Relationship between Hormonal Modulation and Gastroprotective Activity of Malvidin and Cyanidin Chloride: In Vivo and In Silico Approach
Peptic ulcers are lesions that affect the gastrointestinal tract and that can be triggered by external factors such as alcohol use. This study investigated the gastroprotective role of two anthocyanidins, malvidin and cyanidin chloride, in an ethanol-induced gastric ulcer model in male and female mi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/14/3/565 |
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| author | Melina Luzzi Zarricueta Felipe Leonardo Fagundes Quélita Cristina Pereira Simone Queiroz Pantaleão Raquel de Cássia dos Santos |
| author_facet | Melina Luzzi Zarricueta Felipe Leonardo Fagundes Quélita Cristina Pereira Simone Queiroz Pantaleão Raquel de Cássia dos Santos |
| author_sort | Melina Luzzi Zarricueta |
| collection | DOAJ |
| description | Peptic ulcers are lesions that affect the gastrointestinal tract and that can be triggered by external factors such as alcohol use. This study investigated the gastroprotective role of two anthocyanidins, malvidin and cyanidin chloride, in an ethanol-induced gastric ulcer model in male and female mice (ovariectomized and supplemented with 17β-estradiol or not) and aimed to evaluate the effectiveness of anthocyanidins in preventing the formation of lesions and to identify the underlying mechanisms, while considering hormonal differences. Moreover, in silico comparative analysis was performed to predict the properties and biological behaviors of the molecules. We observed that the hormonal status did not interfere with the gastroprotective action of malvidin, although antioxidant mechanisms were modulated differently depending on sex. On the other hand, cyanidin showed gastroprotective activity at different doses, demonstrating that, for the same experimental model, there is a need to adjust the effective dose depending on sex. In silico analysis showed that, despite being structurally similar, the interaction with receptors and target proteins in this study (myeloperoxidase, superoxide dismutase, catalase, and reduced glutathione) differed between the two molecules, which explains the difference observed in in vivo treatments. |
| first_indexed | 2024-03-09T12:58:40Z |
| format | Article |
| id | doaj.art-46651c1ab68741bd8aa100f94c8b3e8b |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-09T12:58:40Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-46651c1ab68741bd8aa100f94c8b3e8b2023-11-30T21:56:41ZengMDPI AGPharmaceutics1999-49232022-03-0114356510.3390/pharmaceutics14030565Relationship between Hormonal Modulation and Gastroprotective Activity of Malvidin and Cyanidin Chloride: In Vivo and In Silico ApproachMelina Luzzi Zarricueta0Felipe Leonardo Fagundes1Quélita Cristina Pereira2Simone Queiroz Pantaleão3Raquel de Cássia dos Santos4Laboratory of Pharmacology and Molecular Biology, Post Graduate Program in Health Sciences, Medical School, São Francisco University, Bragança Paulista, São Paulo 12916-900, BrazilLaboratory of Pharmacology and Molecular Biology, Post Graduate Program in Health Sciences, Medical School, São Francisco University, Bragança Paulista, São Paulo 12916-900, BrazilLaboratory of Pharmacology and Molecular Biology, Post Graduate Program in Health Sciences, Medical School, São Francisco University, Bragança Paulista, São Paulo 12916-900, BrazilLaboratory of Computational Biology and Bioinformatics, Federal University of ABC, Av. dos Estados, 5001–Bangú, Santo André 09210-580, BrazilLaboratory of Pharmacology and Molecular Biology, Post Graduate Program in Health Sciences, Medical School, São Francisco University, Bragança Paulista, São Paulo 12916-900, BrazilPeptic ulcers are lesions that affect the gastrointestinal tract and that can be triggered by external factors such as alcohol use. This study investigated the gastroprotective role of two anthocyanidins, malvidin and cyanidin chloride, in an ethanol-induced gastric ulcer model in male and female mice (ovariectomized and supplemented with 17β-estradiol or not) and aimed to evaluate the effectiveness of anthocyanidins in preventing the formation of lesions and to identify the underlying mechanisms, while considering hormonal differences. Moreover, in silico comparative analysis was performed to predict the properties and biological behaviors of the molecules. We observed that the hormonal status did not interfere with the gastroprotective action of malvidin, although antioxidant mechanisms were modulated differently depending on sex. On the other hand, cyanidin showed gastroprotective activity at different doses, demonstrating that, for the same experimental model, there is a need to adjust the effective dose depending on sex. In silico analysis showed that, despite being structurally similar, the interaction with receptors and target proteins in this study (myeloperoxidase, superoxide dismutase, catalase, and reduced glutathione) differed between the two molecules, which explains the difference observed in in vivo treatments.https://www.mdpi.com/1999-4923/14/3/565ethanol-induced gastric lesionanthocyanidinssex differences |
| spellingShingle | Melina Luzzi Zarricueta Felipe Leonardo Fagundes Quélita Cristina Pereira Simone Queiroz Pantaleão Raquel de Cássia dos Santos Relationship between Hormonal Modulation and Gastroprotective Activity of Malvidin and Cyanidin Chloride: In Vivo and In Silico Approach Pharmaceutics ethanol-induced gastric lesion anthocyanidins sex differences |
| title | Relationship between Hormonal Modulation and Gastroprotective Activity of Malvidin and Cyanidin Chloride: In Vivo and In Silico Approach |
| title_full | Relationship between Hormonal Modulation and Gastroprotective Activity of Malvidin and Cyanidin Chloride: In Vivo and In Silico Approach |
| title_fullStr | Relationship between Hormonal Modulation and Gastroprotective Activity of Malvidin and Cyanidin Chloride: In Vivo and In Silico Approach |
| title_full_unstemmed | Relationship between Hormonal Modulation and Gastroprotective Activity of Malvidin and Cyanidin Chloride: In Vivo and In Silico Approach |
| title_short | Relationship between Hormonal Modulation and Gastroprotective Activity of Malvidin and Cyanidin Chloride: In Vivo and In Silico Approach |
| title_sort | relationship between hormonal modulation and gastroprotective activity of malvidin and cyanidin chloride in vivo and in silico approach |
| topic | ethanol-induced gastric lesion anthocyanidins sex differences |
| url | https://www.mdpi.com/1999-4923/14/3/565 |
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