A rapid sampling technique for isolating highly productive lipid-rich algae strains from environmental samples
Strain selection and isolation of lipid-rich microalgae are among the two most important steps for screening isolates with maximum biofuel productivity. In this work, we introduce a novel direct sampling technique that allows native strains to be selected for rapid growth under defined conditions fo...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Alpha Creation Enterprise
2019-03-01
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| Series: | Biofuel Research Journal |
| Subjects: | |
| Online Access: | http://www.biofueljournal.com/article_82393_5dde1a56b5afdffee0c6289d8f8b32bd.pdf |
| Summary: | Strain selection and isolation of lipid-rich microalgae are among the two most important steps for screening isolates with maximum biofuel productivity. In this work, we introduce a novel direct sampling technique that allows native strains to be selected for rapid growth under defined conditions followed by direct selection of product-rich species, two desirable characteristics of algae for mass culture. This sampling strategy directly selects the lipid-rich strains visualized under an inverted fluorescence microscope using an X-Y-Z micromanipulator. The enrichment step can be manipulated to select for strains with specific technological applications. Direct sampling of lipid-rich cells avoids the tedious task of screening isolates while using relatively inexpensive equipment. |
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| ISSN: | 2292-8782 2292-8782 |