Spectrofluorimetry method for determining ascorbic acid(荧光光度法测定维生素C)
维生素C与2，3-二氨基萘（DAN）在pH=10.2～10.5时能发生缩合反应，生成强荧光的杂氮环缩合物，据此建立了一种简便、灵敏测定抗坏血酸的新方法.在激发波长400 nm，发射波长520 nm处测定其荧光强度.维生素C浓度在2~300 µg • mL-1范围内与其荧光强度呈良好的线性关系，相关系数为0.9993，其检出限为0.6 µg • mL-1(S/N = 3).利用本法进行回收试验与样品测定都取得了令人满意的结果....
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| Format: | Article |
| Language: | zho |
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Zhejiang University Press
2001-09-01
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| Series: | Zhejiang Daxue xuebao. Lixue ban |
| Subjects: | |
| Online Access: | https://doi.org/zjup/1008-9497.2001.28.5.542-546 |
| _version_ | 1797236466541133824 |
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| author | TONGChang-lun(童裳伦) |
| author_facet | TONGChang-lun(童裳伦) |
| author_sort | TONGChang-lun(童裳伦) |
| collection | DOAJ |
| description | 维生素C与2，3-二氨基萘（DAN）在pH=10.2～10.5时能发生缩合反应，生成强荧光的杂氮环缩合物，据此建立了一种简便、灵敏测定抗坏血酸的新方法.在激发波长400 nm，发射波长520 nm处测定其荧光强度.维生素C浓度在2~300 µg • mL-1范围内与其荧光强度呈良好的线性关系，相关系数为0.9993，其检出限为0.6 µg • mL-1(S/N = 3).利用本法进行回收试验与样品测定都取得了令人满意的结果. |
| first_indexed | 2024-04-24T17:04:18Z |
| format | Article |
| id | doaj.art-467bbb6e31a74d6eb5b10e3125d3c09f |
| institution | Directory Open Access Journal |
| issn | 1008-9497 |
| language | zho |
| last_indexed | 2024-04-24T17:04:18Z |
| publishDate | 2001-09-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | Zhejiang Daxue xuebao. Lixue ban |
| spelling | doaj.art-467bbb6e31a74d6eb5b10e3125d3c09f2024-03-29T01:58:17ZzhoZhejiang University PressZhejiang Daxue xuebao. Lixue ban1008-94972001-09-01285542546zjup/1008-9497.2001.28.5.542-546Spectrofluorimetry method for determining ascorbic acid(荧光光度法测定维生素C)TONGChang-lun(童裳伦)0Institute of Environmental Science, Zhejiang University, Hangzhou 310027, China(浙江大学环境科学研究所，浙江 杭州 310027)维生素C与2，3-二氨基萘（DAN）在pH=10.2～10.5时能发生缩合反应，生成强荧光的杂氮环缩合物，据此建立了一种简便、灵敏测定抗坏血酸的新方法.在激发波长400 nm，发射波长520 nm处测定其荧光强度.维生素C浓度在2~300 µg • mL-1范围内与其荧光强度呈良好的线性关系，相关系数为0.9993，其检出限为0.6 µg • mL-1(S/N = 3).利用本法进行回收试验与样品测定都取得了令人满意的结果.https://doi.org/zjup/1008-9497.2001.28.5.542-546荧光光度法维生素c |
| spellingShingle | TONGChang-lun(童裳伦) Spectrofluorimetry method for determining ascorbic acid(荧光光度法测定维生素C) Zhejiang Daxue xuebao. Lixue ban 荧光光度法 维生素c |
| title | Spectrofluorimetry method for determining ascorbic acid(荧光光度法测定维生素C) |
| title_full | Spectrofluorimetry method for determining ascorbic acid(荧光光度法测定维生素C) |
| title_fullStr | Spectrofluorimetry method for determining ascorbic acid(荧光光度法测定维生素C) |
| title_full_unstemmed | Spectrofluorimetry method for determining ascorbic acid(荧光光度法测定维生素C) |
| title_short | Spectrofluorimetry method for determining ascorbic acid(荧光光度法测定维生素C) |
| title_sort | spectrofluorimetry method for determining ascorbic acid 荧光光度法测定维生素c |
| topic | 荧光光度法 维生素c |
| url | https://doi.org/zjup/1008-9497.2001.28.5.542-546 |
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