Detection and differentiation of Entamoeba histolytica and Entamoeba dispar in clinical samples through PCR-denaturing gradient gel electrophoresis
Amebiasis is one of the twenty major causes of disease in Mexico; however, the diagnosis is difficult due to limitations of conventional microscopy-based techniques. In this study, we analyzed stool samples using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) to differe...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
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| Series: | Brazilian Journal of Medical and Biological Research |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000400607&lng=en&tlng=en |
| _version_ | 1819140401907367936 |
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| author | P. López-López M.C. Martínez-López X.M. Boldo-León Y. Hernández-Díaz T.B. González-Castro C.A. Tovilla-Zárate J.P. Luna-Arias |
| author_facet | P. López-López M.C. Martínez-López X.M. Boldo-León Y. Hernández-Díaz T.B. González-Castro C.A. Tovilla-Zárate J.P. Luna-Arias |
| author_sort | P. López-López |
| collection | DOAJ |
| description | Amebiasis is one of the twenty major causes of disease in Mexico; however, the diagnosis is difficult due to limitations of conventional microscopy-based techniques. In this study, we analyzed stool samples using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) to differentiate between Entamoeba histolytica (pathogenic) and E. dispar (non-pathogenic). The target for the PCR amplification was a small region (228 bp) of the adh112 gene selected to increase the sensitivity of the test. The study involved 62 stool samples that were collected from individuals with complaints of gastrointestinal discomfort. Of the 62 samples, 10 (16.1%) were positive for E. histolytica while 52 (83.9%) were negative. No sample was positive for E. dispar. These results were validated by nested PCR-RFLP (restriction fragment length polymorphism) and suggest that PCR-DGGE is a promising tool to differentiate among Entamoeba infections, contributing to determine the specific treatment for patients infected with E. histolytica, and therefore, avoiding unnecessary treatment of patients infected with the non-pathogenic E. dispar. |
| first_indexed | 2024-12-22T11:37:59Z |
| format | Article |
| id | doaj.art-46732d15848b499dabcd377577f11897 |
| institution | Directory Open Access Journal |
| issn | 1414-431X |
| language | English |
| last_indexed | 2024-12-22T11:37:59Z |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-46732d15848b499dabcd377577f118972022-12-21T18:27:22ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research1414-431X50410.1590/1414-431x20175997S0100-879X2017000400607Detection and differentiation of Entamoeba histolytica and Entamoeba dispar in clinical samples through PCR-denaturing gradient gel electrophoresisP. López-LópezM.C. Martínez-LópezX.M. Boldo-LeónY. Hernández-DíazT.B. González-CastroC.A. Tovilla-ZárateJ.P. Luna-AriasAmebiasis is one of the twenty major causes of disease in Mexico; however, the diagnosis is difficult due to limitations of conventional microscopy-based techniques. In this study, we analyzed stool samples using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) to differentiate between Entamoeba histolytica (pathogenic) and E. dispar (non-pathogenic). The target for the PCR amplification was a small region (228 bp) of the adh112 gene selected to increase the sensitivity of the test. The study involved 62 stool samples that were collected from individuals with complaints of gastrointestinal discomfort. Of the 62 samples, 10 (16.1%) were positive for E. histolytica while 52 (83.9%) were negative. No sample was positive for E. dispar. These results were validated by nested PCR-RFLP (restriction fragment length polymorphism) and suggest that PCR-DGGE is a promising tool to differentiate among Entamoeba infections, contributing to determine the specific treatment for patients infected with E. histolytica, and therefore, avoiding unnecessary treatment of patients infected with the non-pathogenic E. dispar.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000400607&lng=en&tlng=enAmebiasisNeglected diseasesDiagnosticPCRDGGEadh112 gene |
| spellingShingle | P. López-López M.C. Martínez-López X.M. Boldo-León Y. Hernández-Díaz T.B. González-Castro C.A. Tovilla-Zárate J.P. Luna-Arias Detection and differentiation of Entamoeba histolytica and Entamoeba dispar in clinical samples through PCR-denaturing gradient gel electrophoresis Brazilian Journal of Medical and Biological Research Amebiasis Neglected diseases Diagnostic PCR DGGE adh112 gene |
| title | Detection and differentiation of Entamoeba histolytica and Entamoeba dispar in clinical samples through PCR-denaturing gradient gel electrophoresis |
| title_full | Detection and differentiation of Entamoeba histolytica and Entamoeba dispar in clinical samples through PCR-denaturing gradient gel electrophoresis |
| title_fullStr | Detection and differentiation of Entamoeba histolytica and Entamoeba dispar in clinical samples through PCR-denaturing gradient gel electrophoresis |
| title_full_unstemmed | Detection and differentiation of Entamoeba histolytica and Entamoeba dispar in clinical samples through PCR-denaturing gradient gel electrophoresis |
| title_short | Detection and differentiation of Entamoeba histolytica and Entamoeba dispar in clinical samples through PCR-denaturing gradient gel electrophoresis |
| title_sort | detection and differentiation of entamoeba histolytica and entamoeba dispar in clinical samples through pcr denaturing gradient gel electrophoresis |
| topic | Amebiasis Neglected diseases Diagnostic PCR DGGE adh112 gene |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000400607&lng=en&tlng=en |
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