Viral evasion of a bacterial suicide system by RNA-based molecular mimicry enables infectious altruism.
Abortive infection, during which an infected bacterial cell commits altruistic suicide to destroy the replicating bacteriophage and protect the clonal population, can be mediated by toxin-antitoxin systems such as the Type III protein-RNA toxin-antitoxin system, ToxIN. A flagellum-dependent bacterio...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS Genetics |
| Online Access: | http://europepmc.org/articles/PMC3475682?pdf=render |
| _version_ | 1811276208519774208 |
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| author | Tim R Blower Terry J Evans Rita Przybilski Peter C Fineran George P C Salmond |
| author_facet | Tim R Blower Terry J Evans Rita Przybilski Peter C Fineran George P C Salmond |
| author_sort | Tim R Blower |
| collection | DOAJ |
| description | Abortive infection, during which an infected bacterial cell commits altruistic suicide to destroy the replicating bacteriophage and protect the clonal population, can be mediated by toxin-antitoxin systems such as the Type III protein-RNA toxin-antitoxin system, ToxIN. A flagellum-dependent bacteriophage of the Myoviridae, ΦTE, evolved rare mutants that "escaped" ToxIN-mediated abortive infection within Pectobacterium atrosepticum. Wild-type ΦTE encoded a short sequence similar to the repetitive nucleotide sequence of the RNA antitoxin, ToxI, from ToxIN. The ΦTE escape mutants had expanded the number of these "pseudo-ToxI" genetic repeats and, in one case, an escape phage had "hijacked" ToxI from the plasmid-borne toxIN locus, through recombination. Expression of the pseudo-ToxI repeats during ΦTE infection allowed the phage to replicate, unaffected by ToxIN, through RNA-based molecular mimicry. This is the first example of a non-coding RNA encoded by a phage that evolves by selective expansion and recombination to enable viral suppression of a defensive bacterial suicide system. Furthermore, the ΦTE escape phages had evolved enhanced capacity to transduce replicons expressing ToxIN, demonstrating virus-mediated horizontal transfer of genetic altruism. |
| first_indexed | 2024-04-12T23:52:23Z |
| format | Article |
| id | doaj.art-468f23a15a284ad09e36108e06a7bed6 |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-04-12T23:52:23Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-468f23a15a284ad09e36108e06a7bed62022-12-22T03:11:38ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042012-01-01810e100302310.1371/journal.pgen.1003023Viral evasion of a bacterial suicide system by RNA-based molecular mimicry enables infectious altruism.Tim R BlowerTerry J EvansRita PrzybilskiPeter C FineranGeorge P C SalmondAbortive infection, during which an infected bacterial cell commits altruistic suicide to destroy the replicating bacteriophage and protect the clonal population, can be mediated by toxin-antitoxin systems such as the Type III protein-RNA toxin-antitoxin system, ToxIN. A flagellum-dependent bacteriophage of the Myoviridae, ΦTE, evolved rare mutants that "escaped" ToxIN-mediated abortive infection within Pectobacterium atrosepticum. Wild-type ΦTE encoded a short sequence similar to the repetitive nucleotide sequence of the RNA antitoxin, ToxI, from ToxIN. The ΦTE escape mutants had expanded the number of these "pseudo-ToxI" genetic repeats and, in one case, an escape phage had "hijacked" ToxI from the plasmid-borne toxIN locus, through recombination. Expression of the pseudo-ToxI repeats during ΦTE infection allowed the phage to replicate, unaffected by ToxIN, through RNA-based molecular mimicry. This is the first example of a non-coding RNA encoded by a phage that evolves by selective expansion and recombination to enable viral suppression of a defensive bacterial suicide system. Furthermore, the ΦTE escape phages had evolved enhanced capacity to transduce replicons expressing ToxIN, demonstrating virus-mediated horizontal transfer of genetic altruism.http://europepmc.org/articles/PMC3475682?pdf=render |
| spellingShingle | Tim R Blower Terry J Evans Rita Przybilski Peter C Fineran George P C Salmond Viral evasion of a bacterial suicide system by RNA-based molecular mimicry enables infectious altruism. PLoS Genetics |
| title | Viral evasion of a bacterial suicide system by RNA-based molecular mimicry enables infectious altruism. |
| title_full | Viral evasion of a bacterial suicide system by RNA-based molecular mimicry enables infectious altruism. |
| title_fullStr | Viral evasion of a bacterial suicide system by RNA-based molecular mimicry enables infectious altruism. |
| title_full_unstemmed | Viral evasion of a bacterial suicide system by RNA-based molecular mimicry enables infectious altruism. |
| title_short | Viral evasion of a bacterial suicide system by RNA-based molecular mimicry enables infectious altruism. |
| title_sort | viral evasion of a bacterial suicide system by rna based molecular mimicry enables infectious altruism |
| url | http://europepmc.org/articles/PMC3475682?pdf=render |
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