Description of Hutmannin-1, a new piii-metalloprotease from the venom of the neotropical lansberg’s hognose Viper (Porthidium lansbergii hutmanni) With fibrino(geno) lytic and haemorrhagic activities
The objective was to characterise hutmannin-1 (hut-1), a new~62 kDa P-III-class metalloprotease from Porthidium lansbergii hutmanni(P.l.h) (Margarita Island, Venezuela). To characterise this protein, the crude venom of P.l.h was fractionated by size exclusion chromatography, anion exchange chromat...
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Universidad del Zulia
2021-04-01
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| Series: | Revista Científica |
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| Online Access: | http://www.produccioncientificaluz.org/index.php/cientifica/article/view/35747 |
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| author | María Eugenia Pineda Jonás Perales Elda Eliza Sánchez Tomas Hermoso María Narvaez Alexander Chapeaurouge Alba Marlene Vargas Alexis Rodríguez-Acosta |
| author_facet | María Eugenia Pineda Jonás Perales Elda Eliza Sánchez Tomas Hermoso María Narvaez Alexander Chapeaurouge Alba Marlene Vargas Alexis Rodríguez-Acosta |
| author_sort | María Eugenia Pineda |
| collection | DOAJ |
| description |
The objective was to characterise hutmannin-1 (hut-1), a new~62 kDa P-III-class metalloprotease from Porthidium lansbergii hutmanni(P.l.h) (Margarita Island, Venezuela). To characterise this protein, the crude venom of P.l.h was fractionated by size exclusion chromatography, anion exchange chromatography and High Performance Liquid Chromatography (HPLC). Hutmannin-1 was identified by MALDI-TOF/TOF mass spectrometry, and the venom was analysed by SDS-PAGE. The lethality, minimum haemorrhagic dose (MHD), effect of temperature on the activity, procoagulant activity on human plasma, and anticoagulant, defibrinating, gelatinolytic fibrinolytic, and fibrinogenolytic and platelet aggregation activities of hut-1 were determined. Antigenic recognition assays were performed on P.l.h crude venom and hut-1 by a venezuelan polyvalent anti-ophidic serum (PAOS) Hut-1 had strong fibrinogenolytic and moderate fibrinolytic activity. These activities and the haemorrhagic activity of hut-1 were completely inhibited by EDTA. P.l.h crude venom had potent anticoagulant activity on recalcified plasma and inhibited the platelet aggregation induced by thrombin, ADP, collagen and ristocetin. In contrast, the anticoagulant, coagulant and platelet aggregation inhibition of hut-1 were not observed with any of the agonists. This result suggests that other proteins in the crude venom, markedly impact platelet functions and/or coagulation factors. Commercial venezuelan antivenin showed limited ability to neutralise the haemorrhagic activity of hut-1.
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| first_indexed | 2024-04-10T06:43:48Z |
| format | Article |
| id | doaj.art-46518e199ff54d42abfc5b76eb45fb96 |
| institution | Directory Open Access Journal |
| issn | 0798-2259 2521-9715 |
| language | English |
| last_indexed | 2024-04-10T06:43:48Z |
| publishDate | 2021-04-01 |
| publisher | Universidad del Zulia |
| record_format | Article |
| series | Revista Científica |
| spelling | doaj.art-46518e199ff54d42abfc5b76eb45fb962023-02-28T15:18:45ZengUniversidad del ZuliaRevista Científica0798-22592521-97152021-04-01303Description of Hutmannin-1, a new piii-metalloprotease from the venom of the neotropical lansberg’s hognose Viper (Porthidium lansbergii hutmanni) With fibrino(geno) lytic and haemorrhagic activitiesMaría Eugenia Pineda0Jonás Perales1Elda Eliza Sánchez2Tomas Hermoso3María Narvaez4Alexander Chapeaurouge5Alba Marlene Vargas6Alexis Rodríguez-Acosta7Universidad Central de VenezuelaLaboratory of Toxinology, Oswaldo Cruz InstituteTexas A&M