Cellular and molecular studies of the effects of a selective COX-2 inhibitor celecoxib in the cardiac cell line H9c2 and their correlation with death mechanisms
Cardiovascular disease is one of the leading causes of death worldwide, and evidence indicates a correlation between the inflammatory process and cardiac dysfunction. Selective inhibitors of cyclooxygenase-2 (COX-2) enzyme are not recommended for long-term use because of potentially severe side effe...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2014-01-01
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| Series: | Brazilian Journal of Medical and Biological Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000100050&lng=en&tlng=en |
| _version_ | 1818555316557578240 |
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| author | K.K. Sakane C.J. Monteiro W. Silva A.R. Silva P.M. Santos K.F. Lima K.C.M. Moraes |
| author_facet | K.K. Sakane C.J. Monteiro W. Silva A.R. Silva P.M. Santos K.F. Lima K.C.M. Moraes |
| author_sort | K.K. Sakane |
| collection | DOAJ |
| description | Cardiovascular disease is one of the leading causes of death worldwide, and evidence indicates a correlation between the inflammatory process and cardiac dysfunction. Selective inhibitors of cyclooxygenase-2 (COX-2) enzyme are not recommended for long-term use because of potentially severe side effects to the heart. Considering this and the frequent prescribing of commercial celecoxib, the present study analyzed cellular and molecular effects of 1 and 10 µM celecoxib in a cell culture model. After a 24-h incubation, celecoxib reduced cell viability in a dose-dependent manner as also demonstrated in MTT assays. Furthermore, reverse transcription-polymerase chain reaction analysis showed that the drug modulated the expression level of genes related to death pathways, and Western blot analyses demonstrated a modulatory effect of the drug on COX-2 protein levels in cardiac cells. In addition, the results demonstrated a downregulation of prostaglandin E2 production by the cardiac cells incubated with celecoxib, in a dose-specific manner. These results are consistent with the decrease in cell viability and the presence of necrotic processes shown by Fourier transform infrared analysis, suggesting a direct correlation of prostanoids in cellular homeostasis and survival. |
| first_indexed | 2024-12-12T09:51:46Z |
| format | Article |
| id | doaj.art-4675afc8706e45f6b5d24926b94dec14 |
| institution | Directory Open Access Journal |
| issn | 1414-431X |
| language | English |
| last_indexed | 2024-12-12T09:51:46Z |
| publishDate | 2014-01-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-4675afc8706e45f6b5d24926b94dec142022-12-22T00:28:15ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research1414-431X2014-01-01471505910.1590/1414-431X20133028S0100-879X2014000100050Cellular and molecular studies of the effects of a selective COX-2 inhibitor celecoxib in the cardiac cell line H9c2 and their correlation with death mechanismsK.K. SakaneC.J. MonteiroW. SilvaA.R. SilvaP.M. SantosK.F. LimaK.C.M. MoraesCardiovascular disease is one of the leading causes of death worldwide, and evidence indicates a correlation between the inflammatory process and cardiac dysfunction. Selective inhibitors of cyclooxygenase-2 (COX-2) enzyme are not recommended for long-term use because of potentially severe side effects to the heart. Considering this and the frequent prescribing of commercial celecoxib, the present study analyzed cellular and molecular effects of 1 and 10 µM celecoxib in a cell culture model. After a 24-h incubation, celecoxib reduced cell viability in a dose-dependent manner as also demonstrated in MTT assays. Furthermore, reverse transcription-polymerase chain reaction analysis showed that the drug modulated the expression level of genes related to death pathways, and Western blot analyses demonstrated a modulatory effect of the drug on COX-2 protein levels in cardiac cells. In addition, the results demonstrated a downregulation of prostaglandin E2 production by the cardiac cells incubated with celecoxib, in a dose-specific manner. These results are consistent with the decrease in cell viability and the presence of necrotic processes shown by Fourier transform infrared analysis, suggesting a direct correlation of prostanoids in cellular homeostasis and survival.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000100050&lng=en&tlng=enCellular and molecular analysesCell deathCyclooxygenase-2 inhibitorH9c2 cardiac cell line |
| spellingShingle | K.K. Sakane C.J. Monteiro W. Silva A.R. Silva P.M. Santos K.F. Lima K.C.M. Moraes Cellular and molecular studies of the effects of a selective COX-2 inhibitor celecoxib in the cardiac cell line H9c2 and their correlation with death mechanisms Brazilian Journal of Medical and Biological Research Cellular and molecular analyses Cell death Cyclooxygenase-2 inhibitor H9c2 cardiac cell line |
| title | Cellular and molecular studies of the effects of a selective COX-2 inhibitor celecoxib in the cardiac cell line H9c2 and their correlation with death mechanisms |
| title_full | Cellular and molecular studies of the effects of a selective COX-2 inhibitor celecoxib in the cardiac cell line H9c2 and their correlation with death mechanisms |
| title_fullStr | Cellular and molecular studies of the effects of a selective COX-2 inhibitor celecoxib in the cardiac cell line H9c2 and their correlation with death mechanisms |
| title_full_unstemmed | Cellular and molecular studies of the effects of a selective COX-2 inhibitor celecoxib in the cardiac cell line H9c2 and their correlation with death mechanisms |
| title_short | Cellular and molecular studies of the effects of a selective COX-2 inhibitor celecoxib in the cardiac cell line H9c2 and their correlation with death mechanisms |
| title_sort | cellular and molecular studies of the effects of a selective cox 2 inhibitor celecoxib in the cardiac cell line h9c2 and their correlation with death mechanisms |
| topic | Cellular and molecular analyses Cell death Cyclooxygenase-2 inhibitor H9c2 cardiac cell line |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000100050&lng=en&tlng=en |
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