A practical method to investigate the effect of volatile organic compounds emitted by rhizobacteria on plant growth under conditions of salt stress
Salinity is one of the causes that limit crop production. Plant Growth Promoting Rhizobacteria (PGPR) are beneficial soil bacteria that play a significant role in promoting plant growth. These microorganisms can produce their effect through the emission of Volatile Organic Compounds (VOCs). Most of...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-01-01
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| Series: | MethodsX |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215016123001012 |
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| author | Lorena del Rosario Cappellari Samanta Soledad Gil Tamara Belen Palermo Jimena Sofia Palermo Romina Meneguzzi Walter Giordano Erika Banchio |
| author_facet | Lorena del Rosario Cappellari Samanta Soledad Gil Tamara Belen Palermo Jimena Sofia Palermo Romina Meneguzzi Walter Giordano Erika Banchio |
| author_sort | Lorena del Rosario Cappellari |
| collection | DOAJ |
| description | Salinity is one of the causes that limit crop production. Plant Growth Promoting Rhizobacteria (PGPR) are beneficial soil bacteria that play a significant role in promoting plant growth. These microorganisms can produce their effect through the emission of Volatile Organic Compounds (VOCs). Most of the research to study the effects of microbial VOCs on plant growth has been carried out under controlled conditions using partitioned Petri dishes. In this article, we describe an alternative method that has the advantage of allowing long-term trials, being able to let the plant have a greater development in growth and height, without space limitation. In the proposed method, M. piperita were planted in glass jars containing Murashige and Skoog solid media, with a small glass vial containing Hoagland media inserted into the jar. This small vial was inoculated with the specified bacterium and served as the source of bacterial volatiles. This way plants were exposed to mVOCs without having any physical contact with the rhizobacteria. • The procedure allows studying the effect of microbial VOCs on plant growth. • It also allows longer trials, being able to let the plant develop more without space limitation. |
| first_indexed | 2024-03-13T03:33:15Z |
| format | Article |
| id | doaj.art-4674291d184944b28f1aa526005feadf |
| institution | Directory Open Access Journal |
| issn | 2215-0161 |
| language | English |
| last_indexed | 2024-03-13T03:33:15Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | MethodsX |
| spelling | doaj.art-4674291d184944b28f1aa526005feadf2023-06-24T05:17:20ZengElsevierMethodsX2215-01612023-01-0110102099A practical method to investigate the effect of volatile organic compounds emitted by rhizobacteria on plant growth under conditions of salt stressLorena del Rosario Cappellari0Samanta Soledad Gil1Tamara Belen Palermo2Jimena Sofia Palermo3Romina Meneguzzi4Walter Giordano5Erika Banchio6INBIAS Instituto de Biotecnología Ambiental y Salud (CONICET- Universidad Nacional de Río Cuarto), Campus Universitario, Río Cuarto 5800, ArgentinaINBIAS Instituto de Biotecnología Ambiental y Salud (CONICET- Universidad Nacional de Río Cuarto), Campus Universitario, Río Cuarto 5800, ArgentinaINBIAS Instituto de Biotecnología Ambiental y Salud (CONICET- Universidad Nacional de Río Cuarto), Campus Universitario, Río Cuarto 5800, ArgentinaINBIAS Instituto de Biotecnología Ambiental y Salud (CONICET- Universidad Nacional de Río Cuarto), Campus Universitario, Río Cuarto 5800, ArgentinaINBIAS Instituto de Biotecnología Ambiental y Salud (CONICET- Universidad Nacional de Río Cuarto), Campus Universitario, Río Cuarto 5800, ArgentinaINBIAS Instituto de Biotecnología Ambiental y Salud (CONICET- Universidad Nacional de Río Cuarto), Campus Universitario, Río Cuarto 5800, ArgentinaCorresponding author.; INBIAS Instituto de Biotecnología Ambiental y Salud (CONICET- Universidad Nacional de Río Cuarto), Campus Universitario, Río Cuarto 5800, ArgentinaSalinity is one of the causes that limit crop production. Plant Growth Promoting Rhizobacteria (PGPR) are beneficial soil bacteria that play a significant role in promoting plant growth. These microorganisms can produce their effect through the emission of Volatile Organic Compounds (VOCs). Most of the research to study the effects of microbial VOCs on plant growth has been carried out under controlled conditions using partitioned Petri dishes. In this article, we describe an alternative method that has the advantage of allowing long-term trials, being able to let the plant have a greater development in growth and height, without space limitation. In the proposed method, M. piperita were planted in glass jars containing Murashige and Skoog solid media, with a small glass vial containing Hoagland media inserted into the jar. This small vial was inoculated with the specified bacterium and served as the source of bacterial volatiles. This way plants were exposed to mVOCs without having any physical contact with the rhizobacteria. • The procedure allows studying the effect of microbial VOCs on plant growth. • It also allows longer trials, being able to let the plant develop more without space limitation.http://www.sciencedirect.com/science/article/pii/S2215016123001012Modeling the effect of microbial VOCs on aromatic and medicinal plants grown under saline stress |
| spellingShingle | Lorena del Rosario Cappellari Samanta Soledad Gil Tamara Belen Palermo Jimena Sofia Palermo Romina Meneguzzi Walter Giordano Erika Banchio A practical method to investigate the effect of volatile organic compounds emitted by rhizobacteria on plant growth under conditions of salt stress MethodsX Modeling the effect of microbial VOCs on aromatic and medicinal plants grown under saline stress |
| title | A practical method to investigate the effect of volatile organic compounds emitted by rhizobacteria on plant growth under conditions of salt stress |
| title_full | A practical method to investigate the effect of volatile organic compounds emitted by rhizobacteria on plant growth under conditions of salt stress |
| title_fullStr | A practical method to investigate the effect of volatile organic compounds emitted by rhizobacteria on plant growth under conditions of salt stress |
| title_full_unstemmed | A practical method to investigate the effect of volatile organic compounds emitted by rhizobacteria on plant growth under conditions of salt stress |
| title_short | A practical method to investigate the effect of volatile organic compounds emitted by rhizobacteria on plant growth under conditions of salt stress |
| title_sort | practical method to investigate the effect of volatile organic compounds emitted by rhizobacteria on plant growth under conditions of salt stress |
| topic | Modeling the effect of microbial VOCs on aromatic and medicinal plants grown under saline stress |
| url | http://www.sciencedirect.com/science/article/pii/S2215016123001012 |
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