Effects of Salt Stress on the Antioxidant Activity and Malondialdehyde, Solution Protein, Proline, and Chlorophyll Contents of Three <i>Malus</i> Species
Understanding the different physiological responses of <i>Malus</i> species under salt stress in the seedling stages will be useful in breeding salt-tolerant dwarfing apple rootstocks. Seedlings of <i>Malus Zumi</i> (Mats.) Rehd. (<i>M. zumi</i>), <i>Malus s...
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MDPI AG
2022-11-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/12/11/1929 |
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| author | Dajiang Wang Yuan Gao Simiao Sun Xiang Lu Qingshan Li Lianwen Li Kun Wang Jihong Liu |
| author_facet | Dajiang Wang Yuan Gao Simiao Sun Xiang Lu Qingshan Li Lianwen Li Kun Wang Jihong Liu |
| author_sort | Dajiang Wang |
| collection | DOAJ |
| description | Understanding the different physiological responses of <i>Malus</i> species under salt stress in the seedling stages will be useful in breeding salt-tolerant dwarfing apple rootstocks. Seedlings of <i>Malus Zumi</i> (Mats.) Rehd. (<i>M. zumi</i>), <i>Malus sieversii</i> (Led.) Roem. (<i>M. sieversii</i>), and <i>Malus baccata</i> (L.) Borkh. (<i>M. baccata</i>) were treated with solution of 0, 0.20%, 0.40%, and 0.60% salinity. Physiological parameters of their leaves and roots were measured at 0 d, 4 d, 8 d and 12 d after salinity treatments. Superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), malondialdehyde (MDA), solution protein (SP), and proline (PRO) initially increased and then decreased. The activities and contents of these parameters were higher in the 0.40% and 0.60% NaCl treatments than in the 0.20% treatment and in the 0% control. <i>M. zumi</i> was the most resistant to salt stress, showing the lowest content of MDA in the leaves and roots, which increased slightly under salt stress. <i>M. baccata</i> had the highest increase in both the content and proportion of MDA. High enzyme activity was shown to play an important role in the salt resistance of <i>M. zumi</i>. Moreover, it can be speculated that there are other substances that also play a major role. We found that osmotic regulation played a key role in response to salt stress for <i>M. baccata</i> even though it was sensitive to salt stress. For <i>M. sieversii</i>, both the osmotic regulation and enzymatic antioxidants were observed to play a major role in mitigating salt stress. |
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| language | English |
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| publishDate | 2022-11-01 |
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| series | Life |
| spelling | doaj.art-466218e7b5e845b29babb9b222c3db822023-11-24T08:58:13ZengMDPI AGLife2075-17292022-11-011211192910.3390/life12111929Effects of Salt Stress on the Antioxidant Activity and Malondialdehyde, Solution Protein, Proline, and Chlorophyll Contents of Three <i>Malus</i> SpeciesDajiang Wang0Yuan Gao1Simiao Sun2Xiang Lu3Qingshan Li4Lianwen Li5Kun Wang6Jihong Liu7Xinjiang Production and Construction Corps Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization, Agricultural College of Shihezi University, Shihezi 832003, ChinaNational Repository of Apple Germplasm Resources, Key Laboratory of Horticulture Crops Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs of the People’s Republic of China, Research Institute of Pomology, Chinese Academy of Agricultural Sciences (CAAS), Xingcheng 125100, ChinaNational Repository of Apple Germplasm Resources, Key Laboratory of Horticulture Crops Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs of the People’s Republic of China, Research Institute of Pomology, Chinese Academy of Agricultural Sciences (CAAS), Xingcheng 125100, ChinaXinjiang Production and Construction Corps Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization, Agricultural College of Shihezi University, Shihezi 832003, ChinaXinjiang Production and Construction Corps Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization, Agricultural