BDNF Regulates the Expression of Fragile X Mental Retardation Protein mRNA in the Hippocampus
Both fragile X mental retardation protein (FMRP) and brain-derived neurotrophic factor (BDNF) are implicated in the maturation of neurons and in the higher cognitive functions. We have investigated whether FMRP and BDNF are reciprocally regulated in neurons. Exposure of cultured hippocampal neurons...
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| Format: | Article |
| Language: | English |
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Elsevier
2002-10-01
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| Series: | Neurobiology of Disease |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0969996102905449 |
| _version_ | 1818610243665395712 |
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| author | Maija Castrén Katariina E Lampinen Riitta Miettinen Eija Koponen Ilkka Sipola Cathy E Bakker Ben A Oostra Eero Castrén |
| author_facet | Maija Castrén Katariina E Lampinen Riitta Miettinen Eija Koponen Ilkka Sipola Cathy E Bakker Ben A Oostra Eero Castrén |
| author_sort | Maija Castrén |
| collection | DOAJ |
| description | Both fragile X mental retardation protein (FMRP) and brain-derived neurotrophic factor (BDNF) are implicated in the maturation of neurons and in the higher cognitive functions. We have investigated whether FMRP and BDNF are reciprocally regulated in neurons. Exposure of cultured hippocampal neurons to BDNF, but not to NT-3, reduced FMR1 mRNA levels to 84.8% of control at 4 h and the levels were back to baseline by 24 h or 4 days. Furthermore, expression of FMR1 mRNA was reduced (82.4% of control) in vivo in the hippocampus of transgenic mice overexpressing TrkB receptors, and a small but significant (5.1%) decrease was also detected in FMRP protein levels. In contrast, the expression patterns of BDNF and TrkB mRNAs were not altered in FMRP-deficient mice compared to wild-type mice. Our data provide evidence that BDNF via TrkB signaling decreases FMRP expression and suggest a role for FMRP in BDNF-induced synaptic plasticity. |
| first_indexed | 2024-12-16T15:11:20Z |
| format | Article |
| id | doaj.art-464ffc8ecfda4074b8d09e61e0b47f70 |
| institution | Directory Open Access Journal |
| issn | 1095-953X |
| language | English |
| last_indexed | 2024-12-16T15:11:20Z |
| publishDate | 2002-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Neurobiology of Disease |
| spelling | doaj.art-464ffc8ecfda4074b8d09e61e0b47f702022-12-21T22:26:56ZengElsevierNeurobiology of Disease1095-953X2002-10-01111221229BDNF Regulates the Expression of Fragile X Mental Retardation Protein mRNA in the HippocampusMaija Castrén0Katariina E Lampinen1Riitta Miettinen2Eija Koponen3Ilkka Sipola4Cathy E Bakker5Ben A Oostra6Eero Castrén7Department of Neurobiology, A. I. Virtanen Institute, University of Kuopio, FIN-70210, Kuopio, Finland; Department of Neurology, University of Kuopio, FIN-70210, Kuopio, Finland; Department of Psychiatry, University of Kuopio, FIN-70210, Kuopio, Finland; Department of Clinical Genetics, Erasmus University, Rotterdam, The NetherlandsDepartment of Neurobiology, A. I. Virtanen Institute, University of Kuopio, FIN-70210, Kuopio, Finland; Department of Neurology, University of Kuopio, FIN-70210, Kuopio, Finland; Department of Psychiatry, University of Kuopio, FIN-70210, Kuopio, Finland; Department of Clinical Genetics, Erasmus University, Rotterdam, The NetherlandsDepartment of Neurobiology, A. I. Virtanen Institute, University of Kuopio, FIN-70210, Kuopio, Finland; Department of Neurology, University of Kuopio, FIN-70210, Kuopio, Finland; Department of Psychiatry, University of Kuopio, FIN-70210, Kuopio, Finland; Department of Clinical Genetics, Erasmus University, Rotterdam, The NetherlandsDepartment of Neurobiology, A. I. Virtanen Institute, University of Kuopio, FIN-70210, Kuopio, Finland; Department