Expression Profiling of Lipolytic and Long-Chain Fatty Acid Biosynthesis Genes in Catfish Larvae Fed With Graded Lipid Levels
This study was designed to evaluate the effect of dietary lipid levels on the mRNA transcripts of lipid metabolic regulatory genes. Nine combinations of experimental diets containing graded lipid (80, 100, and 120 g/kg) and protein (450, 500, and 550 g/kg) levels were fed to the Clarias magur (India...
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Frontiers Media S.A.
2020-09-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmars.2020.561402/full |
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| author | Ishfaq Nazir Mir P. P. Srivastava Irfan Ahmad Bhat Jaffer Yousuf Dar Deepak Agarwal |
| author_facet | Ishfaq Nazir Mir P. P. Srivastava Irfan Ahmad Bhat Jaffer Yousuf Dar Deepak Agarwal |
| author_sort | Ishfaq Nazir Mir |
| collection | DOAJ |
| description | This study was designed to evaluate the effect of dietary lipid levels on the mRNA transcripts of lipid metabolic regulatory genes. Nine combinations of experimental diets containing graded lipid (80, 100, and 120 g/kg) and protein (450, 500, and 550 g/kg) levels were fed to the Clarias magur (Indian walking catfish) larvae from 14 to 35 dph (day post hatching). All the lipolytic genes, such as pancreatic triacylglycerol lipase (PL), lipoprotein lipase (LPL), and bile salt-activated lipase (BAL), and genes for long-chain polyunsaturated fatty acid (LC-PUFA) biosynthetic enzymes like fatty acyl desaturase-2 (FADS2), fatty acyl desaturase-5 (FADS5), and elongase (ELOV) were expressed in a wide range of tissues. A high abundance of mRNA transcript levels of lipolytic genes was detected in the intestine and liver, and similarly, desaturases and elongase were predominantly found to be expressed in the liver, brain, and intestine. Among the diets, a significantly high expression of both lipolytic and LC-PUFA biosynthetic genes were observed at 8% dietary lipid level. The mRNA expression of all the studied genes was down-regulated at 12% dietary lipid contents. Hence, the present study concludes that the efficient nutrient utilization and the lipid metabolic pathway occur at the optimum dietary lipid level of 8% in C. magur larvae. |
| first_indexed | 2024-12-13T01:22:12Z |
| format | Article |
| id | doaj.art-465b27b392004d0fab248e92b9367ab2 |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-12-13T01:22:12Z |
| publishDate | 2020-09-01 |
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| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj.art-465b27b392004d0fab248e92b9367ab22022-12-22T00:04:13ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452020-09-01710.3389/fmars.2020.561402561402Expression Profiling of Lipolytic and Long-Chain Fatty Acid Biosynthesis Genes in Catfish Larvae Fed With Graded Lipid LevelsIshfaq Nazir Mir0P. P. Srivastava1Irfan Ahmad Bhat2Jaffer Yousuf Dar3Deepak Agarwal4DIVA, Tamil Nadu Dr. J. Jayalalithaa Fisheries University, Chennai, IndiaDivision of Fish Nutrition, Biochemistry and Physiology, ICAR-Central Institute of Fisheries Education, Mumbai, IndiaCollege of Fisheries Science, Gumla, Birsa Agricultural University, Ranchi, IndiaICAR-Central Soil Salinity Research Institute, Karnal, IndiaIFPGS, Tamil Nadu Dr. J. Jayalalithaa Fisheries University, Chennai, IndiaThis study was designed to evaluate the effect of dietary lipid levels on the mRNA transcripts of lipid metabolic regulatory genes. Nine combinations of experimental diets containing graded lipid (80, 100, and 120 g/kg) and protein (450, 500, and 550 g/kg) levels were fed to the Clarias magur (Indian walking catfish) larvae from 14 to 35 dph (day post hatching). All the lipolytic genes, such as pancreatic triacylglycerol lipase (PL), lipoprotein lipase (LPL), and bile salt-activated lipase (BAL), and genes for long-chain polyunsaturated fatty acid (LC-PUFA) biosynthetic enzymes like fatty acyl desaturase-2 (FADS2), fatty acyl desaturase-5 (FADS5), and elongase (ELOV) were expressed in a wide range of tissues. A high abundance of mRNA transcript levels of lipolytic genes was detected in the intestine and liver, and similarly, desaturases and elongase were predominantly found to be expressed in the liver, brain, and intestine. Among the diets, a significantly high expression of both lipolytic and LC-PUFA biosynthetic genes were observed at 8% dietary lipid level. The mRNA expression of all the studied genes was down-regulated at 12% dietary lipid contents. Hence, the present study concludes that the efficient nutrient utilization and the lipid metabolic pathway occur at the optimum dietary lipid level of 8% in C. magur larvae.https://www.frontiersin.org/article/10.3389/fmars.2020.561402/fulldietary lipidlipolyticdesaturaseelongasetranscriptClarias magur |
| spellingShingle | Ishfaq Nazir Mir P. P. Srivastava Irfan Ahmad Bhat Jaffer Yousuf Dar Deepak Agarwal Expression Profiling of Lipolytic and Long-Chain Fatty Acid Biosynthesis Genes in Catfish Larvae Fed With Graded Lipid Levels Frontiers in Marine Science dietary lipid lipolytic desaturase elongase transcript Clarias magur |
| title | Expression Profiling of Lipolytic and Long-Chain Fatty Acid Biosynthesis Genes in Catfish Larvae Fed With Graded Lipid Levels |
| title_full | Expression Profiling of Lipolytic and Long-Chain Fatty Acid Biosynthesis Genes in Catfish Larvae Fed With Graded Lipid Levels |
| title_fullStr | Expression Profiling of Lipolytic and Long-Chain Fatty Acid Biosynthesis Genes in Catfish Larvae Fed With Graded Lipid Levels |
| title_full_unstemmed | Expression Profiling of Lipolytic and Long-Chain Fatty Acid Biosynthesis Genes in Catfish Larvae Fed With Graded Lipid Levels |
| title_short | Expression Profiling of Lipolytic and Long-Chain Fatty Acid Biosynthesis Genes in Catfish Larvae Fed With Graded Lipid Levels |
| title_sort | expression profiling of lipolytic and long chain fatty acid biosynthesis genes in catfish larvae fed with graded lipid levels |
| topic | dietary lipid lipolytic desaturase elongase transcript Clarias magur |
| url | https://www.frontiersin.org/article/10.3389/fmars.2020.561402/full |
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