Construction of an SSR and RAD-Marker Based Molecular Linkage Map of Vigna vexillata (L.) A. Rich.
Vigna vexillata (L.) A. Rich. (tuber cowpea) is an underutilized crop for consuming its tuber and mature seeds. Wild form of V. vexillata is a pan-tropical perennial herbaceous plant which has been used by local people as a food. Wild V. vexillata has also been considered as useful gene(s) source fo...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4580594?pdf=render |
| _version_ | 1819065191043694592 |
|---|---|
| author | Rusama Marubodee Eri Ogiso-Tanaka Takehisa Isemura Sompong Chankaew Akito Kaga Ken Naito Hiroshi Ehara Norihiko Tomooka |
| author_facet | Rusama Marubodee Eri Ogiso-Tanaka Takehisa Isemura Sompong Chankaew Akito Kaga Ken Naito Hiroshi Ehara Norihiko Tomooka |
| author_sort | Rusama Marubodee |
| collection | DOAJ |
| description | Vigna vexillata (L.) A. Rich. (tuber cowpea) is an underutilized crop for consuming its tuber and mature seeds. Wild form of V. vexillata is a pan-tropical perennial herbaceous plant which has been used by local people as a food. Wild V. vexillata has also been considered as useful gene(s) source for V. unguiculata (cowpea), since it was reported to have various resistance gene(s) for insects and diseases of cowpea. To exploit the potential of V. vexillata, an SSR-based linkage map of V. vexillata was developed. A total of 874 SSR markers successfully amplified single DNA fragment in V. vexillata among 1,336 SSR markers developed from Vigna angularis (azuki bean), V. unguiculata and Phaseolus vulgaris (common bean). An F2 population of 300 plants derived from a cross between salt resistant (V1) and susceptible (V5) accessions was used for mapping. A genetic linkage map was constructed using 82 polymorphic SSR markers loci, which could be assigned to 11 linkage groups spanning 511.5 cM in length with a mean distance of 7.2 cM between adjacent markers. To develop higher density molecular linkage map and to confirm SSR markers position in a linkage map, RAD markers were developed and a combined SSR and RAD markers linkage map of V. vexillata was constructed. A total of 559 (84 SSR and 475 RAD) markers loci could be assigned to 11 linkage groups spanning 973.9 cM in length with a mean distance of 1.8 cM between adjacent markers. Linkage and genetic position of all SSR markers in an SSR linkage map were confirmed. When an SSR genetic linkage map of V. vexillata was compared with those of V. radiata and V. unguiculata, it was suggested that the structure of V. vexillata chromosome was considerably differentiated. This map is the first SSR and RAD marker-based V. vexillata linkage map which can be used for the mapping of useful traits. |
| first_indexed | 2024-12-21T15:42:32Z |
| format | Article |
| id | doaj.art-4650f628ef2a48cb802aa237e4b436c8 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T15:42:32Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-4650f628ef2a48cb802aa237e4b436c82022-12-21T18:58:27ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01109e013894210.1371/journal.pone.0138942Construction of an SSR and RAD-Marker Based Molecular Linkage Map of Vigna vexillata (L.) A. Rich.Rusama MarubodeeEri Ogiso-TanakaTakehisa IsemuraSompong ChankaewAkito KagaKen NaitoHiroshi EharaNorihiko TomookaVigna vexillata (L.) A. Rich. (tuber cowpea) is an underutilized crop for consuming its tuber and mature seeds. Wild form of V. vexillata is a pan-tropical perennial herbaceous plant which has been used by local people as a food. Wild V. vexillata has also been considered as useful gene(s) source for V. unguiculata (cowpea), since it was reported to have various resistance gene(s) for insects and diseases of cowpea. To exploit the potential of V. vexillata, an SSR-based linkage map of V. vexillata was developed. A total of 874 SSR markers successfully amplified single DNA fragment in V. vexillata among 1,336 SSR markers developed from Vigna angularis (azuki bean), V. unguiculata and Phaseolus vulgaris (common bean). An F2 population of 300 plants derived from a cross between salt resistant (V1) and susceptible (V5) accessions was used for mapping. A genetic linkage map was constructed using 82 polymorphic SSR markers loci, which could be assigned to 11 linkage groups spanning 511.5 cM in length with a mean distance of 7.2 cM between adjacent markers. To develop higher density molecular linkage map and to confirm SSR markers position in a linkage map, RAD markers were developed and a combined SSR and RAD markers linkage map of V. vexillata was constructed. A total of 559 (84 SSR and 475 RAD) markers loci could be assigned to 11 linkage groups spanning 973.9 cM in length with a mean distance of 1.8 cM between adjacent markers. Linkage and genetic position of all SSR markers in an SSR linkage map were confirmed. When an SSR genetic linkage map of V. vexillata was compared with those of V. radiata and V. unguiculata, it was suggested that the structure of V. vexillata chromosome was considerably differentiated. This map is the first SSR and RAD marker-based V. vexillata linkage map which can be used for the mapping of useful traits.http://europepmc.org/articles/PMC4580594?pdf=render |
| spellingShingle | Rusama Marubodee Eri Ogiso-Tanaka Takehisa Isemura Sompong Chankaew Akito Kaga Ken Naito Hiroshi Ehara Norihiko Tomooka Construction of an SSR and RAD-Marker Based Molecular Linkage Map of Vigna vexillata (L.) A. Rich. PLoS ONE |
| title | Construction of an SSR and RAD-Marker Based Molecular Linkage Map of Vigna vexillata (L.) A. Rich. |
| title_full | Construction of an SSR and RAD-Marker Based Molecular Linkage Map of Vigna vexillata (L.) A. Rich. |
| title_fullStr | Construction of an SSR and RAD-Marker Based Molecular Linkage Map of Vigna vexillata (L.) A. Rich. |
| title_full_unstemmed | Construction of an SSR and RAD-Marker Based Molecular Linkage Map of Vigna vexillata (L.) A. Rich. |
| title_short | Construction of an SSR and RAD-Marker Based Molecular Linkage Map of Vigna vexillata (L.) A. Rich. |
| title_sort | construction of an ssr and rad marker based molecular linkage map of vigna vexillata l a rich |
| url | http://europepmc.org/articles/PMC4580594?pdf=render |
| work_keys_str_mv | AT rusamamarubodee constructionofanssrandradmarkerbasedmolecularlinkagemapofvignavexillatalarich AT eriogisotanaka constructionofanssrandradmarkerbasedmolecularlinkagemapofvignavexillatalarich AT takehisaisemura constructionofanssrandradmarkerbasedmolecularlinkagemapofvignavexillatalarich AT sompongchankaew constructionofanssrandradmarkerbasedmolecularlinkagemapofvignavexillatalarich AT akitokaga constructionofanssrandradmarkerbasedmolecularlinkagemapofvignavexillatalarich AT kennaito constructionofanssrandradmarkerbasedmolecularlinkagemapofvignavexillatalarich AT hiroshiehara constructionofanssrandradmarkerbasedmolecularlinkagemapofvignavexillatalarich AT norihikotomooka constructionofanssrandradmarkerbasedmolecularlinkagemapofvignavexillatalarich |