Lipofection-Mediated Introduction of CRISPR/Cas9 System into Porcine Oocytes and Embryos

Lipofection-Mediated Introduction of CRISPR/Cas9 System into Porcine Oocytes and Embryos

Liposome-mediated gene transfer has become an alternative method for establishing a gene targeting framework, and the production of mutant animals may be feasible even in laboratories without specialized equipment. However, how this system functions in mammalian oocytes and embryos remains unclear....

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Bibliographic Details
Main Authors: Maki Hirata, Manita Wittayarat, Zhao Namula, Quynh Anh Le, Qingyi Lin, Koki Takebayashi, Chommanart Thongkittidilok, Fuminori Tanihara, Takeshige Otoi
Format: Article
Language:English
Published: MDPI AG 2021-02-01
Series:Animals
Subjects:
CRISPR/Cas9
embryo
lipofection
oocyte
pig
Online Access:https://www.mdpi.com/2076-2615/11/2/578

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