Extended Dual-Focus Microscopy for Ratiometric-Based 3D Movement Tracking
Imaging the three-dimensional movement of small organelles in living cells can provide key information for the dynamics of drug delivery and virus transmission in biomedical disciplines. To stably monitor such intracellular motion using microscope, long depth of field along optical axis and accurate...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-09-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/10/18/6243 |
| _version_ | 1797554262321922048 |
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| author | Seohyun Lee Hyuno Kim Hideo Higuchi |
| author_facet | Seohyun Lee Hyuno Kim Hideo Higuchi |
| author_sort | Seohyun Lee |
| collection | DOAJ |
| description | Imaging the three-dimensional movement of small organelles in living cells can provide key information for the dynamics of drug delivery and virus transmission in biomedical disciplines. To stably monitor such intracellular motion using microscope, long depth of field along optical axis and accurate three-dimensional tracking are simultaneously required. In the present work, we suggest an extended dual-focus optics microscopy system by combining a bifocal plane imaging scheme and objective lens oscillation, which enables accurate localization for a long axial range. The proposed system exploits high-resolution functionality by concatenating partial calibration result acquired each axial imaging level, maintaining the practical advantages of ratiometric method. |
| first_indexed | 2024-03-10T16:28:31Z |
| format | Article |
| id | doaj.art-4683a1c484f045fd98ec64ae3a94f363 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T16:28:31Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-4683a1c484f045fd98ec64ae3a94f3632023-11-20T12:59:55ZengMDPI AGApplied Sciences2076-34172020-09-011018624310.3390/app10186243Extended Dual-Focus Microscopy for Ratiometric-Based 3D Movement TrackingSeohyun Lee0Hyuno Kim1Hideo Higuchi2Data Science Research Division, Information Technology Center, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-8656, JapanData Science Research Division, Information Technology Center, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-8656, JapanDepartment of Physics, Graduate School of Science, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-0033, JapanImaging the three-dimensional movement of small organelles in living cells can provide key information for the dynamics of drug delivery and virus transmission in biomedical disciplines. To stably monitor such intracellular motion using microscope, long depth of field along optical axis and accurate three-dimensional tracking are simultaneously required. In the present work, we suggest an extended dual-focus optics microscopy system by combining a bifocal plane imaging scheme and objective lens oscillation, which enables accurate localization for a long axial range. The proposed system exploits high-resolution functionality by concatenating partial calibration result acquired each axial imaging level, maintaining the practical advantages of ratiometric method.https://www.mdpi.com/2076-3417/10/18/6243vesicle transportdual-focus opticsnanoparticle trackingthree-dimensional microscopy |
| spellingShingle | Seohyun Lee Hyuno Kim Hideo Higuchi Extended Dual-Focus Microscopy for Ratiometric-Based 3D Movement Tracking Applied Sciences vesicle transport dual-focus optics nanoparticle tracking three-dimensional microscopy |
| title | Extended Dual-Focus Microscopy for Ratiometric-Based 3D Movement Tracking |
| title_full | Extended Dual-Focus Microscopy for Ratiometric-Based 3D Movement Tracking |
| title_fullStr | Extended Dual-Focus Microscopy for Ratiometric-Based 3D Movement Tracking |
| title_full_unstemmed | Extended Dual-Focus Microscopy for Ratiometric-Based 3D Movement Tracking |
| title_short | Extended Dual-Focus Microscopy for Ratiometric-Based 3D Movement Tracking |
| title_sort | extended dual focus microscopy for ratiometric based 3d movement tracking |
| topic | vesicle transport dual-focus optics nanoparticle tracking three-dimensional microscopy |
| url | https://www.mdpi.com/2076-3417/10/18/6243 |
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