responses to abiotic stresses. We previously isolated VvWRKY13, a novel transcription factor, from Vitis vinifera (grapevine), and here we present evidence that VvWRKY13 may regulate ABA biosynthesis in pl...
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| Format: | Article |
| Language: | English |
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Polish Botanical Society
2017-06-01
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| Series: | Acta Societatis Botanicorum Poloniae |
| Subjects: | |
| Online Access: | https://pbsociety.org.pl/journals/index.php/asbp/article/view/6752 |
| _version_ | 1818553610461511680 |
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| author | JIe Hao Qian Ma Lixia Hou Fanggui Zhao Liu Xin |
| author_facet | JIe Hao Qian Ma Lixia Hou Fanggui Zhao Liu Xin |
| author_sort | JIe Hao |
| collection | DOAJ |
| description | Abscisic acid (ABA) plays critical roles in plant growth and development as well as in plants’ responses to abiotic stresses. We previously isolated VvWRKY13, a novel transcription factor, from Vitis vinifera (grapevine), and here we present evidence that VvWRKY13 may regulate ABA biosynthesis in plants. When VvWRKY13 was ectopically expressed in Arabidopsis, the transgenic lines showed delayed seed germination, smaller stomatal aperture size, and several other phenotypic changes, indicating elevated ABA levels in these plants. Sequence analysis of several genes that are involved in grapevine ABA synthetic pathway identified WRKY-specific binding elements (W-box or W-like box) in the promoter regions. Indeed, transient overexpression of VvWRKY13 in grapevine leaves significantly increased the transcript levels of ABA synthetic pathway genes. Taken together, we conclude that VvWRKY13 may promote ABA production by activating genes in the ABA synthetic pathway. |
| first_indexed | 2024-12-12T09:28:05Z |
| format | Article |
| id | doaj.art-464ce77c60f044a89561d53e6061e9b9 |
| institution | Directory Open Access Journal |
| issn | 2083-9480 |
| language | English |
| last_indexed | 2024-12-12T09:28:05Z |
| publishDate | 2017-06-01 |
| publisher | Polish Botanical Society |
| record_format | Article |
| series | Acta Societatis Botanicorum Poloniae |
| spelling | doaj.art-464ce77c60f044a89561d53e6061e9b92022-12-22T00:28:57ZengPolish Botanical SocietyActa Societatis Botanicorum Poloniae2083-94802017-06-0186210.5586/asbp.35466805VvWRKY13 enhances ABA biosynthesis in Vitis viniferaJIe Hao0Qian Ma1Lixia Hou2Fanggui Zhao3Liu Xin4Shandong Key Laboratory of Plant Biotechnology, Qingdao Key Laboratory of Genetic Improvement and Breeding in Horticultural Plants, College of Life Sciences, Qingdao Agricultural University, Qingdao 266109Shandong Key Laboratory of Plant Biotechnology, Qingdao Key Laboratory of Genetic Improvement and Breeding in Horticultural Plants, College of Life Sciences, Qingdao Agricultural University, Qingdao 266109Shandong Key Laboratory of Plant Biotechnology, Qingdao Key Laboratory of Genetic Improvement and Breeding in Horticultural Plants, College of Life Sciences, Qingdao Agricultural University, Qingdao 266109Shandong Key Laboratory of Plant Biotechnology, Qingdao Key Laboratory of Genetic Improvement and Breeding in Horticultural Plants, College of Life Sciences, Qingdao Agricultural University, Qingdao 266109Shandong Key Laboratory of Plant Biotechnology, Qingdao Key Laboratory of Genetic Improvement and Breeding in Horticultural Plants, College of Life Sciences, Qingdao Agricultural University, Qingdao 266109Abscisic acid (ABA) plays critical roles in plant growth and development as well as in plants’ responses to abiotic stresses. We previously isolated VvWRKY13, a novel transcription factor, from Vitis vinifera (grapevine), and here we present evidence that VvWRKY13 may regulate ABA biosynthesis in plants. When VvWRKY13 was ectopically expressed in Arabidopsis, the transgenic lines showed delayed seed germination, smaller stomatal aperture size, and several other phenotypic changes, indicating elevated ABA levels in these plants. Sequence analysis of several genes that are involved in grapevine ABA synthetic pathway identified WRKY-specific binding elements (W-box or W-like box) in the promoter regions. Indeed, transient overexpression of VvWRKY13 in grapevine leaves significantly increased the transcript levels of ABA synthetic pathway genes. Taken together, we conclude that VvWRKY13 may promote ABA production by activating genes in the ABA synthetic pathway.https://pbsociety.org.pl/journals/index.php/asbp/article/view/6752VvWRKY13transcription factorsenescenceABAVitis vinifera |
| spellingShingle | JIe Hao Qian Ma Lixia Hou Fanggui Zhao Liu Xin VvWRKY13 enhances ABA biosynthesis in Vitis vinifera Acta Societatis Botanicorum Poloniae VvWRKY13 transcription factor senescence ABA Vitis vinifera |
| title | VvWRKY13 enhances ABA biosynthesis in Vitis vinifera |
| title_full | VvWRKY13 enhances ABA biosynthesis in Vitis vinifera |
| title_fullStr | VvWRKY13 enhances ABA biosynthesis in Vitis vinifera |
| title_full_unstemmed | VvWRKY13 enhances ABA biosynthesis in Vitis vinifera |
| title_short | VvWRKY13 enhances ABA biosynthesis in Vitis vinifera |
| title_sort | vvwrky13 enhances aba biosynthesis in vitis vinifera |
| topic | VvWRKY13 transcription factor senescence ABA Vitis vinifera |
| url | https://pbsociety.org.pl/journals/index.php/asbp/article/view/6752 |
| work_keys_str_mv | AT jiehao vvwrky13enhancesababiosynthesisinvitisvinifera AT qianma vvwrky13enhancesababiosynthesisinvitisvinifera AT lixiahou vvwrky13enhancesababiosynthesisinvitisvinifera AT fangguizhao vvwrky13enhancesababiosynthesisinvitisvinifera AT liuxin vvwrky13enhancesababiosynthesisinvitisvinifera |