Protocol for electron microscopy of Drosophila photoreceptor cells
Summary: In Drosophila, mutations in genes that prevent normal Ca2+ influx after light stimulation usually cause light-dependent retinal degeneration or neurodegeneration, detectable by defects in eye morphology. Here, we present a protocol based on electron microscopy (EM) to observe the morphologi...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-09-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722003768 |
| _version_ | 1811333620692942848 |
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| author | Qiuxiang Gu Yao Tian Junhai Han |
| author_facet | Qiuxiang Gu Yao Tian Junhai Han |
| author_sort | Qiuxiang Gu |
| collection | DOAJ |
| description | Summary: In Drosophila, mutations in genes that prevent normal Ca2+ influx after light stimulation usually cause light-dependent retinal degeneration or neurodegeneration, detectable by defects in eye morphology. Here, we present a protocol based on electron microscopy (EM) to observe the morphological structure of photoreceptor cells in Drosophila. We detail how to fix, dehydrate, embed, and polymerize compound eye samples, followed by sectioning, post-staining, and image acquisition, to assess the eye morphology at the ultrastructural level.For complete details on the use and execution of this protocol, please refer to Gu et al. (2020). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-13T16:55:37Z |
| format | Article |
| id | doaj.art-46737318c00a470ebbd7dbced9954340 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-13T16:55:37Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-46737318c00a470ebbd7dbced99543402022-12-22T02:38:49ZengElsevierSTAR Protocols2666-16672022-09-0133101496Protocol for electron microscopy of Drosophila photoreceptor cellsQiuxiang Gu0Yao Tian1Junhai Han2School of Life Science, Nantong University, Nantong, Jiangsu 226001, ChinaSchool of Life Science and Technology, the Key Laboratory of Developmental Genes and Human Disease, Southeast University, Nanjing 210096, China; Corresponding authorSchool of Life Science and Technology, the Key Laboratory of Developmental Genes and Human Disease, Southeast University, Nanjing 210096, China; Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu 226001, China; Corresponding authorSummary: In Drosophila, mutations in genes that prevent normal Ca2+ influx after light stimulation usually cause light-dependent retinal degeneration or neurodegeneration, detectable by defects in eye morphology. Here, we present a protocol based on electron microscopy (EM) to observe the morphological structure of photoreceptor cells in Drosophila. We detail how to fix, dehydrate, embed, and polymerize compound eye samples, followed by sectioning, post-staining, and image acquisition, to assess the eye morphology at the ultrastructural level.For complete details on the use and execution of this protocol, please refer to Gu et al. (2020). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166722003768Cell BiologyDevelopmental biologyMicroscopyModel OrganismsNeuroscience |
| spellingShingle | Qiuxiang Gu Yao Tian Junhai Han Protocol for electron microscopy of Drosophila photoreceptor cells STAR Protocols Cell Biology Developmental biology Microscopy Model Organisms Neuroscience |
| title | Protocol for electron microscopy of Drosophila photoreceptor cells |
| title_full | Protocol for electron microscopy of Drosophila photoreceptor cells |
| title_fullStr | Protocol for electron microscopy of Drosophila photoreceptor cells |
| title_full_unstemmed | Protocol for electron microscopy of Drosophila photoreceptor cells |
| title_short | Protocol for electron microscopy of Drosophila photoreceptor cells |
| title_sort | protocol for electron microscopy of drosophila photoreceptor cells |
| topic | Cell Biology Developmental biology Microscopy Model Organisms Neuroscience |
| url | http://www.sciencedirect.com/science/article/pii/S2666166722003768 |
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