Otimização e validação intralaboratorial de método para análise de resíduos de cloranfenicol em leite caprino por cromatografia gasosa com detecção por captura de elétrons (CG/DCE) Optimization and intralaboratorial validation of method for analysis of chloramphenicol residues in goat milk by GC/ECD

Otimização e validação intralaboratorial de método para análise de resíduos de cloranfenicol em leite caprino por cromatografia gasosa com detecção por captura de elétrons (CG/DCE) Optimization and intralaboratorial validation of method for analysis of chloramphenicol residues in goat milk by GC/ECD

<abstract language="eng">The presence of chloramphenicol residues in goat milk can cause toxic effects in the population. The present work consists of the optimization and validation of analytical methodology for determination of chloramphenicol residues in goat milk by GC/ECD. The e...

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Bibliographic Details
Main Authors: Jaff R. da Silva, Luciana T. Silva, Janice I. Druzian
Format: Article
Language:English
Published: Sociedade Brasileira de Química 2010-01-01
Series:Química Nova
Subjects:
chloramphenicol residues
goat milk
GC
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422010000100017
Description
Summary:<abstract language="eng">The presence of chloramphenicol residues in goat milk can cause toxic effects in the population. The present work consists of the optimization and validation of analytical methodology for determination of chloramphenicol residues in goat milk by GC/ECD. The extraction was made with ethyl acetate and the clean-up with SPE-C18. The identification was made by comparison of retention time and GC/MS, and the quantification by external standard. The method was selective, linearity (0.998), precise (5.8-13.4%), exact (69.87-73.71%) and robust. The LOD and LOQ of method were 0.030 and 0.10 &#956;g/kg, respectively. The method was efficiently for analysis of chloramphenicol in goat milk.
ISSN:0100-4042
1678-7064

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