A Horizontal Magnetic Tweezers for Studying Single DNA Molecules and DNA-Binding Proteins
We report data from single molecule studies on the interaction between single DNA molecules and core histones using custom-designed horizontal magnetic tweezers. The DNA-core histone complexes were formed using λ-DNA tethers, core histones, and NAP1 and were exposed to forces ranging from ~2 pN to ~...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-08-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/16/4781 |
| _version_ | 1797522693025693696 |
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| author | Roberto Fabian Santosh Gaire Christopher Tyson Raghabendra Adhikari Ian Pegg Abhijit Sarkar |
| author_facet | Roberto Fabian Santosh Gaire Christopher Tyson Raghabendra Adhikari Ian Pegg Abhijit Sarkar |
| author_sort | Roberto Fabian |
| collection | DOAJ |
| description | We report data from single molecule studies on the interaction between single DNA molecules and core histones using custom-designed horizontal magnetic tweezers. The DNA-core histone complexes were formed using λ-DNA tethers, core histones, and NAP1 and were exposed to forces ranging from ~2 pN to ~74 pN. During the assembly events, we observed the length of the DNA decrease in approximate integer multiples of ~50 nm, suggesting the binding of the histone octamers to the DNA tether. During the mechanically induced disassembly events, we observed disruption lengths in approximate integer multiples of ~50 nm, suggesting the unbinding of one or more octamers from the DNA tether. We also observed histone octamer unbinding events at forces as low as ~2 pN. Our horizontal magnetic tweezers yielded high-resolution, low-noise data on force-mediated DNA-core histone assembly and disassembly processes. |
| first_indexed | 2024-03-10T08:33:02Z |
| format | Article |
| id | doaj.art-466de3cd36e14ff89bef7dd0b84e00c8 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T08:33:02Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-466de3cd36e14ff89bef7dd0b84e00c82023-11-22T08:51:52ZengMDPI AGMolecules1420-30492021-08-012616478110.3390/molecules26164781A Horizontal Magnetic Tweezers for Studying Single DNA Molecules and DNA-Binding ProteinsRoberto Fabian0Santosh Gaire1Christopher Tyson2Raghabendra Adhikari3Ian Pegg4Abhijit Sarkar5Department of Physics and Vitreous State Laboratory, The Catholic University of America, Washington, DC 20064, USADepartment of Physics and Vitreous State Laboratory, The Catholic University of America, Washington, DC 20064, USABiomedical Engineering Department and Vitreous State Laboratory, The Catholic University of America, Washington, DC 20064, USADepartment of Biology, The Catholic University of America, Washington, DC 20064, USADepartment of Physics and Vitreous State Laboratory, The Catholic University of America, Washington, DC 20064, USADepartment of Physics and Vitreous State Laboratory, The Catholic University of America, Washington, DC 20064, USAWe report data from single molecule studies on the interaction between single DNA molecules and core histones using custom-designed horizontal magnetic tweezers. The DNA-core histone complexes were formed using λ-DNA tethers, core histones, and NAP1 and were exposed to forces ranging from ~2 pN to ~74 pN. During the assembly events, we observed the length of the DNA decrease in approximate integer multiples of ~50 nm, suggesting the binding of the histone octamers to the DNA tether. During the mechanically induced disassembly events, we observed disruption lengths in approximate integer multiples of ~50 nm, suggesting the unbinding of one or more octamers from the DNA tether. We also observed histone octamer unbinding events at forces as low as ~2 pN. Our horizontal magnetic tweezers yielded high-resolution, low-noise data on force-mediated DNA-core histone assembly and disassembly processes.https://www.mdpi.com/1420-3049/26/16/4781single molecule micromanipulation techniqueshorizontal magnetic tweezersDNA-histone interactionsnucleosomes |
| spellingShingle | Roberto Fabian Santosh Gaire Christopher Tyson Raghabendra Adhikari Ian Pegg Abhijit Sarkar A Horizontal Magnetic Tweezers for Studying Single DNA Molecules and DNA-Binding Proteins Molecules single molecule micromanipulation techniques horizontal magnetic tweezers DNA-histone interactions nucleosomes |
| title | A Horizontal Magnetic Tweezers for Studying Single DNA Molecules and DNA-Binding Proteins |
| title_full | A Horizontal Magnetic Tweezers for Studying Single DNA Molecules and DNA-Binding Proteins |
| title_fullStr | A Horizontal Magnetic Tweezers for Studying Single DNA Molecules and DNA-Binding Proteins |
| title_full_unstemmed | A Horizontal Magnetic Tweezers for Studying Single DNA Molecules and DNA-Binding Proteins |
| title_short | A Horizontal Magnetic Tweezers for Studying Single DNA Molecules and DNA-Binding Proteins |
| title_sort | horizontal magnetic tweezers for studying single dna molecules and dna binding proteins |
| topic | single molecule micromanipulation techniques horizontal magnetic tweezers DNA-histone interactions nucleosomes |
| url | https://www.mdpi.com/1420-3049/26/16/4781 |
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