Molecular Genomic Study of Inhibin Molecule Production through Granulosa Cell Gene Expression in Inhibin-Deficient Mice
Inhibin is a molecule that belongs to peptide hormones and is excreted through pituitary gonadotropins stimulation action on the granulosa cells of the ovaries. However, the differential regulation of inhibin and follicle-stimulating hormone (FSH) on granulosa cell tumor growth in mice inhibin-defic...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/17/5595 |
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| author | Hira Sajjad Talpur Zia ur Rehman Mostafa Gouda Aixing Liang Iqra Bano Mir Sajjad Hussain FarmanUllah FarmanUllah Liguo Yang |
| author_facet | Hira Sajjad Talpur Zia ur Rehman Mostafa Gouda Aixing Liang Iqra Bano Mir Sajjad Hussain FarmanUllah FarmanUllah Liguo Yang |
| author_sort | Hira Sajjad Talpur |
| collection | DOAJ |
| description | Inhibin is a molecule that belongs to peptide hormones and is excreted through pituitary gonadotropins stimulation action on the granulosa cells of the ovaries. However, the differential regulation of inhibin and follicle-stimulating hormone (FSH) on granulosa cell tumor growth in mice inhibin-deficient females is not yet well understood. The objective of this study was to evaluate the role of inhibin and FSH on the granulosa cells of ovarian follicles at the premature antral stage. This study stimulated immature wild-type (WT) and Inhibin-α knockout (Inha−/−) female mice with human chorionic gonadotropin (hCG) and examined hCG-induced gene expression changes in granulosa cells. Also, screening of differentially expressed genes (DEGs) was performed in the two groups under study. In addition, related modules to external traits and key gene drivers were determined through Weighted Gene Co-Expression Network Analysis (WGCNA) algorithm. The results identified a number of 1074 and 931 DEGs and 343 overlapping DEGs (ODEGs) were shared in the two groups. Some 341 ODEGs had high relevance and consistent expression direction, with a significant correlation coefficient (r<sup>2</sup> = 0.9145). Additionally, the gene co-expression network of selected 153 genes showed 122 nodes enriched to 21 GO biological processes (BP) and reproduction and 3 genes related to genomic pathways. By using principal component analysis (PCA), the 14 genes in the regulatory network were fixed and the cumulative proportion of fitted top three principal components was 94.64%. In conclusion, this study revealed the novelty of using ODEGs for investigating the inhibin and FSH hormone pathways that might open the way toward gene therapy for granulosa cell tumors. Also, these genes could be used as biomarkers for tracking the changes in inhibin and FSH hormone from the changes in the nutrition pattern. |
| first_indexed | 2024-03-10T01:29:32Z |
| format | Article |
| id | doaj.art-46557ec0b9cb44fcb0535b3f11e90603 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T01:29:32Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-46557ec0b9cb44fcb0535b3f11e906032023-11-23T13:44:49ZengMDPI AGMolecules1420-30492022-08-012717559510.3390/molecules27175595Molecular Genomic Study of Inhibin Molecule Production through Granulosa Cell Gene Expression in Inhibin-Deficient MiceHira Sajjad Talpur0Zia ur Rehman1Mostafa Gouda2Aixing Liang3Iqra Bano4Mir Sajjad Hussain5FarmanUllah FarmanUllah6Liguo Yang7National Center for International Research on Animal Genetics, Breeding and Reproduction (NCIRAGBR), Huazhong Agricultural University, Ministry of Science and Technology of the People’s Republic of China, Wuhan 430070, ChinaNational Center for International Research on Animal Genetics, Breeding and Reproduction (NCIRAGBR), Huazhong Agricultural University, Ministry of Science and Technology of the People’s Republic of China, Wuhan 430070, ChinaCollege of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, ChinaNational Center for International Research on Animal Genetics, Breeding and Reproduction (NCIRAGBR), Huazhong Agricultural