Identification of amylase activity from vannamei shrimps’ (Litopenaeus vannamei) digestive tract using size exclusion chromatography method
Vannamei shrimps (Litopenaeus vannamei), also known as white leg shrimps are widely cultivated and consumed by many people. However, most consumers have removed the shrimp’s head which integrates with its digestive tract. The digestive tract of white leg shrimp contains digestive enzymes, including...
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| Format: | Article |
| Language: | English |
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Universitas Syiah Kuala, Faculty of Mathematics and Natural Science
2021-10-01
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| Series: | Jurnal Natural |
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| Online Access: | http://jurnal.unsyiah.ac.id/natural/article/view/18874 |
| _version_ | 1797229912769167360 |
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| author | HARIYANTI HARIYANTI HANIFAH RAHMI |
| author_facet | HARIYANTI HARIYANTI HANIFAH RAHMI |
| author_sort | HARIYANTI HARIYANTI |
| collection | DOAJ |
| description | Vannamei shrimps (Litopenaeus vannamei), also known as white leg shrimps are widely cultivated and consumed by many people. However, most consumers have removed the shrimp’s head which integrates with its digestive tract. The digestive tract of white leg shrimp contains digestive enzymes, including amylase. This study aimed to determine the protein content and amylase activity from Vannamei shrimps’ digestive tract using the size exclusion chromatography method. The protein isolation of size exclusion chromatography was prepared in three steps, namely; centrifugation, precipitation, and dialysis. The protein from the dialysis step was purified by using gel filtration chromatography. Each obtained fraction was determined the protein content and amylase activity by using spectrophotometer UV-Vis method. The results showed that the fraction of 108th had the highest protein content and amylase activity with a value of 0.85 mg/ml and 25.66U/ml respectively. @font-face {font-family:"Cambria Math"; panose-1:2 4 5 3 5 4 6 3 2 4; mso-font-charset:0; mso-generic-font-family:roman; mso-font-pitch:variable; mso-font-signature:-536869121 1107305727 33554432 0 415 0;}p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-unhide:no; mso-style-qformat:yes; mso-style-parent:""; margin:0cm; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman",serif; mso-fareast-font-family:"Times New Roman"; mso-ansi-language:EN-US;}.MsoChpDefault {mso-style-type:export-only; mso-default-props:yes; font-size:10.0pt; mso-ansi-font-size:10.0pt; mso-bidi-font-size:10.0pt; mso-ansi-language:IN; mso-fareast-language:IN;}div.WordSection1 {page:WordSection1;} |
| first_indexed | 2024-04-24T15:20:08Z |
| format | Article |
| id | doaj.art-465aa35384c8477fb61874dba2003c56 |
| institution | Directory Open Access Journal |
| issn | 1411-8513 2541-4062 |
| language | English |
| last_indexed | 2024-04-24T15:20:08Z |
| publishDate | 2021-10-01 |
| publisher | Universitas Syiah Kuala, Faculty of Mathematics and Natural Science |
| record_format | Article |
| series | Jurnal Natural |
| spelling | doaj.art-465aa35384c8477fb61874dba2003c562024-04-02T08:19:40ZengUniversitas Syiah Kuala, Faculty of Mathematics and Natural ScienceJurnal Natural1411-85132541-40622021-10-0121312312710.24815/jn.v21i3.1887413400Identification of amylase activity from vannamei shrimps’ (Litopenaeus vannamei) digestive tract using size exclusion chromatography methodHARIYANTI HARIYANTI0HANIFAH RAHMI1Universitas Muhammadiyah Prof. DR. HAMKAUniversitas Muhammadiyah Prof. DR. HAMKAVannamei shrimps (Litopenaeus vannamei), also known as white leg shrimps are widely cultivated and consumed by many people. However, most consumers have removed the shrimp’s head which integrates with its digestive tract. The digestive tract of white leg shrimp contains digestive enzymes, including amylase. This study aimed to determine the protein content and amylase activity from Vannamei shrimps’ digestive tract using the size exclusion chromatography method. The protein isolation of size exclusion chromatography was prepared in three steps, namely; centrifugation, precipitation, and dialysis. The protein from the dialysis step was purified by using gel filtration chromatography. Each obtained fraction was determined the protein content and amylase activity by using spectrophotometer UV-Vis method. The results showed that the fraction of 108th had the highest protein content and amylase activity with a value of 0.85 mg/ml and 25.66U/ml respectively. @font-face {font-family:"Cambria Math"; panose-1:2 4 5 3 5 4 6 3 2 4; mso-font-charset:0; mso-generic-font-family:roman; mso-font-pitch:variable; mso-font-signature:-536869121 1107305727 33554432 0 415 0;}p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-unhide:no; mso-style-qformat:yes; mso-style-parent:""; margin:0cm; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman",serif; mso-fareast-font-family:"Times New Roman"; mso-ansi-language:EN-US;}.MsoChpDefault {mso-style-type:export-only; mso-default-props:yes; font-size:10.0pt; mso-ansi-font-size:10.0pt; mso-bidi-font-size:10.0pt; mso-ansi-language:IN; mso-fareast-language:IN;}div.WordSection1 {page:WordSection1;}http://jurnal.unsyiah.ac.id/natural/article/view/18874ammonium sulphate, amylase, size exclusion chromatography, vannamei shrimp |
| spellingShingle | HARIYANTI HARIYANTI HANIFAH RAHMI Identification of amylase activity from vannamei shrimps’ (Litopenaeus vannamei) digestive tract using size exclusion chromatography method Jurnal Natural ammonium sulphate, amylase, size exclusion chromatography, vannamei shrimp |
| title | Identification of amylase activity from vannamei shrimps’ (Litopenaeus vannamei) digestive tract using size exclusion chromatography method |
| title_full | Identification of amylase activity from vannamei shrimps’ (Litopenaeus vannamei) digestive tract using size exclusion chromatography method |
| title_fullStr | Identification of amylase activity from vannamei shrimps’ (Litopenaeus vannamei) digestive tract using size exclusion chromatography method |
| title_full_unstemmed | Identification of amylase activity from vannamei shrimps’ (Litopenaeus vannamei) digestive tract using size exclusion chromatography method |
| title_short | Identification of amylase activity from vannamei shrimps’ (Litopenaeus vannamei) digestive tract using size exclusion chromatography method |
| title_sort | identification of amylase activity from vannamei shrimps litopenaeus vannamei digestive tract using size exclusion chromatography method |
| topic | ammonium sulphate, amylase, size exclusion chromatography, vannamei shrimp |
| url | http://jurnal.unsyiah.ac.id/natural/article/view/18874 |
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