Phytochemical Properties, Antioxidant and in Vitro/in Silico Anti-Acetylcholinesterase Activities of Hypericum heterophyllum Leaf from Türkiye
Abstract Methanol, ethanol, acetone and chloroform extracts of powdered Hypericum heterophyllum prepared from leafs were investigated for their (i) phytochemical constituents, which was evaluated by LC-ESI-MS/MS and various known total bioactive methods; (ii) antioxidant capacity, which was evaluate...
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| Language: | English |
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Instituto de Tecnologia do Paraná (Tecpar)
2024-01-01
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| Series: | Brazilian Archives of Biology and Technology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132024000100402&lng=en&tlng=en |
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| author | Cennet Yaman Ramazan Erenler Mehmet Nuri Atalar Şevki Adem Ufuk Koca Çalişkan |
| author_facet | Cennet Yaman Ramazan Erenler Mehmet Nuri Atalar Şevki Adem Ufuk Koca Çalişkan |
| author_sort | Cennet Yaman |
| collection | DOAJ |
| description | Abstract Methanol, ethanol, acetone and chloroform extracts of powdered Hypericum heterophyllum prepared from leafs were investigated for their (i) phytochemical constituents, which was evaluated by LC-ESI-MS/MS and various known total bioactive methods; (ii) antioxidant capacity, which was evaluated by several in vitro assays such as DPPH, ABTS, H2O2, superoxide radical, and metal chelating; (iii) acetylcholinesterase (AChE) inhibitory activity; (iv) active constituents of the extracts with AChE in silico docking. Phytochemical analysis revealed that none of the extracts contained hypericin, pseudohypericin and hyperforin. Among the identified compounds, ethanol and acetone extracts had the highest number of compounds. The highest value of total phenolic content (132.7 mg GAE/g) was obtained with methanol extract, whereas the highest values of total flavonoid (61.8 mg QE/g) and flavanol (0.89 mg CE/g) content were recorded in acetone extract. Methanol extract displayed the most DPPH activity (24.9 mg TE/g), but ethanol extract (59.8 mg TE/g) showed the highest ABTS activity. Acetone extract displayed the best hydroxyl and superoxide radical scavenging activities with the values of 351.5 mg AAE/g, and 197.3 mg TE/g respectively. The chloroform extract exhibited the best AChE inhibition activity (11.07 µg/ml). In the docking study, quercetin-7-O-glucoside, diosgenin, and quercetin-3-glucoside were calculated to have a stronger MolDock Score than the reference ligand Donepezil. Our docking results have indicated that extracts contain compounds with a high affinity against the acetylcholinesterase enzyme. These data suggest that these extracts can be potentially important antioxidant supplements and drug candidates for AChE inhibition activity. |
| first_indexed | 2024-03-08T12:06:32Z |
| format | Article |
| id | doaj.art-468bf80d01724c8387f5ead9226aa43e |
| institution | Directory Open Access Journal |
| issn | 1678-4324 |
| language | English |
| last_indexed | 2024-03-08T12:06:32Z |
| publishDate | 2024-01-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-468bf80d01724c8387f5ead9226aa43e2024-01-23T07:38:01ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1678-43242024-01-016710.1590/1678-4324-2024230043Phytochemical Properties, Antioxidant and in Vitro/in Silico Anti-Acetylcholinesterase Activities of Hypericum heterophyllum Leaf from TürkiyeCennet Yamanhttps://orcid.org/0000-0002-2364-8171Ramazan Erenlerhttps://orcid.org/0000-0002-0505-3190Mehmet Nuri Atalarhttps://orcid.org/0000-0003-2993-2605Şevki Ademhttps://orcid.org/0000-0003-2146-5870Ufuk Koca Çalişkanhttps://orcid.org/0000-0002-5216-7588Abstract Methanol, ethanol, acetone and chloroform extracts of powdered Hypericum heterophyllum prepared from leafs were investigated for their (i) phytochemical constituents, which was evaluated by LC-ESI-MS/MS and various known total bioactive methods; (ii) antioxidant capacity, which was evaluated by several in vitro assays such as DPPH, ABTS, H2O2, superoxide radical, and metal chelating; (iii) acetylcholinesterase (AChE) inhibitory activity; (iv) active constituents of the extracts with AChE in silico docking. Phytochemical analysis revealed that none of the extracts contained hypericin, pseudohypericin and hyperforin. Among the identified compounds, ethanol and acetone extracts had the highest number of compounds. The highest value of total phenolic content (132.7 mg GAE/g) was obtained with methanol extract, whereas the highest values of total flavonoid (61.8 mg QE/g) and flavanol (0.89 mg CE/g) content were recorded in acetone extract. Methanol extract displayed the most DPPH activity (24.9 mg TE/g), but ethanol extract (59.8 mg TE/g) showed the highest ABTS activity. Acetone extract displayed the best hydroxyl and superoxide radical scavenging activities with the values of 351.5 mg AAE/g, and 197.3 mg TE/g respectively. The chloroform extract exhibited the best AChE inhibition activity (11.07 µg/ml). In the docking study, quercetin-7-O-glucoside, diosgenin, and quercetin-3-glucoside were calculated to have a stronger MolDock Score than the reference ligand Donepezil. Our docking results have indicated that extracts contain compounds with a high affinity against the acetylcholinesterase enzyme. These data suggest that these extracts can be potentially important antioxidant supplements and drug candidates for AChE inhibition activity.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132024000100402&lng=en&tlng=enHypericum heterophyllumphytochemicalbiological activityin silico dockingsolvent |
| spellingShingle | Cennet Yaman Ramazan Erenler Mehmet Nuri Atalar Şevki Adem Ufuk Koca Çalişkan Phytochemical Properties, Antioxidant and in Vitro/in Silico Anti-Acetylcholinesterase Activities of Hypericum heterophyllum Leaf from Türkiye Brazilian Archives of Biology and Technology Hypericum heterophyllum phytochemical biological activity in silico docking solvent |
| title | Phytochemical Properties, Antioxidant and in Vitro/in Silico Anti-Acetylcholinesterase Activities of Hypericum heterophyllum Leaf from Türkiye |
| title_full | Phytochemical Properties, Antioxidant and in Vitro/in Silico Anti-Acetylcholinesterase Activities of Hypericum heterophyllum Leaf from Türkiye |
| title_fullStr | Phytochemical Properties, Antioxidant and in Vitro/in Silico Anti-Acetylcholinesterase Activities of Hypericum heterophyllum Leaf from Türkiye |
| title_full_unstemmed | Phytochemical Properties, Antioxidant and in Vitro/in Silico Anti-Acetylcholinesterase Activities of Hypericum heterophyllum Leaf from Türkiye |
| title_short | Phytochemical Properties, Antioxidant and in Vitro/in Silico Anti-Acetylcholinesterase Activities of Hypericum heterophyllum Leaf from Türkiye |
| title_sort | phytochemical properties antioxidant and in vitro in silico anti acetylcholinesterase activities of hypericum heterophyllum leaf from turkiye |
| topic | Hypericum heterophyllum phytochemical biological activity in silico docking solvent |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132024000100402&lng=en&tlng=en |
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