Effect of silica-coated magnetic nanoparticles on rigidity sensing of human embryonic kidney cells
Abstract Background Nanoparticles (NPs) can enter cells and cause cellular dysfunction. For example, reactive oxygen species generated by NPs can damage the cytoskeleton and impair cellular adhesion properties. Previously, we reported that cell spreading and protrusion structures such as lamellipodi...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2020-11-01
|
| Series: | Journal of Nanobiotechnology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12951-020-00730-2 |
| _version_ | 1797999258833518592 |
|---|---|
| author | Abdurazak Aman Ketebo Tae Hwan Shin Myeongjun Jun Gwang Lee Sungsu Park |
| author_facet | Abdurazak Aman Ketebo Tae Hwan Shin Myeongjun Jun Gwang Lee Sungsu Park |
| author_sort | Abdurazak Aman Ketebo |
| collection | DOAJ |
| description | Abstract Background Nanoparticles (NPs) can enter cells and cause cellular dysfunction. For example, reactive oxygen species generated by NPs can damage the cytoskeleton and impair cellular adhesion properties. Previously, we reported that cell spreading and protrusion structures such as lamellipodia and filopodia was reduced when cells are treated with silica-coated magnetic nanoparticles incorporating rhodamine B isothiocyanate (MNPs@SiO2(RITC)), even at 0.1 μg/μL. These protruded structures are involved in a cell’s rigidity sensing, but how these NPs affect rigidity sensing is unknown. Results Here, we report that the rigidity sensing of human embryonic kidney (HEK293) cells was impaired even at 0.1 μg/μL of MNPs@SiO2(RITC). At this concentration, cells were unable to discern the stiffness difference between soft (5 kPa) and rigid (2 MPa) flat surfaces. The impairment of rigidity sensing was further supported by observing the disappearance of locally contracted elastomeric submicron pillars (900 nm in diameter, 2 μm in height, 24.21 nN/μm in stiffness k) under MNPs@SiO2(RITC) treated cells. A decrease in the phosphorylation of paxillin, which is involved in focal adhesion dynamics, may cause cells to be insensitive to stiffness differences when they are treated with MNPs@SiO2(RITC). Conclusions Our results suggest that NPs may impair the rigidity sensing of cells even at low concentrations, thereby affecting cell adhesion and spreading. |
| first_indexed | 2024-04-11T11:00:41Z |
| format | Article |
| id | doaj.art-466b8516f6be4400a8c9b8a5b0349804 |
| institution | Directory Open Access Journal |
| issn | 1477-3155 |
| language | English |
| last_indexed | 2024-04-11T11:00:41Z |
| publishDate | 2020-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj.art-466b8516f6be4400a8c9b8a5b03498042022-12-22T04:28:36ZengBMCJournal of Nanobiotechnology1477-31552020-11-0118111110.1186/s12951-020-00730-2Effect of silica-coated magnetic nanoparticles on rigidity sensing of human embryonic kidney cellsAbdurazak Aman Ketebo0Tae Hwan Shin1Myeongjun Jun2Gwang Lee3Sungsu Park4School of Mechanical Engineering, Sungkyunkwan UniversityDepartment of Physiology, Ajou University School of MedicineSchool of Mechanical Engineering, Sungkyunkwan UniversityDepartment of Physiology, Ajou University School of MedicineSchool of Mechanical Engineering, Sungkyunkwan UniversityAbstract Background Nanoparticles (NPs) can enter cells and cause cellular dysfunction. For example, reactive oxygen species generated by NPs can damage the cytoskeleton and impair cellular adhesion properties. Previously, we reported that cell spreading and protrusion structures such as lamellipodia and filopodia was reduced when cells are treated with silica-coated magnetic nanoparticles incorporating rhodamine B isothiocyanate (MNPs@SiO2(RITC)), even at 0.1 μg/μL. These protruded structures are involved in a cell’s rigidity sensing, but how these NPs affect rigidity sensing is unknown. Results Here, we report that the rigidity sensing of human embryonic kidney (HEK293) cells was impaired even at 0.1 μg/μL of MNPs@SiO2(RITC). At this concentration, cells were unable to discern the stiffness difference between soft (5 kPa) and rigid (2 MPa) flat surfaces. The impairment of rigidity sensing was further supported by observing the disappearance of locally contracted elastomeric submicron pillars (900 nm in diameter, 2 μm in height, 24.21 nN/μm in stiffness k) under MNPs@SiO2(RITC) treated cells. A decrease in the phosphorylation of paxillin, which is involved in focal adhesion dynamics, may cause cells to be insensitive to stiffness differences when they are treated with MNPs@SiO2(RITC). Conclusions Our results suggest that NPs may impair the rigidity sensing of cells even at low concentrations, thereby affecting cell adhesion and spreading.http://link.springer.com/article/10.1186/s12951-020-00730-2LamellipodiaFilopodiaRigidity sensingSilica-coated magnetic nanoparticlesTraction force |
| spellingShingle | Abdurazak Aman Ketebo Tae Hwan Shin Myeongjun Jun Gwang Lee Sungsu Park Effect of silica-coated magnetic nanoparticles on rigidity sensing of human embryonic kidney cells Journal of Nanobiotechnology Lamellipodia Filopodia Rigidity sensing Silica-coated magnetic nanoparticles Traction force |
| title | Effect of silica-coated magnetic nanoparticles on rigidity sensing of human embryonic kidney cells |
| title_full | Effect of silica-coated magnetic nanoparticles on rigidity sensing of human embryonic kidney cells |
| title_fullStr | Effect of silica-coated magnetic nanoparticles on rigidity sensing of human embryonic kidney cells |
| title_full_unstemmed | Effect of silica-coated magnetic nanoparticles on rigidity sensing of human embryonic kidney cells |
| title_short | Effect of silica-coated magnetic nanoparticles on rigidity sensing of human embryonic kidney cells |
| title_sort | effect of silica coated magnetic nanoparticles on rigidity sensing of human embryonic kidney cells |
| topic | Lamellipodia Filopodia Rigidity sensing Silica-coated magnetic nanoparticles Traction force |
| url | http://link.springer.com/article/10.1186/s12951-020-00730-2 |
| work_keys_str_mv | AT abdurazakamanketebo effectofsilicacoatedmagneticnanoparticlesonrigiditysensingofhumanembryonickidneycells AT taehwanshin effectofsilicacoatedmagneticnanoparticlesonrigiditysensingofhumanembryonickidneycells AT myeongjunjun effectofsilicacoatedmagneticnanoparticlesonrigiditysensingofhumanembryonickidneycells AT gwanglee effectofsilicacoatedmagneticnanoparticlesonrigiditysensingofhumanembryonickidneycells AT sungsupark effectofsilicacoatedmagneticnanoparticlesonrigiditysensingofhumanembryonickidneycells |