College of Shihezi University, Shihezi 832003, ChinaNational Repository of Apple Germplasm Resources, Key Laboratory of Horticulture Crops Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs of the People’s Republic of China, Research Institute of Pomology, Chinese Academy of Agricultural Sciences (CAAS), Xingcheng 125100, ChinaNational Repository of Apple Germplasm Resources, Key Laboratory of Horticulture Crops Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs of the People’s Republic of China, Research Institute of Pomology, Chinese Academy of Agricultural Sciences (CAAS), Xingcheng 125100, ChinaXinjiang Production and Construction Corps Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization, Agricultural College of Shihezi University, Shihezi 832003, ChinaUnderstanding the different physiological responses of <i>Malus</i> species under salt stress in the seedling stages will be useful in breeding salt-tolerant dwarfing apple rootstocks. Seedlings of <i>Malus Zumi</i> (Mats.) Rehd. (<i>M. zumi</i>), <i>Malus sieversii</i> (Led.) Roem. (<i>M. sieversii</i>), and <i>Malus baccata</i> (L.) Borkh. (<i>M. baccata</i>) were treated with solution of 0, 0.20%, 0.40%, and 0.60% salinity. Physiological parameters of their leaves and roots were measured at 0 d, 4 d, 8 d and 12 d after salinity treatments. Superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), malondialdehyde (MDA), solution protein (SP), and proline (PRO) initially increased and then decreased. The activities and contents of these parameters were higher in the 0.40% and 0.60% NaCl treatments than in the 0.20% treatment and in the 0% control. <i>M. zumi</i> was the most resistant to salt stress, showing the lowest content of MDA in the leaves and roots, which increased slightly under salt stress. <i>M. baccata</i> had the highest increase in both the content and proportion of MDA. High enzyme activity was shown to play an important role in the salt resistance of <i>M. zumi</i>. Moreover, it can be speculated that there are other substances that also play a major role. We found that osmotic regulation played a key role in response to salt stress for <i>M. baccata</i> even though it was sensitive to salt stress. For <i>M. sieversii</i>, both the osmotic regulation and enzymatic antioxidants were observed to play a major role in mitigating salt stress.https://www.mdpi.com/2075-1729/12/11/1929<i>Malus</i> seedlingsNaCl treatmentsenzyme activitymembrane damageosmotic regulation |
| spellingShingle | Dajiang Wang Yuan Gao Simiao Sun Xiang Lu Qingshan Li Lianwen Li Kun Wang Jihong Liu Effects of Salt Stress on the Antioxidant Activity and Malondialdehyde, Solution Protein, Proline, and Chlorophyll Contents of Three <i>Malus</i> Species Life <i>Malus</i> seedlings NaCl treatments enzyme activity membrane damage osmotic regulation |
| title | Effects of Salt Stress on the Antioxidant Activity and Malondialdehyde, Solution Protein, Proline, and Chlorophyll Contents of Three <i>Malus</i> Species |
| title_full | Effects of Salt Stress on the Antioxidant Activity and Malondialdehyde, Solution Protein, Proline, and Chlorophyll Contents of Three <i>Malus</i> Species |
| title_fullStr | Effects of Salt Stress on the Antioxidant Activity and Malondialdehyde, Solution Protein, Proline, and Chlorophyll Contents of Three <i>Malus</i> Species |
| title_full_unstemmed | Effects of Salt Stress on the Antioxidant Activity and Malondialdehyde, Solution Protein, Proline, and Chlorophyll Contents of Three <i>Malus</i> Species |
| title_short | Effects of Salt Stress on the Antioxidant Activity and Malondialdehyde, Solution Protein, Proline, and Chlorophyll Contents of Three <i>Malus</i> Species |
| title_sort | effects of salt stress on the antioxidant activity and malondialdehyde solution protein proline and chlorophyll contents of three i malus i species |
| topic | <i>Malus</i> seedlings NaCl treatments enzyme activity membrane damage osmotic regulation |
| url | https://www.mdpi.com/2075-1729/12/11/1929 |
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