Ultrastructural examination of cryodamage in Paracentrotus lividus eggs during cryopreservation
Abstract This study examinates the challenges of cryopreserving sea urchin (Paracentrotus lividus) eggs, a task hindered by factors like low membrane permeability and high sensitivity to cryoprotective agents (CPAs). While successful cryopreservation has been achieved for some marine invertebrates,...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-04-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-57905-2 |
| _version_ | 1797199393970978816 |
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| author | S. Campos J. Troncoso E. Paredes |
| author_facet | S. Campos J. Troncoso E. Paredes |
| author_sort | S. Campos |
| collection | DOAJ |
| description | Abstract This study examinates the challenges of cryopreserving sea urchin (Paracentrotus lividus) eggs, a task hindered by factors like low membrane permeability and high sensitivity to cryoprotective agents (CPAs). While successful cryopreservation has been achieved for some marine invertebrates, eggs remain problematic due to their unique characteristics. The study explores the impact of various CPAs and cryopreservation techniques on sea urchin eggs, employing scanning and transmission electron microscopy to analyze cellular damage. The findings reveal that exposure to low CPA concentrations (0.5 M) did not induce significant damage to eggs. However, high concentrations (3 M) proved highly detrimental. Every cryopreservation approach investigated in this study resulted in irreversible damage to the sea urchin eggs, rendering them nonviable for future use. The research sheds light on the importance of understanding the structural alterations induced by CPAs and cryopreservation methods. This knowledge is essential for refining cryopreservation methods, potentially paving the way for successful preservation of these challenging cells. |
| first_indexed | 2024-04-24T07:15:03Z |
| format | Article |
| id | doaj.art-468ada13599c49bcb6a9cfdb718e2127 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-24T07:15:03Z |
| publishDate | 2024-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-468ada13599c49bcb6a9cfdb718e21272024-04-21T11:19:44ZengNature PortfolioScientific Reports2045-23222024-04-0114111010.1038/s41598-024-57905-2Ultrastructural examination of cryodamage in Paracentrotus lividus eggs during cryopreservationS. Campos0J. Troncoso1E. Paredes2Centro de Investigación Mariña (CIM), Departamento de Ecoloxía e Bioloxía Animal, Grupo ECOCOST, Universidade de VigoCentro de Investigación Mariña (CIM), Departamento de Ecoloxía e Bioloxía Animal, Grupo ECOCOST, Universidade de VigoCentro de Investigación Mariña (CIM), Departamento de Ecoloxía e Bioloxía Animal, Grupo ECOCOST, Universidade de VigoAbstract This study examinates the challenges of cryopreserving sea urchin (Paracentrotus lividus) eggs, a task hindered by factors like low membrane permeability and high sensitivity to cryoprotective agents (CPAs). While successful cryopreservation has been achieved for some marine invertebrates, eggs remain problematic due to their unique characteristics. The study explores the impact of various CPAs and cryopreservation techniques on sea urchin eggs, employing scanning and transmission electron microscopy to analyze cellular damage. The findings reveal that exposure to low CPA concentrations (0.5 M) did not induce significant damage to eggs. However, high concentrations (3 M) proved highly detrimental. Every cryopreservation approach investigated in this study resulted in irreversible damage to the sea urchin eggs, rendering them nonviable for future use. The research sheds light on the importance of understanding the structural alterations induced by CPAs and cryopreservation methods. This knowledge is essential for refining cryopreservation methods, potentially paving the way for successful preservation of these challenging cells.https://doi.org/10.1038/s41598-024-57905-2 |
| spellingShingle | S. Campos J. Troncoso E. Paredes Ultrastructural examination of cryodamage in Paracentrotus lividus eggs during cryopreservation Scientific Reports |
| title | Ultrastructural examination of cryodamage in Paracentrotus lividus eggs during cryopreservation |
| title_full | Ultrastructural examination of cryodamage in Paracentrotus lividus eggs during cryopreservation |
| title_fullStr | Ultrastructural examination of cryodamage in Paracentrotus lividus eggs during cryopreservation |
| title_full_unstemmed | Ultrastructural examination of cryodamage in Paracentrotus lividus eggs during cryopreservation |
| title_short | Ultrastructural examination of cryodamage in Paracentrotus lividus eggs during cryopreservation |
| title_sort | ultrastructural examination of cryodamage in paracentrotus lividus eggs during cryopreservation |
| url | https://doi.org/10.1038/s41598-024-57905-2 |
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