Focused low-intensity pulsed ultrasound upregulates Vimentin protein expression to inhibit chondrocyte apoptosis
Objective To investigate the molecular mechanism of focused low-intensity pulsed ultrasound (FLIPUS) to inhibit chondrocyte apoptosis through mechanical effects. Methods Primary chondrocytes were isolated from the knee of suckling C57BL/6J mice with trypsin combined with type Ⅱ collagenase and then...
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| Format: | Article |
| Language: | zho |
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Editorial Office of Journal of Army Medical University
2023-06-01
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| Series: | 陆军军医大学学报 |
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| Online Access: | http://aammt.tmmu.edu.cn/html/202303147.htm |
| _version_ | 1797792147592708096 |
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| author | LI Dongqian YE Haixia YU Lehua JIA Lang |
| author_facet | LI Dongqian YE Haixia YU Lehua JIA Lang |
| author_sort | LI Dongqian |
| collection | DOAJ |
| description | Objective To investigate the molecular mechanism of focused low-intensity pulsed ultrasound (FLIPUS) to inhibit chondrocyte apoptosis through mechanical effects. Methods Primary chondrocytes were isolated from the knee of suckling C57BL/6J mice with trypsin combined with type Ⅱ collagenase and then cultured with IL-1β stimulation to mimic a cellular model of osteoarthritis-like chondrocyte injury. The model was validated and related protein expression changes were detected after different treatment times. The cells were divided into Control group, IL-1β group and IL-1β+FLIPUS group. The apoptotic rate of chondrocytes was detected by flow cytometry, structural distribution of Vimentin was observed with immunofluorescence assay, and expression of Col2α1, MMP13, Bcl-2, Vimentin and p-Tyr397 was measured with Western blotting. Results Primary mouse chondrocytes were isolated and cultured, and simulated to be a cellular model of osteoarthritis-like chondrocyte injury. With elapse of IL-1β treatment time, the protein levels of Col2α1, Vimentin, p-Tyr397, Bcl-2 and p-AKT were decreased (P < 0.05), and those of MMP13, Bax and cleaved-Caspase3 were increased (P < 0.05) compared to the levels in the Control group. FLIPUS led to reduced apoptosis (P < 0.001), decreased MMP13 expression (P < 0.05) and enhanced levels of Col2α1 (P < 0.01), and Bcl-2 and Vimentin and p-Tyr397 (P < 0.05) when compared with the IL-1β group. Vimentin distribution was remodeled, with increased fluorescence intensity. Conclusion FLIPUS up-regulates Vimentin expression, promotes phosphorylation at the Tyr397 locus of FAK, and inhibits apoptosis in chondrocyte.
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| first_indexed | 2024-03-13T02:29:02Z |
| format | Article |
| id | doaj.art-46926b35d47d45daa9b9bfa6d1ecda6c |
| institution | Directory Open Access Journal |
| issn | 2097-0927 |
| language | zho |
| last_indexed | 2024-03-13T02:29:02Z |
| publishDate | 2023-06-01 |
| publisher | Editorial Office of Journal of Army Medical University |
| record_format | Article |
| series | 陆军军医大学学报 |
| spelling | doaj.art-46926b35d47d45daa9b9bfa6d1ecda6c2023-06-30T01:26:09ZzhoEditorial Office of Journal of Army Medical University陆军军医大学学报2097-09272023-06-0145121292130010.16016/j.2097-0927.202303147Focused low-intensity pulsed ultrasound upregulates Vimentin protein expression to inhibit chondrocyte apoptosisLI Dongqian0YE Haixia1YU Lehua2JIA Lang3Department of Rehabilitation Medicine, the Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400010Department of Rehabilitation, Chongqing City Management College, Chongqing, 401331, ChinaDepartment of Rehabilitation Medicine, the Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400010Department of Rehabilitation Medicine, the Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400010 Objective To investigate the molecular mechanism of focused low-intensity pulsed ultrasound (FLIPUS) to inhibit chondrocyte apoptosis through mechanical effects. Methods Primary chondrocytes were isolated from the knee of suckling C57BL/6J mice with trypsin combined with type Ⅱ collagenase and then cultured with IL-1β stimulation to mimic a cellular model of osteoarthritis-like chondrocyte injury. The model was validated and related protein expression changes were detected after different treatment times. The cells were divided into Control group, IL-1β group and IL-1β+FLIPUS group. The apoptotic rate of chondrocytes was detected by flow cytometry, structural distribution of Vimentin was observed with immunofluorescence assay, and expression of Col2α1, MMP13, Bcl-2, Vimentin and p-Tyr397 was measured with Western blotting. Results Primary mouse chondrocytes were isolated and cultured, and simulated to be a cellular model of osteoarthritis-like chondrocyte injury. With elapse of IL-1β treatment time, the protein levels of Col2α1, Vimentin, p-Tyr397, Bcl-2 and p-AKT were decreased (P < 0.05), and those of MMP13, Bax and cleaved-Caspase3 were increased (P < 0.05) compared to the levels in the Control group. FLIPUS led to reduced apoptosis (P < 0.001), decreased MMP13 expression (P < 0.05) and enhanced levels of Col2α1 (P < 0.01), and Bcl-2 and Vimentin and p-Tyr397 (P < 0.05) when compared with the IL-1β group. Vimentin distribution was remodeled, with increased fluorescence intensity. Conclusion FLIPUS up-regulates Vimentin expression, promotes phosphorylation at the Tyr397 locus of FAK, and inhibits apoptosis in chondrocyte. http://aammt.tmmu.edu.cn/html/202303147.htmfocused low-intensity pulsed ultrasoundknee osteoarthritischondrocyte apoptosismechanical transductionvimentinfak |
| spellingShingle | LI Dongqian YE Haixia YU Lehua JIA Lang Focused low-intensity pulsed ultrasound upregulates Vimentin protein expression to inhibit chondrocyte apoptosis 陆军军医大学学报 focused low-intensity pulsed ultrasound knee osteoarthritis chondrocyte apoptosis mechanical transduction vimentin fak |
| title | Focused low-intensity pulsed ultrasound upregulates Vimentin protein expression to inhibit chondrocyte apoptosis |
| title_full | Focused low-intensity pulsed ultrasound upregulates Vimentin protein expression to inhibit chondrocyte apoptosis |
| title_fullStr | Focused low-intensity pulsed ultrasound upregulates Vimentin protein expression to inhibit chondrocyte apoptosis |
| title_full_unstemmed | Focused low-intensity pulsed ultrasound upregulates Vimentin protein expression to inhibit chondrocyte apoptosis |
| title_short | Focused low-intensity pulsed ultrasound upregulates Vimentin protein expression to inhibit chondrocyte apoptosis |
| title_sort | focused low intensity pulsed ultrasound upregulates vimentin protein expression to inhibit chondrocyte apoptosis |
| topic | focused low-intensity pulsed ultrasound knee osteoarthritis chondrocyte apoptosis mechanical transduction vimentin fak |
| url | http://aammt.tmmu.edu.cn/html/202303147.htm |
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