University, Ministry of Science and Technology of the People’s Republic of China, Wuhan 430070, ChinaDepartment of Animal Breeding and Genetics, Sindh Agriculture University, Tandojam 70060, Sindh, PakistanDepartment of Animal Breeding and Genetics, Sindh Agriculture University, Tandojam 70060, Sindh, PakistanNational Center for International Research on Animal Genetics, Breeding and Reproduction (NCIRAGBR), Huazhong Agricultural University, Ministry of Science and Technology of the People’s Republic of China, Wuhan 430070, ChinaNational Center for International Research on Animal Genetics, Breeding and Reproduction (NCIRAGBR), Huazhong Agricultural University, Ministry of Science and Technology of the People’s Republic of China, Wuhan 430070, ChinaInhibin is a molecule that belongs to peptide hormones and is excreted through pituitary gonadotropins stimulation action on the granulosa cells of the ovaries. However, the differential regulation of inhibin and follicle-stimulating hormone (FSH) on granulosa cell tumor growth in mice inhibin-deficient females is not yet well understood. The objective of this study was to evaluate the role of inhibin and FSH on the granulosa cells of ovarian follicles at the premature antral stage. This study stimulated immature wild-type (WT) and Inhibin-α knockout (Inha−/−) female mice with human chorionic gonadotropin (hCG) and examined hCG-induced gene expression changes in granulosa cells. Also, screening of differentially expressed genes (DEGs) was performed in the two groups under study. In addition, related modules to external traits and key gene drivers were determined through Weighted Gene Co-Expression Network Analysis (WGCNA) algorithm. The results identified a number of 1074 and 931 DEGs and 343 overlapping DEGs (ODEGs) were shared in the two groups. Some 341 ODEGs had high relevance and consistent expression direction, with a significant correlation coefficient (r<sup>2</sup> = 0.9145). Additionally, the gene co-expression network of selected 153 genes showed 122 nodes enriched to 21 GO biological processes (BP) and reproduction and 3 genes related to genomic pathways. By using principal component analysis (PCA), the 14 genes in the regulatory network were fixed and the cumulative proportion of fitted top three principal components was 94.64%. In conclusion, this study revealed the novelty of using ODEGs for investigating the inhibin and FSH hormone pathways that might open the way toward gene therapy for granulosa cell tumors. Also, these genes could be used as biomarkers for tracking the changes in inhibin and FSH hormone from the changes in the nutrition pattern.https://www.mdpi.com/1420-3049/27/17/5595FSHDEGsgranulosa cellshCGinhibinInha |
| spellingShingle | Hira Sajjad Talpur Zia ur Rehman Mostafa Gouda Aixing Liang Iqra Bano Mir Sajjad Hussain FarmanUllah FarmanUllah Liguo Yang Molecular Genomic Study of Inhibin Molecule Production through Granulosa Cell Gene Expression in Inhibin-Deficient Mice Molecules FSH DEGs granulosa cells hCG inhibin Inha |
| title | Molecular Genomic Study of Inhibin Molecule Production through Granulosa Cell Gene Expression in Inhibin-Deficient Mice |
| title_full | Molecular Genomic Study of Inhibin Molecule Production through Granulosa Cell Gene Expression in Inhibin-Deficient Mice |
| title_fullStr | Molecular Genomic Study of Inhibin Molecule Production through Granulosa Cell Gene Expression in Inhibin-Deficient Mice |
| title_full_unstemmed | Molecular Genomic Study of Inhibin Molecule Production through Granulosa Cell Gene Expression in Inhibin-Deficient Mice |
| title_short | Molecular Genomic Study of Inhibin Molecule Production through Granulosa Cell Gene Expression in Inhibin-Deficient Mice |
| title_sort | molecular genomic study of inhibin molecule production through granulosa cell gene expression in inhibin deficient mice |
| topic | FSH DEGs granulosa cells hCG inhibin Inha |
| url | https://www.mdpi.com/1420-3049/27/17/5595 |
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