A network pharmacology approach to assess Reno-protective and -curative effects of methanolic extract of Malva neglecta Wallr in gentamicin induced renal toxicity rat model
Abstract The aim of present study was to explore protective and curative effects of Malve neglecta on kidneys. In silco study with network pharmacology was performed to find out potential target organs, genes and cellular cell lines which confirmed kidneys as target organ of phyto-constituents prese...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universidade de São Paulo
2022-03-01
|
| Series: | Brazilian Journal of Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100527&tlng=en |
| _version_ | 1818353477140611072 |
|---|---|
| author | Uzma Saleem Talha Rasool Bashir Ahmad Fareeha Anwar Sana Khalid |
| author_facet | Uzma Saleem Talha Rasool Bashir Ahmad Fareeha Anwar Sana Khalid |
| author_sort | Uzma Saleem |
| collection | DOAJ |
| description | Abstract The aim of present study was to explore protective and curative effects of Malve neglecta on kidneys. In silco study with network pharmacology was performed to find out potential target organs, genes and cellular cell lines which confirmed kidneys as target organ of phyto-constituents present in Malva neglecta extract. Gentamicin (40 mg/kg, i.p) was given to induce renal toxicity. Prophylactic study was performed with 300-, 600- and 900 mg/kg doses to find out nephro-protective and -curative effects and curative potential was evaluated at 900 mg/kg dose. Renal function biomarkers, blood urea, BUN, serum creatinine and uric acid, and oxidative stress measuring biomarkers, SOD, CAT, GSH and MDA levels in kidney homogenate were quantified at the end of study. Treatment groups showed decrease in blood urea, BUN, serum creatinine and uric acid levels dose dependently and curative group also showed decline in these biomarkers. SOD, CAT, GSH levels were increased and MDA level decreased in treatment groups significantly as compared to toxic control which revealed the role of oxidative stress in renal damage and anti-oxidant power of MN. Data suggested that use of MN along with drugs causing renal toxicity may prove beneficial due to its nephro- protective and curative effects. |
| first_indexed | 2024-12-13T19:10:09Z |
| format | Article |
| id | doaj.art-466263735299446da44be4daa4681af7 |
| institution | Directory Open Access Journal |
| issn | 2175-9790 |
| language | English |
| last_indexed | 2024-12-13T19:10:09Z |
| publishDate | 2022-03-01 |
| publisher | Universidade de São Paulo |
| record_format | Article |
| series | Brazilian Journal of Pharmaceutical Sciences |
| spelling | doaj.art-466263735299446da44be4daa4681af72022-12-21T23:34:26ZengUniversidade de São PauloBrazilian Journal of Pharmaceutical Sciences2175-97902022-03-015810.1590/s2175-97902022e18965A network pharmacology approach to assess Reno-protective and -curative effects of methanolic extract of Malva neglecta Wallr in gentamicin induced renal toxicity rat modelUzma Saleemhttps://orcid.org/0000-0002-1541-4236Talha RasoolBashir AhmadFareeha AnwarSana KhalidAbstract The aim of present study was to explore protective and curative effects of Malve neglecta on kidneys. In silco study with network pharmacology was performed to find out potential target organs, genes and cellular cell lines which confirmed kidneys as target organ of phyto-constituents present in Malva neglecta extract. Gentamicin (40 mg/kg, i.p) was given to induce renal toxicity. Prophylactic study was performed with 300-, 600- and 900 mg/kg doses to find out nephro-protective and -curative effects and curative potential was evaluated at 900 mg/kg dose. Renal function biomarkers, blood urea, BUN, serum creatinine and uric acid, and oxidative stress measuring biomarkers, SOD, CAT, GSH and MDA levels in kidney homogenate were quantified at the end of study. Treatment groups showed decrease in blood urea, BUN, serum creatinine and uric acid levels dose dependently and curative group also showed decline in these biomarkers. SOD, CAT, GSH levels were increased and MDA level decreased in treatment groups significantly as compared to toxic control which revealed the role of oxidative stress in renal damage and anti-oxidant power of MN. Data suggested that use of MN along with drugs causing renal toxicity may prove beneficial due to its nephro- protective and curative effects.