Comparison of antibody titers using conventional tube technique versus column agglutination technique in ABO blood group incompatible renal transplant
Introduction: Measurement of alloantibody titer to a red cell antigen (ABO titers) is an integral part of management of ABO incompatible kidney transplants (ABOiKT). Material and Methods: There are different methods of titer estimation. Alloantibody detection by tube titration and Gel agglutination...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2017-01-01
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| Series: | Asian Journal of Transfusion Science |
| Subjects: | |
| Online Access: | http://www.ajts.org/article.asp?issn=0973-6247;year=2017;volume=11;issue=2;spage=131;epage=134;aulast=Bhangale |
| _version_ | 1818840606104879104 |
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| author | Amit Bhangale Amardeep Pathak Smita Pawar Tarun Jeloka |
| author_facet | Amit Bhangale Amardeep Pathak Smita Pawar Tarun Jeloka |
| author_sort | Amit Bhangale |
| collection | DOAJ |
| description | Introduction: Measurement of alloantibody titer to a red cell antigen (ABO titers) is an integral part of management of ABO incompatible kidney transplants (ABOiKT).
Material and Methods: There are different methods of titer estimation. Alloantibody detection by tube titration and Gel agglutination columns are accepted methodologies. It is essential to find the difference in titers between the two methods so as to set the 'cut-off' titer accordingly, depending upon the method used.
Results: We did a prospective observational study to compare and correlate the ABO titers using these two different techniques – conventional tube technique (CTT) and the newer column agglutination technique (CAT). A total of 67 samples were processed in parallel for anti-A/B antibodies by both tube dilution and column agglutination methods. The mean titer by conventional tube method was 38.5 + 96.6 and by the column agglutination test was 96.4 + 225. The samples correlated well with Spearman rho correlation coefficient of 0.94 (P = 0.01).
Conclusion: The column agglutination method for anti A/B titer estimation in an ABO incompatible kidney transplant is more sensitive, with the column agglutination results being approximately two and half fold higher (one more dilution) than that of tube method. |
| first_indexed | 2024-12-19T04:12:51Z |
| format | Article |
| id | doaj.art-4667b8b30fc9457e803cf86cb2055df9 |
| institution | Directory Open Access Journal |
| issn | 0973-6247 1998-3565 |
| language | English |
| last_indexed | 2024-12-19T04:12:51Z |
| publishDate | 2017-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Journal of Transfusion Science |
| spelling | doaj.art-4667b8b30fc9457e803cf86cb2055df92022-12-21T20:36:22ZengWolters Kluwer Medknow PublicationsAsian Journal of Transfusion Science0973-62471998-35652017-01-0111213113410.4103/0973-6247.214343Comparison of antibody titers using conventional tube technique versus column agglutination technique in ABO blood group incompatible renal transplantAmit BhangaleAmardeep PathakSmita PawarTarun JelokaIntroduction: Measurement of alloantibody titer to a red cell antigen (ABO titers) is an integral part of management of ABO incompatible kidney transplants (ABOiKT). Material and Methods: There are different methods of titer estimation. Alloantibody detection by tube titration and Gel agglutination columns are accepted methodologies. It is essential to find the difference in titers between the two methods so as to set the 'cut-off' titer accordingly, depending upon the method used. Results: We did a prospective observational study to compare and correlate the ABO titers using these two different techniques – conventional tube technique (CTT) and the newer column agglutination technique (CAT). A total of 67 samples were processed in parallel for anti-A/B antibodies by both tube dilution and column agglutination methods. The mean titer by conventional tube method was 38.5 + 96.6 and by the column agglutination test was 96.4 + 225. The samples correlated well with Spearman rho correlation coefficient of 0.94 (P = 0.01). Conclusion: The column agglutination method for anti A/B titer estimation in an ABO incompatible kidney transplant is more sensitive, with the column agglutination results being approximately two and half fold higher (one more dilution) than that of tube method.http://www.ajts.org/article.asp?issn=0973-6247;year=2017;volume=11;issue=2;spage=131;epage=134;aulast=BhangaleABO incompatible kidney transplantscolumn agglutination techniqueconventional tube technique |
| spellingShingle | Amit Bhangale Amardeep Pathak Smita Pawar Tarun Jeloka Comparison of antibody titers using conventional tube technique versus column agglutination technique in ABO blood group incompatible renal transplant Asian Journal of Transfusion Science ABO incompatible kidney transplants column agglutination technique conventional tube technique |
| title | Comparison of antibody titers using conventional tube technique versus column agglutination technique in ABO blood group incompatible renal transplant |
| title_full | Comparison of antibody titers using conventional tube technique versus column agglutination technique in ABO blood group incompatible renal transplant |
| title_fullStr | Comparison of antibody titers using conventional tube technique versus column agglutination technique in ABO blood group incompatible renal transplant |
| title_full_unstemmed | Comparison of antibody titers using conventional tube technique versus column agglutination technique in ABO blood group incompatible renal transplant |
| title_short | Comparison of antibody titers using conventional tube technique versus column agglutination technique in ABO blood group incompatible renal transplant |
| title_sort | comparison of antibody titers using conventional tube technique versus column agglutination technique in abo blood group incompatible renal transplant |
| topic | ABO incompatible kidney transplants column agglutination technique conventional tube technique |
| url | http://www.ajts.org/article.asp?issn=0973-6247;year=2017;volume=11;issue=2;spage=131;epage=134;aulast=Bhangale |
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