Transcriptome and Gene Regulatory Network Analyses Reveal New Transcription Factors in Mature Fruit Associated with Harvest Date in <i>Prunus persica</i>
Harvest date is a critical parameter for producers and consumers regarding agro-industrial performance. It involves a pleiotropic effect controlling the development of other fruit quality traits through finely controlling regulatory mechanisms. Fruit ripening is a process in which various signals an...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/24/3473 |
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| author | Gerardo Núñez-Lillo Wellasmin Pérez-Reyes Anibal Riveros Victoria Lillo-Carmona Karin Rothkegel José Miguel Álvarez Francisca Blanco-Herrera Romina Pedreschi Reinaldo Campos-Vargas Claudio Meneses |
| author_facet | Gerardo Núñez-Lillo Wellasmin Pérez-Reyes Anibal Riveros Victoria Lillo-Carmona Karin Rothkegel José Miguel Álvarez Francisca Blanco-Herrera Romina Pedreschi Reinaldo Campos-Vargas Claudio Meneses |
| author_sort | Gerardo Núñez-Lillo |
| collection | DOAJ |
| description | Harvest date is a critical parameter for producers and consumers regarding agro-industrial performance. It involves a pleiotropic effect controlling the development of other fruit quality traits through finely controlling regulatory mechanisms. Fruit ripening is a process in which various signals and biological events co-occur and are regulated by hormone signaling that produces the accumulation/degradation of multiple compounds. However, the regulatory mechanisms that control the hormone signaling involved in fruit development and ripening are still unclear. To investigate the issue, we used individuals with early, middle and late harvest dates from a peach segregating population to identify regulatory candidate genes controlling fruit quality traits at the harvest stage and validate them in contrasting peach varieties for this trait. We identified 467 and 654 differentially expressed genes for early and late harvest through a transcriptomic approach. In addition, using the Arabidopsis DAP-seq database and network analysis, six transcription factors were selected. Our results suggest significant hormonal balance and cell wall composition/structure differences between early and late harvest samples. Thus, we propose that higher expression levels of the transcription factors <i>HB7</i>, <i>ERF017</i> and <i>WRKY70</i> in early harvest individuals would induce the expression of genes associated with the jasmonic acid pathway, photosynthesis and gibberellins inhibition. While on the other hand, the high expression levels of <i>LHY</i>, <i>CDF3</i> and <i>NAC083</i> in late harvest individuals would promote the induction of genes associated with abscisic acid biosynthesis, auxins and cell wall remodeling. |
| first_indexed | 2024-03-09T15:56:20Z |
| format | Article |
| id | doaj.art-468ca7b6041641e5b8ac3e18c5f9d6ab |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T15:56:20Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-468ca7b6041641e5b8ac3e18c5f9d6ab2023-11-24T17:27:53ZengMDPI AGPlants2223-77472022-12-011124347310.3390/plants11243473Transcriptome and Gene Regulatory Network Analyses Reveal New Transcription Factors in Mature Fruit Associated with Harvest Date in <i>Prunus persica</i>Gerardo Núñez-Lillo0Wellasmin Pérez-Reyes1Anibal Riveros2Victoria Lillo-Carmona3Karin Rothkegel4José Miguel Álvarez5Francisca Blanco-Herrera6Romina Pedreschi7Reinaldo Campos-Vargas8Claudio Meneses9Escuela de Agronomía, Facultad de Ciencias Agronómicas y de los Alimentos, Pontificia Universidad Católica de Valparaíso, Quillota 2260000, ChileCentro de Biotecnología Vegetal, Facultad de Ciencias de la Vida, Universidad Andrés Bello, Santiago 8370186, ChileDepartamento de Fruticultura y Enología, Facultad de Agronomía e Ingeniería Forestal, Pontificia Universidad Católica de Chile, Santiago 7820436, ChileDepartamento de Fruticultura y Enología, Facultad de Agronomía e Ingeniería Forestal, Pontificia Universidad Católica de Chile, Santiago 7820436, ChileDepartamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago 8331150, ChileCentro de Biotecnología