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100527&tlng=enMalva neglectaNetwork PharmacologyGentamicinSilymarinAnti-oxidant property |
| spellingShingle | Uzma Saleem Talha Rasool Bashir Ahmad Fareeha Anwar Sana Khalid A network pharmacology approach to assess Reno-protective and -curative effects of methanolic extract of Malva neglecta Wallr in gentamicin induced renal toxicity rat model Brazilian Journal of Pharmaceutical Sciences Malva neglecta Network Pharmacology Gentamicin Silymarin Anti-oxidant property |
| title | A network pharmacology approach to assess Reno-protective and -curative effects of methanolic extract of Malva neglecta Wallr in gentamicin induced renal toxicity rat model |
| title_full | A network pharmacology approach to assess Reno-protective and -curative effects of methanolic extract of Malva neglecta Wallr in gentamicin induced renal toxicity rat model |
| title_fullStr | A network pharmacology approach to assess Reno-protective and -curative effects of methanolic extract of Malva neglecta Wallr in gentamicin induced renal toxicity rat model |
| title_full_unstemmed | A network pharmacology approach to assess Reno-protective and -curative effects of methanolic extract of Malva neglecta Wallr in gentamicin induced renal toxicity rat model |
| title_short | A network pharmacology approach to assess Reno-protective and -curative effects of methanolic extract of Malva neglecta Wallr in gentamicin induced renal toxicity rat model |
| title_sort | network pharmacology approach to assess reno protective and curative effects of methanolic extract of malva neglecta wallr in gentamicin induced renal toxicity rat model |
| topic | Malva neglecta Network Pharmacology Gentamicin Silymarin Anti-oxidant property |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100527&tlng=en |
| work_keys_str_mv | AT uzmasaleem anetworkpharmacologyapproachtoassessrenoprotectiveandcurativeeffectsofmethanolicextractofmalvaneglectawallringentamicininducedrenaltoxicityratmodel AT talharasool anetworkpharmacologyapproachtoassessrenoprotectiveandcurativeeffectsofmethanolicextractofmalvaneglectawallringentamicininducedrenaltoxicityratmodel AT bashirahmad anetworkpharmacologyapproachtoassessrenoprotectiveandcurativeeffectsofmethanolicextractofmalvaneglectawallringentamicininducedrenaltoxicityratmodel AT fareehaanwar anetworkpharmacologyapproachtoassessrenoprotectiveandcurativeeffectsofmethanolicextractofmalvaneglectawallringentamicininducedrenaltoxicityratmodel AT sanakhalid anetworkpharmacologyapproachtoassessrenoprotectiveandcurativeeffectsofmethanolicextractofmalvaneglectawallringentamicininducedrenaltoxicityratmodel AT uzmasaleem networkpharmacologyapproachtoassessrenoprotectiveandcurativeeffectsofmethanolicextractofmalvaneglectawallringentamicininducedrenaltoxicityratmodel AT talharasool networkpharmacologyapproachtoassessrenoprotectiveandcurativeeffectsofmethanolicextractofmalvaneglectawallringentamicininducedrenaltoxicityratmodel AT bashirahmad networkpharmacologyapproachtoassessrenoprotectiveandcurativeeffectsofmethanolicextractofmalvaneglectawallringentamicininducedrenaltoxicityratmodel AT fareehaanwar networkpharmacologyapproachtoassessrenoprotectiveandcurativeeffectsofmethanolicextractofmalvaneglectawallringentamicininducedrenaltoxicityratmodel AT sanakhalid networkpharmacologyapproachtoassessrenoprotectiveandcurativeeffectsofmethanolicextractofmalvaneglectawallringentamicininducedrenaltoxicityratmodel |