Interactions of <i>Bacillus subtilis</i> Basement Spore Coat Layer Proteins
<i>Bacillus subtilis</i> endospores are exceptionally resistant cells encircled by two protective layers: a petidoglycan layer, termed the cortex, and the spore coat, a proteinaceous layer. The formation of both structures depends upon the proper assembly of a basement coat layer, which...
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| Format: | Article |
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MDPI AG
2021-01-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/2/285 |
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| author | Daniela Krajčíková Veronika Bugárová Imrich Barák |
| author_facet | Daniela Krajčíková Veronika Bugárová Imrich Barák |
| author_sort | Daniela Krajčíková |
| collection | DOAJ |
| description | <i>Bacillus subtilis</i> endospores are exceptionally resistant cells encircled by two protective layers: a petidoglycan layer, termed the cortex, and the spore coat, a proteinaceous layer. The formation of both structures depends upon the proper assembly of a basement coat layer, which is composed of two proteins, SpoIVA and SpoVM. The present work examines the interactions of SpoIVA and SpoVM with coat proteins recruited to the spore surface during the early stages of coat assembly. We showed that the alanine racemase YncD associates with two morphogenetic proteins, SpoIVA and CotE. Mutant spores lacking the <i>yncD</i> gene were less resistant against wet heat and germinated to a greater extent than wild-type spores in the presence of micromolar concentrations of <span style="font-variant: small-caps;">l</span>-alanine. In seeking a link between the coat and cortex formation, we investigated the interactions between SpoVM and SpoIVA and the proteins essential for cortex synthesis and found that SpoVM interacts with a penicillin-binding protein, SpoVD, and we also demonstrated that SpoVM is crucial for the proper localization of SpoVD. This study shows that direct contacts between coat morphogenetic proteins with a complex of cortex-synthesizing proteins could be one of the tools by which bacteria couple cortex and coat formation. |
| first_indexed | 2024-03-09T03:15:49Z |
| format | Article |
| id | doaj.art-468f7b18512945239edd03c55e6e564b |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T03:15:49Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-468f7b18512945239edd03c55e6e564b2023-12-03T15:20:12ZengMDPI AGMicroorganisms2076-26072021-01-019228510.3390/microorganisms9020285Interactions of <i>Bacillus subtilis</i> Basement Spore Coat Layer ProteinsDaniela Krajčíková0Veronika Bugárová1Imrich Barák2Department of Microbial Genetics, Institute of Molecular Biology, Slovak Academy of Sciences, Dúbravská cesta 21, 845 51 Bratislava, SlovakiaDepartment of Microbial Genetics, Institute of Molecular Biology, Slovak Academy of Sciences, Dúbravská cesta 21, 845 51 Bratislava, SlovakiaDepartment of Microbial Genetics, Institute of Molecular Biology, Slovak Academy of Sciences, Dúbravská cesta 21, 845 51 Bratislava, Slovakia<i>Bacillus subtilis</i> endospores are exceptionally resistant cells encircled by two protective layers: a petidoglycan layer, termed the cortex, and the spore coat, a proteinaceous layer. The formation of both structures depends upon the proper assembly of a basement coat layer, which is composed of two proteins, SpoIVA and SpoVM. The present work examines the interactions of SpoIVA and SpoVM with coat proteins recruited to the spore surface during the early stages of coat assembly. We showed that the alanine racemase YncD associates with two morphogenetic proteins, SpoIVA and CotE. Mutant spores lacking the <i>yncD</i> gene were less resistant against wet heat and germinated to a greater extent than wild-type spores in the presence of micromolar concentrations of <span style="font-variant: small-caps;">l</span>-alanine. In seeking a link between the coat and cortex formation, we investigated the interactions between SpoVM and SpoIVA and the proteins essential for cortex synthesis and found that SpoVM interacts with a penicillin-binding protein, SpoVD, and we also demonstrated that SpoVM is crucial for the proper localization of SpoVD. This study shows that direct contacts between coat morphogenetic proteins with a complex of cortex-synthesizing proteins could be one of the tools by which bacteria couple cortex and coat formation.https://www.mdpi.com/2076-2607/9/2/285spore coatprotein-protein interactioncortexPBP proteinalanine racemaseSpoVM |
| spellingShingle | Daniela Krajčíková Veronika Bugárová Imrich Barák Interactions of <i>Bacillus subtilis</i> Basement Spore Coat Layer Proteins Microorganisms spore coat protein-protein interaction cortex PBP protein alanine racemase SpoVM |
| title | Interactions of <i>Bacillus subtilis</i> Basement Spore Coat Layer Proteins |
| title_full | Interactions of <i>Bacillus subtilis</i> Basement Spore Coat Layer Proteins |
| title_fullStr | Interactions of <i>Bacillus subtilis</i> Basement Spore Coat Layer Proteins |
| title_full_unstemmed | Interactions of <i>Bacillus subtilis</i> Basement Spore Coat Layer Proteins |
| title_short | Interactions of <i>Bacillus subtilis</i> Basement Spore Coat Layer Proteins |
| title_sort | interactions of i bacillus subtilis i basement spore coat layer proteins |
| topic | spore coat protein-protein interaction cortex PBP protein alanine racemase SpoVM |
| url | https://www.mdpi.com/2076-2607/9/2/285 |
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