of Neurology, University of Kuopio, FIN-70210, Kuopio, Finland; Department of Psychiatry, University of Kuopio, FIN-70210, Kuopio, Finland; Department of Clinical Genetics, Erasmus University, Rotterdam, The NetherlandsDepartment of Neurobiology, A. I. Virtanen Institute, University of Kuopio, FIN-70210, Kuopio, Finland; Department of Neurology, University of Kuopio, FIN-70210, Kuopio, Finland; Department of Psychiatry, University of Kuopio, FIN-70210, Kuopio, Finland; Department of Clinical Genetics, Erasmus University, Rotterdam, The NetherlandsDepartment of Neurobiology, A. I. Virtanen Institute, University of Kuopio, FIN-70210, Kuopio, Finland; Department of Neurology, University of Kuopio, FIN-70210, Kuopio, Finland; Department of Psychiatry, University of Kuopio, FIN-70210, Kuopio, Finland; Department of Clinical Genetics, Erasmus University, Rotterdam, The NetherlandsDepartment of Neurobiology, A. I. Virtanen Institute, University of Kuopio, FIN-70210, Kuopio, Finland; Department of Neurology, University of Kuopio, FIN-70210, Kuopio, Finland; Department of Psychiatry, University of Kuopio, FIN-70210, Kuopio, Finland; Department of Clinical Genetics, Erasmus University, Rotterdam, The NetherlandsDepartment of Neurobiology, A. I. Virtanen Institute, University of Kuopio, FIN-70210, Kuopio, Finland; Department of Neurology, University of Kuopio, FIN-70210, Kuopio, Finland; Department of Psychiatry, University of Kuopio, FIN-70210, Kuopio, Finland; Department of Clinical Genetics, Erasmus University, Rotterdam, The NetherlandsBoth fragile X mental retardation protein (FMRP) and brain-derived neurotrophic factor (BDNF) are implicated in the maturation of neurons and in the higher cognitive functions. We have investigated whether FMRP and BDNF are reciprocally regulated in neurons. Exposure of cultured hippocampal neurons to BDNF, but not to NT-3, reduced FMR1 mRNA levels to 84.8% of control at 4 h and the levels were back to baseline by 24 h or 4 days. Furthermore, expression of FMR1 mRNA was reduced (82.4% of control) in vivo in the hippocampus of transgenic mice overexpressing TrkB receptors, and a small but significant (5.1%) decrease was also detected in FMRP protein levels. In contrast, the expression patterns of BDNF and TrkB mRNAs were not altered in FMRP-deficient mice compared to wild-type mice. Our data provide evidence that BDNF via TrkB signaling decreases FMRP expression and suggest a role for FMRP in BDNF-induced synaptic plasticity.http://www.sciencedirect.com/science/article/pii/S0969996102905449fragile X syndromeFmr1brain-derived neurotrophic factorTrkBgene expression |
| spellingShingle | Maija Castrén Katariina E Lampinen Riitta Miettinen Eija Koponen Ilkka Sipola Cathy E Bakker Ben A Oostra Eero Castrén BDNF Regulates the Expression of Fragile X Mental Retardation Protein mRNA in the Hippocampus Neurobiology of Disease fragile X syndrome Fmr1 brain-derived neurotrophic factor TrkB gene expression |
| title | BDNF Regulates the Expression of Fragile X Mental Retardation Protein mRNA in the Hippocampus |
| title_full | BDNF Regulates the Expression of Fragile X Mental Retardation Protein mRNA in the Hippocampus |
| title_fullStr | BDNF Regulates the Expression of Fragile X Mental Retardation Protein mRNA in the Hippocampus |
| title_full_unstemmed | BDNF Regulates the Expression of Fragile X Mental Retardation Protein mRNA in the Hippocampus |
| title_short | BDNF Regulates the Expression of Fragile X Mental Retardation Protein mRNA in the Hippocampus |
| title_sort | bdnf regulates the expression of fragile x mental retardation protein mrna in the hippocampus |
| topic | fragile X syndrome Fmr1 brain-derived neurotrophic factor TrkB gene expression |
| url | http://www.sciencedirect.com/science/article/pii/S0969996102905449 |
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