Expression of uncharacterized male germ cell-specific genes and discovery of novel sperm-tail proteins in mice.
The identification and characterization of germ cell-specific genes are essential if we hope to comprehensively understand the mechanisms of spermatogenesis and fertilization. Here, we searched the mouse UniGene databases and identified 13 novel genes as being putatively testis-specific or -predomin...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5526581?pdf=render |
| _version_ | 1818839470698397696 |
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| author | Jun Tae Kwon Sera Ham Suyeon Jeon Youil Kim Seungmin Oh Chunghee Cho |
| author_facet | Jun Tae Kwon Sera Ham Suyeon Jeon Youil Kim Seungmin Oh Chunghee Cho |
| author_sort | Jun Tae Kwon |
| collection | DOAJ |
| description | The identification and characterization of germ cell-specific genes are essential if we hope to comprehensively understand the mechanisms of spermatogenesis and fertilization. Here, we searched the mouse UniGene databases and identified 13 novel genes as being putatively testis-specific or -predominant. Our in silico and in vitro analyses revealed that the expressions of these genes are testis- and germ cell-specific, and that they are regulated in a stage-specific manner during spermatogenesis. We generated antibodies against the proteins encoded by seven of the genes to facilitate their characterization in male germ cells. Immunoblotting and immunofluorescence analyses revealed that one of these proteins was expressed only in testicular germ cells, three were expressed in both testicular germ cells and testicular sperm, and the remaining three were expressed in sperm of the testicular stages and in mature sperm from the epididymis. Further analysis of the latter three proteins showed that they were all associated with cytoskeletal structures in the sperm flagellum. Among them, MORN5, which is predicted to contain three MORN motifs, is conserved between mouse and human sperm. In conclusion, we herein identify 13 authentic genes with male germ cell-specific expression, and provide comprehensive information about these genes and their encoded products. Our finding will facilitate future investigations into the functional roles of these novel genes in spermatogenesis and sperm functions. |
| first_indexed | 2024-12-19T03:54:48Z |
| format | Article |
| id | doaj.art-465271fa38ba4cf0861027fea60db736 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-19T03:54:48Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-465271fa38ba4cf0861027fea60db7362022-12-21T20:36:51ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01127e018203810.1371/journal.pone.0182038Expression of uncharacterized male germ cell-specific genes and discovery of novel sperm-tail proteins in mice.Jun Tae KwonSera HamSuyeon JeonYouil KimSeungmin OhChunghee ChoThe identification and characterization of germ cell-specific genes are essential if we hope to comprehensively understand the mechanisms of spermatogenesis and fertilization. Here, we searched the mouse UniGene databases and identified 13 novel genes as being putatively testis-specific or -predominant. Our in silico and in vitro analyses revealed that the expressions of these genes are testis- and germ cell-specific, and that they are regulated in a stage-specific manner during spermatogenesis. We generated antibodies against the proteins encoded by seven of the genes to facilitate their characterization in male germ cells. Immunoblotting and immunofluorescence analyses revealed that one of these proteins was expressed only in testicular germ cells, three were expressed in both testicular germ cells and testicular sperm, and the remaining three were expressed in sperm of the testicular stages and in mature sperm from the epididymis. Further analysis of the latter three proteins showed that they were all associated with cytoskeletal structures in the sperm flagellum. Among them, MORN5, which is predicted to contain three MORN motifs, is conserved between mouse and human sperm. In conclusion, we herein identify 13 authentic genes with male germ cell-specific expression, and provide comprehensive information about these genes and their encoded products. Our finding will facilitate future investigations into the functional roles of these novel genes in spermatogenesis and sperm functions.http://europepmc.org/articles/PMC5526581?pdf=render |
| spellingShingle | Jun Tae Kwon Sera Ham Suyeon Jeon Youil Kim Seungmin Oh Chunghee Cho Expression of uncharacterized male germ cell-specific genes and discovery of novel sperm-tail proteins in mice. PLoS ONE |
| title | Expression of uncharacterized male germ cell-specific genes and discovery of novel sperm-tail proteins in mice. |
| title_full | Expression of uncharacterized male germ cell-specific genes and discovery of novel sperm-tail proteins in mice. |
| title_fullStr | Expression of uncharacterized male germ cell-specific genes and discovery of novel sperm-tail proteins in mice. |
| title_full_unstemmed | Expression of uncharacterized male germ cell-specific genes and discovery of novel sperm-tail proteins in mice. |
| title_short | Expression of uncharacterized male germ cell-specific genes and discovery of novel sperm-tail proteins in mice. |
| title_sort | expression of uncharacterized male germ cell specific genes and discovery of novel sperm tail proteins in mice |
| url | http://europepmc.org/articles/PMC5526581?pdf=render |
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