Otimização e validação intralaboratorial de método para análise de resíduos de cloranfenicol em leite caprino por cromatografia gasosa com detecção por captura de elétrons (CG/DCE) Optimization and intralaboratorial validation of method for analysis of chloramphenicol residues in goat milk by GC/ECD
<abstract language="eng">The presence of chloramphenicol residues in goat milk can cause toxic effects in the population. The present work consists of the optimization and validation of analytical methodology for determination of chloramphenicol residues in goat milk by GC/ECD. The e...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sociedade Brasileira de Química
2010-01-01
|
| Series: | Química Nova |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422010000100017 |
| _version_ | 1818112917860515840 |
|---|---|
| author | Jaff R. da Silva Luciana T. Silva Janice I. Druzian |
| author_facet | Jaff R. da Silva Luciana T. Silva Janice I. Druzian |
| author_sort | Jaff R. da Silva |
| collection | DOAJ |
| description | <abstract language="eng">The presence of chloramphenicol residues in goat milk can cause toxic effects in the population. The present work consists of the optimization and validation of analytical methodology for determination of chloramphenicol residues in goat milk by GC/ECD. The extraction was made with ethyl acetate and the clean-up with SPE-C18. The identification was made by comparison of retention time and GC/MS, and the quantification by external standard. The method was selective, linearity (0.998), precise (5.8-13.4%), exact (69.87-73.71%) and robust. The LOD and LOQ of method were 0.030 and 0.10 μg/kg, respectively. The method was efficiently for analysis of chloramphenicol in goat milk. |
| first_indexed | 2024-12-11T03:26:34Z |
| format | Article |
| id | doaj.art-465d35ee90544e8592614bb47d7456f8 |
| institution | Directory Open Access Journal |
| issn | 0100-4042 1678-7064 |
| language | English |
| last_indexed | 2024-12-11T03:26:34Z |
| publishDate | 2010-01-01 |
| publisher | Sociedade Brasileira de Química |
| record_format | Article |
| series | Química Nova |
| spelling | doaj.art-465d35ee90544e8592614bb47d7456f82022-12-22T01:22:29ZengSociedade Brasileira de QuímicaQuímica Nova0100-40421678-70642010-01-01331909610.1590/S0100-40422010000100017Otimização e validação intralaboratorial de método para análise de resíduos de cloranfenicol em leite caprino por cromatografia gasosa com detecção por captura de elétrons (CG/DCE) Optimization and intralaboratorial validation of method for analysis of chloramphenicol residues in goat milk by GC/ECDJaff R. da SilvaLuciana T. SilvaJanice I. Druzian<abstract language="eng">The presence of chloramphenicol residues in goat milk can cause toxic effects in the population. The present work consists of the optimization and validation of analytical methodology for determination of chloramphenicol residues in goat milk by GC/ECD. The extraction was made with ethyl acetate and the clean-up with SPE-C18. The identification was made by comparison of retention time and GC/MS, and the quantification by external standard. The method was selective, linearity (0.998), precise (5.8-13.4%), exact (69.87-73.71%) and robust. The LOD and LOQ of method were 0.030 and 0.10 μg/kg, respectively. The method was efficiently for analysis of chloramphenicol in goat milk.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422010000100017chloramphenicol residuesgoat milkGC |
| spellingShingle | Jaff R. da Silva Luciana T. Silva Janice I. Druzian Otimização e validação intralaboratorial de método para análise de resíduos de cloranfenicol em leite caprino por cromatografia gasosa com detecção por captura de elétrons (CG/DCE) Optimization and intralaboratorial validation of method for analysis of chloramphenicol residues in goat milk by GC/ECD Química Nova chloramphenicol residues goat milk GC |
| title | Otimização e validação intralaboratorial de método para análise de resíduos de cloranfenicol em leite caprino por cromatografia gasosa com detecção por captura de elétrons (CG/DCE) Optimization and intralaboratorial validation of method for analysis of chloramphenicol residues in goat milk by GC/ECD |
| title_full | Otimização e validação intralaboratorial de método para análise de resíduos de cloranfenicol em leite caprino por cromatografia gasosa com detecção por captura de elétrons (CG/DCE) Optimization and intralaboratorial validation of method for analysis of chloramphenicol residues in goat milk by GC/ECD |
| title_fullStr | Otimização e validação intralaboratorial de método para análise de resíduos de cloranfenicol em leite caprino por cromatografia gasosa com detecção por captura de elétrons (CG/DCE) Optimization and intralaboratorial validation of method for analysis of chloramphenicol residues in goat milk by GC/ECD |
| title_full_unstemmed | Otimização e validação intralaboratorial de método para análise de resíduos de cloranfenicol em leite caprino por cromatografia gasosa com detecção por captura de elétrons (CG/DCE) Optimization and intralaboratorial validation of method for analysis of chloramphenicol residues in goat milk by GC/ECD |
| title_short | Otimização e validação intralaboratorial de método para análise de resíduos de cloranfenicol em leite caprino por cromatografia gasosa com detecção por captura de elétrons (CG/DCE) Optimization and intralaboratorial validation of method for analysis of chloramphenicol residues in goat milk by GC/ECD |
| title_sort | otimizacao e validacao intralaboratorial de metodo para analise de residuos de cloranfenicol em leite caprino por cromatografia gasosa com deteccao por captura de eletrons cg dce optimization and intralaboratorial validation of method for analysis of chloramphenicol residues in goat milk by gc ecd |
| topic | chloramphenicol residues goat milk GC |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422010000100017 |
| work_keys_str_mv | AT jaffrdasilva otimizacaoevalidacaointralaboratorialdemetodoparaanalisederesiduosdecloranfenicolemleitecaprinoporcromatografiagasosacomdeteccaoporcapturadeeletronscgdceoptimizationandintralaboratorialvalidationofmethodforanalysisofchloramphenicolresiduesingoatmilkbygcec AT lucianatsilva otimizacaoevalidacaointralaboratorialdemetodoparaanalisederesiduosdecloranfenicolemleitecaprinoporcromatografiagasosacomdeteccaoporcapturadeeletronscgdceoptimizationandintralaboratorialvalidationofmethodforanalysisofchloramphenicolresiduesingoatmilkbygcec AT janiceidruzian otimizacaoevalidacaointralaboratorialdemetodoparaanalisederesiduosdecloranfenicolemleitecaprinoporcromatografiagasosacomdeteccaoporcapturadeeletronscgdceoptimizationandintralaboratorialvalidationofmethodforanalysisofchloramphenicolresiduesingoatmilkbygcec |