The Marine-Derived Natural Product Epiloliolide Isolated from <i>Sargassum horneri</i> Regulates NLRP3 via PKA/CREB, Promoting Proliferation and Anti-Inflammatory Effects of Human Periodontal Ligament Cells
Currently, periodontitis treatment relies on surgical operations, anti-inflammatory agents, or antibiotics. However, these treatments cause pain and side effects, resulting in a poor prognosis. Therefore, in this study, we evaluated the impact of the compound epiloliolide isolated from <i>Sarg...
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MDPI AG
2021-07-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/19/7/388 |
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| author | Eun-Nam Kim Woguti Yvonne Nabende Hyeyoon Jeong Dongyup Hahn Gil-Saeng Jeong |
| author_facet | Eun-Nam Kim Woguti Yvonne Nabende Hyeyoon Jeong Dongyup Hahn Gil-Saeng Jeong |
| author_sort | Eun-Nam Kim |
| collection | DOAJ |
| description | Currently, periodontitis treatment relies on surgical operations, anti-inflammatory agents, or antibiotics. However, these treatments cause pain and side effects, resulting in a poor prognosis. Therefore, in this study, we evaluated the impact of the compound epiloliolide isolated from <i>Sargassum horneri</i> on the recovery of inflammatory inhibitors and loss of periodontal ligaments, which are essential treatment strategies for periodontitis. Here, human periodontal ligament cells stimulated with PG-LPS were treated with the compound epiloliolide, isolated from <i>S. horneri.</i> In the results of this study, epiloliolide proved the anti-inflammatory effect, cell proliferation capacity, and differentiation potential of periodontal ligament cells into osteoblasts, through the regulation of the PKA/CREB signaling pathway. Epiloliolide effectively increased the proliferation and migration of human periodontal ligament cells without cytotoxicity and suppressed the protein expression of proinflammatory mediators and cytokines, such as iNOS, COX-2, TNF-α, IL-6, and IL-1β, by downregulating NLRP3 activated by PG-LPS. Epiloliolide also upregulated the phosphorylation of PKA/CREB proteins, which play an important role in cell growth and proliferation. It was confirmed that the anti-inflammatory effect in PG-LPS-stimulated large cells was due to the regulation of PKA/CREB signaling. We suggest that epiloliolide could serve as a potential novel therapeutic agent for periodontitis by inhibiting inflammation and restoring the loss of periodontal tissue. |
| first_indexed | 2024-03-10T09:34:14Z |
| format | Article |
| id | doaj.art-46635734708641d6873c35bd86b4ff4d |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-10T09:34:14Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-46635734708641d6873c35bd86b4ff4d2023-11-22T04:14:12ZengMDPI AGMarine Drugs1660-33972021-07-0119738810.3390/md19070388The Marine-Derived Natural Product Epiloliolide Isolated from <i>Sargassum horneri</i> Regulates NLRP3 via PKA/CREB, Promoting Proliferation and Anti-Inflammatory Effects of Human Periodontal Ligament CellsEun-Nam Kim0Woguti Yvonne Nabende1Hyeyoon Jeong2Dongyup Hahn3Gil-Saeng Jeong4College of Pharmacy, Keimyung University, 1095 Dalgubeol-daero, Daegu 42601, KoreaSchool of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kyungpook National University, Daegu 41566, KoreaSchool of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kyungpook National University, Daegu 41566, KoreaSchool of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kyungpook National University, Daegu 41566, KoreaCollege of Pharmacy, Keimyung University, 1095 Dalgubeol-daero, Daegu 42601, KoreaCurrently, periodontitis treatment relies on surgical operations, anti-inflammatory agents, or antibiotics. However, these treatments cause pain and side effects, resulting in a poor prognosis. Therefore, in this study, we evaluated the impact of the compound epiloliolide isolated from <i>Sargassum horneri</i> on the recovery of inflammatory inhibitors and loss of periodontal ligaments, which are essential treatment strategies for periodontitis. Here, human periodontal ligament cells stimulated with PG-LPS were treated with the compound epiloliolide, isolated from <i>S. horneri.</i> In the results of this study, epiloliolide proved the anti-inflammatory effect, cell proliferation capacity, and differentiation potential of periodontal ligament cells into osteoblasts, through the regulation of the PKA/CREB signaling pathway. Epiloliolide effectively increased the proliferation and migration of human periodontal ligament cells without cytotoxicity and suppressed the protein expression of proinflammatory mediators and cytokines, such as iNOS, COX-2, TNF-α, IL-6, and IL-1β, by downregulating NLRP3 activated by PG-LPS. Epiloliolide also upregulated the phosphorylation of PKA/CREB proteins, which play an important role in cell growth and proliferation. It was confirmed that the anti-inflammatory effect in PG-LPS-stimulated large cells was due to the regulation of PKA/CREB signaling. We suggest that epiloliolide could serve as a potential novel therapeutic agent for periodontitis by inhibiting inflammation and restoring the loss of periodontal tissue.https://www.mdpi.com/1660-3397/19/7/388<i>Sargassum horneri</i>periodontitisprotein kinase A (PKA)cAMP-responsive element-binding protein (CREB)epiloliolide |
| spellingShingle | Eun-Nam Kim Woguti Yvonne Nabende Hyeyoon Jeong Dongyup Hahn Gil-Saeng Jeong The Marine-Derived Natural Product Epiloliolide Isolated from <i>Sargassum horneri</i> Regulates NLRP3 via PKA/CREB, Promoting Proliferation and Anti-Inflammatory Effects of Human Periodontal Ligament Cells Marine Drugs <i>Sargassum horneri</i> periodontitis protein kinase A (PKA) cAMP-responsive element-binding protein (CREB) epiloliolide |
| title | The Marine-Derived Natural Product Epiloliolide Isolated from <i>Sargassum horneri</i> Regulates NLRP3 via PKA/CREB, Promoting Proliferation and Anti-Inflammatory Effects of Human Periodontal Ligament Cells |
| title_full | The Marine-Derived Natural Product Epiloliolide Isolated from <i>Sargassum horneri</i> Regulates NLRP3 via PKA/CREB, Promoting Proliferation and Anti-Inflammatory Effects of Human Periodontal Ligament Cells |
| title_fullStr | The Marine-Derived Natural Product Epiloliolide Isolated from <i>Sargassum horneri</i> Regulates NLRP3 via PKA/CREB, Promoting Proliferation and Anti-Inflammatory Effects of Human Periodontal Ligament Cells |
| title_full_unstemmed | The Marine-Derived Natural Product Epiloliolide Isolated from <i>Sargassum horneri</i> Regulates NLRP3 via PKA/CREB, Promoting Proliferation and Anti-Inflammatory Effects of Human Periodontal Ligament Cells |
| title_short | The Marine-Derived Natural Product Epiloliolide Isolated from <i>Sargassum horneri</i> Regulates NLRP3 via PKA/CREB, Promoting Proliferation and Anti-Inflammatory Effects of Human Periodontal Ligament Cells |
| title_sort | marine derived natural product epiloliolide isolated from i sargassum horneri i regulates nlrp3 via pka creb promoting proliferation and anti inflammatory effects of human periodontal ligament cells |
| topic | <i>Sargassum horneri</i> periodontitis protein kinase A (PKA) cAMP-responsive element-binding protein (CREB) epiloliolide |
| url | https://www.mdpi.com/1660-3397/19/7/388 |
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