Engineering a Ca++-Sensitive (Bio)Sensor from the Pore-Module of a Potassium Channel
Signals recorded at the cell membrane are meaningful indicators of the physiological vs. pathological state of a cell and will become useful diagnostic elements in nanomedicine. In this project we present a coherent strategy for the design and fabrication of a bio-nano-sensor that monitors changes...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2015-02-01
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| Series: | Sensors |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1424-8220/15/3/4913 |
| _version_ | 1811184237418643456 |
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| author | Mattia Lorenzo DiFrancesco Sabrina Gazzarrini Cristina Arrigoni Giulia Romani Gerhard Thiel Anna Moroni |
| author_facet | Mattia Lorenzo DiFrancesco Sabrina Gazzarrini Cristina Arrigoni Giulia Romani Gerhard Thiel Anna Moroni |
| author_sort | Mattia Lorenzo DiFrancesco |
| collection | DOAJ |
| description | Signals recorded at the cell membrane are meaningful indicators of the physiological vs. pathological state of a cell and will become useful diagnostic elements in nanomedicine. In this project we present a coherent strategy for the design and fabrication of a bio-nano-sensor that monitors changes in intracellular cell calcium concentration and allows an easy read out by converting the calcium signal into an electrical current in the range of microampere that can be easily measured by conventional cell electrophysiology apparatus. |
| first_indexed | 2024-04-11T13:10:19Z |
| format | Article |
| id | doaj.art-467b8664f2f74d91845868627c3aa8ae |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T13:10:19Z |
| publishDate | 2015-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-467b8664f2f74d91845868627c3aa8ae2022-12-22T04:22:37ZengMDPI AGSensors1424-82202015-02-011534913492410.3390/s150304913s150304913Engineering a Ca++-Sensitive (Bio)Sensor from the Pore-Module of a Potassium ChannelMattia Lorenzo DiFrancesco0Sabrina Gazzarrini1Cristina Arrigoni2Giulia Romani3Gerhard Thiel4Anna Moroni5Department of Biosciences, University of Milan, Via Celoria 26, Milano 20133, ItalyDepartment of Biosciences, University of Milan, Via Celoria 26, Milano 20133, ItalyDepartment of Biosciences, University of Milan, Via Celoria 26, Milano 20133, ItalyInstitute of Biophysics-Milan section, National Research Council, Via Celoria 26, Milano 20133, ItalyDepartment of Biology, Technische Universität Darmstadt, Schnittspahnstrasse 3, Darmstadt 64287, GermanyDepartment of Biosciences, University of Milan, Via Celoria 26, Milano 20133, ItalySignals recorded at the cell membrane are meaningful indicators of the physiological vs. pathological state of a cell and will become useful diagnostic elements in nanomedicine. In this project we present a coherent strategy for the design and fabrication of a bio-nano-sensor that monitors changes in intracellular cell calcium concentration and allows an easy read out by converting the calcium signal into an electrical current in the range of microampere that can be easily measured by conventional cell electrophysiology apparatus.http://www.mdpi.com/1424-8220/15/3/4913biosensorKcvcalciumcalmodulinsynthetic ion channel |
| spellingShingle | Mattia Lorenzo DiFrancesco Sabrina Gazzarrini Cristina Arrigoni Giulia Romani Gerhard Thiel Anna Moroni Engineering a Ca++-Sensitive (Bio)Sensor from the Pore-Module of a Potassium Channel Sensors biosensor Kcv calcium calmodulin synthetic ion channel |
| title | Engineering a Ca++-Sensitive (Bio)Sensor from the Pore-Module of a Potassium Channel |
| title_full | Engineering a Ca++-Sensitive (Bio)Sensor from the Pore-Module of a Potassium Channel |
| title_fullStr | Engineering a Ca++-Sensitive (Bio)Sensor from the Pore-Module of a Potassium Channel |
| title_full_unstemmed | Engineering a Ca++-Sensitive (Bio)Sensor from the Pore-Module of a Potassium Channel |
| title_short | Engineering a Ca++-Sensitive (Bio)Sensor from the Pore-Module of a Potassium Channel |
| title_sort | engineering a ca sensitive bio sensor from the pore module of a potassium channel |
| topic | biosensor Kcv calcium calmodulin synthetic ion channel |
| url | http://www.mdpi.com/1424-8220/15/3/4913 |
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