University-KingsvilleUniversidad Central de VenezuelaUniversidad Central de VenezuelaLaboratory of Toxinology, Oswaldo Cruz InstituteUniversidad Central de VenezuelaUniversidad Central de Venezuela The objective was to characterise hutmannin-1 (hut-1), a new~62 kDa P-III-class metalloprotease from Porthidium lansbergii hutmanni(P.l.h) (Margarita Island, Venezuela). To characterise this protein, the crude venom of P.l.h was fractionated by size exclusion chromatography, anion exchange chromatography and High Performance Liquid Chromatography (HPLC). Hutmannin-1 was identified by MALDI-TOF/TOF mass spectrometry, and the venom was analysed by SDS-PAGE. The lethality, minimum haemorrhagic dose (MHD), effect of temperature on the activity, procoagulant activity on human plasma, and anticoagulant, defibrinating, gelatinolytic fibrinolytic, and fibrinogenolytic and platelet aggregation activities of hut-1 were determined. Antigenic recognition assays were performed on P.l.h crude venom and hut-1 by a venezuelan polyvalent anti-ophidic serum (PAOS) Hut-1 had strong fibrinogenolytic and moderate fibrinolytic activity. These activities and the haemorrhagic activity of hut-1 were completely inhibited by EDTA. P.l.h crude venom had potent anticoagulant activity on recalcified plasma and inhibited the platelet aggregation induced by thrombin, ADP, collagen and ristocetin. In contrast, the anticoagulant, coagulant and platelet aggregation inhibition of hut-1 were not observed with any of the agonists. This result suggests that other proteins in the crude venom, markedly impact platelet functions and/or coagulation factors. Commercial venezuelan antivenin showed limited ability to neutralise the haemorrhagic activity of hut-1. http://www.produccioncientificaluz.org/index.php/cientifica/article/view/35747Anticoagulantantivenincoagulationhaemostasishaemorrhagesnake venom |
| spellingShingle | María Eugenia Pineda Jonás Perales Elda Eliza Sánchez Tomas Hermoso María Narvaez Alexander Chapeaurouge Alba Marlene Vargas Alexis Rodríguez-Acosta Description of Hutmannin-1, a new piii-metalloprotease from the venom of the neotropical lansberg’s hognose Viper (Porthidium lansbergii hutmanni) With fibrino(geno) lytic and haemorrhagic activities Revista Científica Anticoagulant antivenin coagulation haemostasis haemorrhage snake venom |
| title | Description of Hutmannin-1, a new piii-metalloprotease from the venom of the neotropical lansberg’s hognose Viper (Porthidium lansbergii hutmanni) With fibrino(geno) lytic and haemorrhagic activities |
| title_full | Description of Hutmannin-1, a new piii-metalloprotease from the venom of the neotropical lansberg’s hognose Viper (Porthidium lansbergii hutmanni) With fibrino(geno) lytic and haemorrhagic activities |
| title_fullStr | Description of Hutmannin-1, a new piii-metalloprotease from the venom of the neotropical lansberg’s hognose Viper (Porthidium lansbergii hutmanni) With fibrino(geno) lytic and haemorrhagic activities |
| title_full_unstemmed | Description of Hutmannin-1, a new piii-metalloprotease from the venom of the neotropical lansberg’s hognose Viper (Porthidium lansbergii hutmanni) With fibrino(geno) lytic and haemorrhagic activities |
| title_short | Description of Hutmannin-1, a new piii-metalloprotease from the venom of the neotropical lansberg’s hognose Viper (Porthidium lansbergii hutmanni) With fibrino(geno) lytic and haemorrhagic activities |
| title_sort | description of hutmannin 1 a new piii metalloprotease from the venom of the neotropical lansberg s hognose viper porthidium lansbergii hutmanni with fibrino geno lytic and haemorrhagic activities |
| topic | Anticoagulant antivenin coagulation haemostasis haemorrhage snake venom |
| url | http://www.produccioncientificaluz.org/index.php/cientifica/article/view/35747 |
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