Interleukin Gene Variability and Periodontal Bacteria in Patients with Generalized Aggressive Form of Periodontitis
Host genetic predispositions to dysregulated immune response can influence the development of the aggressive form of periodontitis (AgP) through susceptibility to oral dysbiosis and subsequent host-microbe interaction. This case-control study aimed to perform a multilocus analysis of functional vari...
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MDPI AG
2020-07-01
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| Series: | International Journal of Molecular Sciences |
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| author | Petra Borilova Linhartova Zdenek Danek Tereza Deissova Filip Hromcik Bretislav Lipovy David Szaraz Julius Janos Antonin Fassmann Jirina Bartova Ivo Drizhal Lydie Izakovicova Holla |
| author_facet | Petra Borilova Linhartova Zdenek Danek Tereza Deissova Filip Hromcik Bretislav Lipovy David Szaraz Julius Janos Antonin Fassmann Jirina Bartova Ivo Drizhal Lydie Izakovicova Holla |
| author_sort | Petra Borilova Linhartova |
| collection | DOAJ |
| description | Host genetic predispositions to dysregulated immune response can influence the development of the aggressive form of periodontitis (AgP) through susceptibility to oral dysbiosis and subsequent host-microbe interaction. This case-control study aimed to perform a multilocus analysis of functional variants in selected interleukin (<i>IL</i>) genes in patients with the generalized form of AgP in a homogenous population. Twelve polymorphisms in <i>IL-1</i> gene cluster, <i>IL-6</i> and its receptor, <i>IL-10</i>, <i>IL-17A</i>, and <i>IL-18</i> were determined in 91 AgP patients and 210 controls. Analysis of seven selected periodontal bacteria in subgingival sulci/pockets was performed with a commercial DNA-microarray kit in a subgroup of 76 individuals. The pilot in vitro study included stimulation of peripheral blood monocytes (PBMC) from 20 individuals with periodontal bacteria and measurement of IL-10 levels using the Luminex method. Only the unctional polymorphism <i>IL-10</i> −1087 A/G (rs1800896) and specific <i>IL-10</i> haplotypes were associated with the development of the disease (<i>p</i> < 0.05, <i>P</i><sub>corr</sub> > 0.05). Four bacterial species occurred more frequently in AgP than in controls (<i>p</i> < 0.01, <i>P</i><sub>corr</sub> < 0.05). Elevated IL-10 levels were found in AgP patients, carriers of <i>IL-10</i> −1087GG genotype, and PBMCs stimulated by periodontal bacteria (<i>p</i> < 0.05, <i>P</i><sub>corr</sub> > 0.05). We therefore conclude that a combination of genetic predisposition to the altered expression of <i>IL-10</i> and the presence of specific periodontal bacteria may contribute to Th1/Th2 balance disruption and AgP development. |
| first_indexed | 2024-03-10T18:43:25Z |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
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| spelling | doaj.art-465be9ad8984477f9dc826efcf86c6f42023-11-20T05:41:53ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-07-012113472810.3390/ijms21134728Interleukin Gene Variability and Periodontal Bacteria in Patients with Generalized Aggressive Form of PeriodontitisPetra Borilova Linhartova0Zdenek Danek1Tereza Deissova2Filip Hromcik3Bretislav Lipovy4David Szaraz5Julius Janos6Antonin Fassmann7Jirina Bartova8Ivo Drizhal9Lydie Izakovicova Holla10Clinic of Stomatology, Faculty of Medicine, Masaryk University, Pekarska 664/53, 60200 Brno, Czech RepublicDepartment of Pathophysiology, Faculty of Medicine, Masaryk University, Kamenice 5, 62500 Brno, Czech RepublicDepartment of Pathophysiology, Faculty of Medicine, Masaryk University, Kamenice 5, 62500 Brno, Czech RepublicClinic of Stomatology, Faculty of Medicine, Masaryk University, Pekarska 664/53, 60200 Brno, Czech RepublicDepartment of Pathophysiology, Faculty of Medicine, Masaryk University, Kamenice 5, 62500 Brno, Czech RepublicClinic of Maxillofacial