Vegetal, Facultad de Ciencias de la Vida, Universidad Andrés Bello, Santiago 8370186, ChileCentro de Biotecnología Vegetal, Facultad de Ciencias de la Vida, Universidad Andrés Bello, Santiago 8370186, ChileEscuela de Agronomía, Facultad de Ciencias Agronómicas y de los Alimentos, Pontificia Universidad Católica de Valparaíso, Quillota 2260000, ChileDepartamento de Producción Agrícola, Facultad de Ciencias Agronómicas, Universidad de Chile, Santiago 8820808, ChileDepartamento de Fruticultura y Enología, Facultad de Agronomía e Ingeniería Forestal, Pontificia Universidad Católica de Chile, Santiago 7820436, ChileHarvest date is a critical parameter for producers and consumers regarding agro-industrial performance. It involves a pleiotropic effect controlling the development of other fruit quality traits through finely controlling regulatory mechanisms. Fruit ripening is a process in which various signals and biological events co-occur and are regulated by hormone signaling that produces the accumulation/degradation of multiple compounds. However, the regulatory mechanisms that control the hormone signaling involved in fruit development and ripening are still unclear. To investigate the issue, we used individuals with early, middle and late harvest dates from a peach segregating population to identify regulatory candidate genes controlling fruit quality traits at the harvest stage and validate them in contrasting peach varieties for this trait. We identified 467 and 654 differentially expressed genes for early and late harvest through a transcriptomic approach. In addition, using the Arabidopsis DAP-seq database and network analysis, six transcription factors were selected. Our results suggest significant hormonal balance and cell wall composition/structure differences between early and late harvest samples. Thus, we propose that higher expression levels of the transcription factors <i>HB7</i>, <i>ERF017</i> and <i>WRKY70</i> in early harvest individuals would induce the expression of genes associated with the jasmonic acid pathway, photosynthesis and gibberellins inhibition. While on the other hand, the high expression levels of <i>LHY</i>, <i>CDF3</i> and <i>NAC083</i> in late harvest individuals would promote the induction of genes associated with abscisic acid biosynthesis, auxins and cell wall remodeling.https://www.mdpi.com/2223-7747/11/24/3473fruit ripeningtranscriptomicsnetwork analysistranscription factorspeach development |
| spellingShingle | Gerardo Núñez-Lillo Wellasmin Pérez-Reyes Anibal Riveros Victoria Lillo-Carmona Karin Rothkegel José Miguel Álvarez Francisca Blanco-Herrera Romina Pedreschi Reinaldo Campos-Vargas Claudio Meneses Transcriptome and Gene Regulatory Network Analyses Reveal New Transcription Factors in Mature Fruit Associated with Harvest Date in <i>Prunus persica</i> Plants fruit ripening transcriptomics network analysis transcription factors peach development |
| title | Transcriptome and Gene Regulatory Network Analyses Reveal New Transcription Factors in Mature Fruit Associated with Harvest Date in <i>Prunus persica</i> |
| title_full | Transcriptome and Gene Regulatory Network Analyses Reveal New Transcription Factors in Mature Fruit Associated with Harvest Date in <i>Prunus persica</i> |
| title_fullStr | Transcriptome and Gene Regulatory Network Analyses Reveal New Transcription Factors in Mature Fruit Associated with Harvest Date in <i>Prunus persica</i> |
| title_full_unstemmed | Transcriptome and Gene Regulatory Network Analyses Reveal New Transcription Factors in Mature Fruit Associated with Harvest Date in <i>Prunus persica</i> |
| title_short | Transcriptome and Gene Regulatory Network Analyses Reveal New Transcription Factors in Mature Fruit Associated with Harvest Date in <i>Prunus persica</i> |
| title_sort | transcriptome and gene regulatory network analyses reveal new transcription factors in mature fruit associated with harvest date in i prunus persica i |
| topic | fruit ripening transcriptomics network analysis transcription factors peach development |
| url | https://www.mdpi.com/2223-7747/11/24/3473 |
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