Impact of culture vessels on micro-morphological features of in vitro Dendrobium Sabin Blue orchid
Different designs of the plant tissue culture vessel, such as size, material, and shape, may alter its micro environ ment atmosphere. The present study was conducted on protocorm-like bodies (PLBs) of Dendrobium Sabin Blue orchid to determine the development of PLBs on plastic and glass culture vess...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Termedia Publishing House
2022-03-01
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| Series: | BioTechnologia |
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| Online Access: | https://www.termedia.pl/Impact-of-culture-vessels-on-micro-morphological-features-r-nof-in-vitro-Dendrobium-Sabin-Blue-orchid,85,46482,1,1.html |
| _version_ | 1817993680563208192 |
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| author | Aimie Nadzirah Abd Malik Jasim Uddain Chee Keong Chin Bee Lynn Chew Jessica Jeyanthi James Antony Ankita Rajendra Parab Sreeramanan Subramaniam |
| author_facet | Aimie Nadzirah Abd Malik Jasim Uddain Chee Keong Chin Bee Lynn Chew Jessica Jeyanthi James Antony Ankita Rajendra Parab Sreeramanan Subramaniam |
| author_sort | Aimie Nadzirah Abd Malik |
| collection | DOAJ |
| description | Different designs of the plant tissue culture vessel, such as size, material, and shape, may alter its micro environ ment atmosphere. The present study was conducted on protocorm-like bodies (PLBs) of Dendrobium Sabin Blue orchid to determine the development of PLBs on plastic and glass culture vessels of different sizes. PLBs were cultured in half-strength Murashige and Skoog (MS) medium with the same initial weight of 0.5 g in 10 replicates. The growth index of the PLBs was calculated after 11 weeks to study their growth in every vessel; additionally, bio chemical analysis was performed to determine carbohydrate content, proline concentration, and photo synthesis pigments in the PLBs. Scanning electron microscopy (SEM) was performed to study stomata deve lop ment on PLBs in each vessel, and histological analyses were conducted to study the cell structure. Overall, the PLBs cultured in a large 470 ml plastic vessel showed successful growth with a high growth index, high carbo hydrate content, low-stress condition, and high chlorophyll content. SEM confirmed that the presence of trichome and rhizoid in PLBs cultured in the 470 ml plastic vessel. Histological analysis showed the formation of the shoot on the PLBs and the presence of starch granules. Thus, the use of plastic as a culture vessel provides a good impact for culturing PLBs and has low cost. |
| first_indexed | 2024-04-14T01:43:00Z |
| format | Article |
| id | doaj.art-468fa5f77c974c3a8e55550959b2da54 |
| institution | Directory Open Access Journal |
| issn | 0860-7796 2353-9461 |
| language | English |
| last_indexed | 2024-04-14T01:43:00Z |
| publishDate | 2022-03-01 |
| publisher | Termedia Publishing House |
| record_format | Article |
| series | BioTechnologia |
| spelling | doaj.art-468fa5f77c974c3a8e55550959b2da542022-12-22T02:19:39ZengTermedia Publishing HouseBioTechnologia0860-77962353-94612022-03-011031415210.5114/bta.2022.11391446482Impact of culture vessels on micro-morphological features of in vitro Dendrobium Sabin Blue orchidAimie Nadzirah Abd MalikJasim UddainChee Keong ChinBee Lynn ChewJessica Jeyanthi James AntonyAnkita Rajendra ParabSreeramanan SubramaniamDifferent designs of the plant tissue culture vessel, such as size, material, and shape, may alter its micro environ ment atmosphere. The present study was conducted on protocorm-like bodies (PLBs) of Dendrobium Sabin Blue orchid to determine the development of PLBs on plastic and glass culture vessels of different sizes. PLBs were cultured in half-strength Murashige and Skoog (MS) medium with the same initial weight of 0.5 g in 10 replicates. The growth index of the PLBs was calculated after 11 weeks to study their growth in every vessel; additionally, bio chemical analysis was performed to determine carbohydrate content, proline concentration, and photo synthesis pigments in the PLBs. Scanning electron microscopy (SEM) was performed to study stomata deve lop ment on PLBs in each vessel, and histological analyses were conducted to study the cell structure. Overall, the PLBs cultured in a large 470 ml plastic vessel showed successful growth with a high growth index, high carbo hydrate content, low-stress condition, and high chlorophyll content. SEM confirmed that the presence of trichome and rhizoid in PLBs cultured in the 470 ml plastic vessel. Histological analysis showed the formation of the shoot on the PLBs and the presence of starch granules. Thus, the use of plastic as a culture vessel provides a good impact for culturing PLBs and has low cost.https://www.termedia.pl/Impact-of-culture-vessels-on-micro-morphological-features-r-nof-in-vitro-Dendrobium-Sabin-Blue-orchid,85,46482,1,1.htmlalternative culture vessel orchid in vitro culture protocorm-like bodies (plbs) |
| spellingShingle | Aimie Nadzirah Abd Malik Jasim Uddain Chee Keong Chin Bee Lynn Chew Jessica Jeyanthi James Antony Ankita Rajendra Parab Sreeramanan Subramaniam Impact of culture vessels on micro-morphological features of in vitro Dendrobium Sabin Blue orchid BioTechnologia alternative culture vessel orchid in vitro culture protocorm-like bodies (plbs) |
| title | Impact of culture vessels on micro-morphological features
of in vitro Dendrobium Sabin Blue orchid |
| title_full | Impact of culture vessels on micro-morphological features
of in vitro Dendrobium Sabin Blue orchid |
| title_fullStr | Impact of culture vessels on micro-morphological features
of in vitro Dendrobium Sabin Blue orchid |
| title_full_unstemmed | Impact of culture vessels on micro-morphological features
of in vitro Dendrobium Sabin Blue orchid |
| title_short | Impact of culture vessels on micro-morphological features
of in vitro Dendrobium Sabin Blue orchid |
| title_sort | impact of culture vessels on micro morphological features of in vitro dendrobium sabin blue orchid |
| topic | alternative culture vessel orchid in vitro culture protocorm-like bodies (plbs) |
| url | https://www.termedia.pl/Impact-of-culture-vessels-on-micro-morphological-features-r-nof-in-vitro-Dendrobium-Sabin-Blue-orchid,85,46482,1,1.html |
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