Surgery, University Hospital Brno, Jihlavska 20, 62500 Brno, Czech RepublicClinic of Stomatology, Faculty of Medicine, Masaryk University, Pekarska 664/53, 60200 Brno, Czech RepublicClinic of Stomatology, St. Anne’s University Hospital, Pekarska 664/53, 60200 Brno, Czech RepublicInstitute of Clinical and Experimental Dental Medicine, First Faculty of Medicine, Charles University, Karlovo nam. 554/32, 12808 Prague, Czech RepublicDepartment of Dentistry, Faculty of Medicine in Hradec Kralove, Charles University, Simkova 870, 50003 Hradec Kralove, Czech RepublicClinic of Stomatology, Faculty of Medicine, Masaryk University, Pekarska 664/53, 60200 Brno, Czech RepublicHost genetic predispositions to dysregulated immune response can influence the development of the aggressive form of periodontitis (AgP) through susceptibility to oral dysbiosis and subsequent host-microbe interaction. This case-control study aimed to perform a multilocus analysis of functional variants in selected interleukin (<i>IL</i>) genes in patients with the generalized form of AgP in a homogenous population. Twelve polymorphisms in <i>IL-1</i> gene cluster, <i>IL-6</i> and its receptor, <i>IL-10</i>, <i>IL-17A</i>, and <i>IL-18</i> were determined in 91 AgP patients and 210 controls. Analysis of seven selected periodontal bacteria in subgingival sulci/pockets was performed with a commercial DNA-microarray kit in a subgroup of 76 individuals. The pilot in vitro study included stimulation of peripheral blood monocytes (PBMC) from 20 individuals with periodontal bacteria and measurement of IL-10 levels using the Luminex method. Only the unctional polymorphism <i>IL-10</i> −1087 A/G (rs1800896) and specific <i>IL-10</i> haplotypes were associated with the development of the disease (<i>p</i> < 0.05, <i>P</i><sub>corr</sub> > 0.05). Four bacterial species occurred more frequently in AgP than in controls (<i>p</i> < 0.01, <i>P</i><sub>corr</sub> < 0.05). Elevated IL-10 levels were found in AgP patients, carriers of <i>IL-10</i> −1087GG genotype, and PBMCs stimulated by periodontal bacteria (<i>p</i> < 0.05, <i>P</i><sub>corr</sub> > 0.05). We therefore conclude that a combination of genetic predisposition to the altered expression of <i>IL-10</i> and the presence of specific periodontal bacteria may contribute to Th1/Th2 balance disruption and AgP development.https://www.mdpi.com/1422-0067/21/13/4728aggressive periodontitisoral bacteriainflammationinterleukinpolymorphismgenetic predisposition |
| spellingShingle | Petra Borilova Linhartova Zdenek Danek Tereza Deissova Filip Hromcik Bretislav Lipovy David Szaraz Julius Janos Antonin Fassmann Jirina Bartova Ivo Drizhal Lydie Izakovicova Holla Interleukin Gene Variability and Periodontal Bacteria in Patients with Generalized Aggressive Form of Periodontitis International Journal of Molecular Sciences aggressive periodontitis oral bacteria inflammation interleukin polymorphism genetic predisposition |
| title | Interleukin Gene Variability and Periodontal Bacteria in Patients with Generalized Aggressive Form of Periodontitis |
| title_full | Interleukin Gene Variability and Periodontal Bacteria in Patients with Generalized Aggressive Form of Periodontitis |
| title_fullStr | Interleukin Gene Variability and Periodontal Bacteria in Patients with Generalized Aggressive Form of Periodontitis |
| title_full_unstemmed | Interleukin Gene Variability and Periodontal Bacteria in Patients with Generalized Aggressive Form of Periodontitis |
| title_short | Interleukin Gene Variability and Periodontal Bacteria in Patients with Generalized Aggressive Form of Periodontitis |
| title_sort | interleukin gene variability and periodontal bacteria in patients with generalized aggressive form of periodontitis |
| topic | aggressive periodontitis oral bacteria inflammation interleukin polymorphism genetic predisposition |
| url | https://www.mdpi.com/1422-0067/21/13/4728 |
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