Analysis of outer membrane vesicle associated proteins isolated from the plant pathogenic bacterium <it>Xanthomonas campestris </it>pv. campestris
<p>Abstract</p> <p>Background</p> <p>Outer membrane vesicles (OMVs) are released from the outer membrane of many Gram-negative bacteria. These extracellular compartments are known to transport compounds involved in cell-cell signalling as well as virulence associated pr...
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| Format: | Article |
| Language: | English |
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BMC
2008-06-01
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| Series: | BMC Microbiology |
| Online Access: | http://www.biomedcentral.com/1471-2180/8/87 |
| _version_ | 1818154917743820800 |
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| author | Niehaus Karsten Vorhölter Frank-Jörg Sidhu Vishaldeep K Watt Steven A |
| author_facet | Niehaus Karsten Vorhölter Frank-Jörg Sidhu Vishaldeep K Watt Steven A |
| author_sort | Niehaus Karsten |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Outer membrane vesicles (OMVs) are released from the outer membrane of many Gram-negative bacteria. These extracellular compartments are known to transport compounds involved in cell-cell signalling as well as virulence associated proteins, e.g. the cytolysine from enterotoxic <it>E. coli</it>.</p> <p>Results</p> <p>We have demonstrated that <it>Xanthomonas campestris </it>pv. campestris (<it>Xcc</it>) releases OMVs into the culture supernatant during growth. A proteome study identified 31 different proteins that associate with the OMV fraction of which half are virulence-associated. A comparison with the most abundant outer membrane (OM) proteins revealed that some proteins are enriched in the OMV fraction. This may be connected to differences in the LPS composition between the OMVs and the OM. Furthermore, a comparison of the OMV proteomes from two different culture media indicated that the culture conditions have an impact on the protein composition. Interestingly, the proteins that are common to both culture conditions are mainly involved in virulence.</p> <p>Conclusion</p> <p>Outer membrane vesicles released from the OM of <it>Xcc </it>contain membrane- and virulence-associated proteins. Future experiments will prove whether these structures can serve as "vehicles" for the transport of virulence factors into the host membrane.</p> |
| first_indexed | 2024-12-11T14:34:08Z |
| format | Article |
| id | doaj.art-46849b21ddf644908411a49d76ab32fd |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-11T14:34:08Z |
| publishDate | 2008-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-46849b21ddf644908411a49d76ab32fd2022-12-22T01:02:16ZengBMCBMC Microbiology1471-21802008-06-01818710.1186/1471-2180-8-87Analysis of outer membrane vesicle associated proteins isolated from the plant pathogenic bacterium <it>Xanthomonas campestris </it>pv. campestrisNiehaus KarstenVorhölter Frank-JörgSidhu Vishaldeep KWatt Steven A<p>Abstract</p> <p>Background</p> <p>Outer membrane vesicles (OMVs) are released from the outer membrane of many Gram-negative bacteria. These extracellular compartments are known to transport compounds involved in cell-cell signalling as well as virulence associated proteins, e.g. the cytolysine from enterotoxic <it>E. coli</it>.</p> <p>Results</p> <p>We have demonstrated that <it>Xanthomonas campestris </it>pv. campestris (<it>Xcc</it>) releases OMVs into the culture supernatant during growth. A proteome study identified 31 different proteins that associate with the OMV fraction of which half are virulence-associated. A comparison with the most abundant outer membrane (OM) proteins revealed that some proteins are enriched in the OMV fraction. This may be connected to differences in the LPS composition between the OMVs and the OM. Furthermore, a comparison of the OMV proteomes from two different culture media indicated that the culture conditions have an impact on the protein composition. Interestingly, the proteins that are common to both culture conditions are mainly involved in virulence.</p> <p>Conclusion</p> <p>Outer membrane vesicles released from the OM of <it>Xcc </it>contain membrane- and virulence-associated proteins. Future experiments will prove whether these structures can serve as "vehicles" for the transport of virulence factors into the host membrane.</p>http://www.biomedcentral.com/1471-2180/8/87 |
| spellingShingle | Niehaus Karsten Vorhölter Frank-Jörg Sidhu Vishaldeep K Watt Steven A Analysis of outer membrane vesicle associated proteins isolated from the plant pathogenic bacterium <it>Xanthomonas campestris </it>pv. campestris BMC Microbiology |
| title | Analysis of outer membrane vesicle associated proteins isolated from the plant pathogenic bacterium <it>Xanthomonas campestris </it>pv. campestris |
| title_full | Analysis of outer membrane vesicle associated proteins isolated from the plant pathogenic bacterium <it>Xanthomonas campestris </it>pv. campestris |
| title_fullStr | Analysis of outer membrane vesicle associated proteins isolated from the plant pathogenic bacterium <it>Xanthomonas campestris </it>pv. campestris |
| title_full_unstemmed | Analysis of outer membrane vesicle associated proteins isolated from the plant pathogenic bacterium <it>Xanthomonas campestris </it>pv. campestris |
| title_short | Analysis of outer membrane vesicle associated proteins isolated from the plant pathogenic bacterium <it>Xanthomonas campestris </it>pv. campestris |
| title_sort | analysis of outer membrane vesicle associated proteins isolated from the plant pathogenic bacterium it xanthomonas campestris it pv campestris |
| url | http://www.biomedcentral.com/1471-2180/8/87 |
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