Bacterial Communities in Three Parts of Intestinal Tracts of Carpenter Bees (<i>Xylocopa tenuiscapa</i>)
This study investigated different bacterial communities in three intestinal parts (foregut, midgut and hindgut) of <i>Xylocopa</i><i>tenuiscapa</i> to understand the roles of gut bacteria. Our phylogenetic analysis revealed that <i>X. tenuiscapa</i> is closely rel...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-08-01
|
| Series: | Insects |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-4450/11/8/497 |
| _version_ | 1797560462329511936 |
|---|---|
| author | Phakamas Subta Phongsathon Yodsuwan Rujipas Yongsawas Ammarin In-on Natapot Warrit Somsak Panha Kitiphong Khongphinitbunjong Panuwan Chantawannakul Korrawat Attasopa Terd Disayathanoowat |
| author_facet | Phakamas Subta Phongsathon Yodsuwan Rujipas Yongsawas Ammarin In-on Natapot Warrit Somsak Panha Kitiphong Khongphinitbunjong Panuwan Chantawannakul Korrawat Attasopa Terd Disayathanoowat |
| author_sort | Phakamas Subta |
| collection | DOAJ |
| description | This study investigated different bacterial communities in three intestinal parts (foregut, midgut and hindgut) of <i>Xylocopa</i><i>tenuiscapa</i> to understand the roles of gut bacteria. Our phylogenetic analysis revealed that <i>X. tenuiscapa</i> is closely related to <i>Xylocopa latipes</i>. The 16S rRNA gene in the genomic DNA samples from the gut was examined by illumina (Solexa) and a total of 998 operational taxonomic unit (OTUs) clusters were found. Taxonomic classification identified 16 bacterial phyla and unclassified bacteria. The dominant bacteria taxa in the three parts of <i>X. tenuiscapa</i> gut were Proteobacteria, Firmicutes, Bacteroidetes and Actinobacteria. In the foregut, Lactobacillales and Enterobacteriaceae were predominantly found. The population in the midgut was similar to that in the foregut, with the addition of <i>Gilliamella</i>, which was also abundant. The most dominant bacteria identified in the hindgut were similar to those in the midgut and Lactobacillales, Enterobacteriaceae, <i>Gilliamella</i>, Bifidobacteriaceae and Flavobacteriaceae appeared in abundance. Moreover, our results suggest that a community structure of bacteria in different parts of <i>X. tenuiscapa</i>’s gut may be an important indicator of carpenter bees’ health. This functional study of bacterial communities revealed significant differences among the three intestinal parts and is the first report of the gut bacteria structure in solitary bees. |
| first_indexed | 2024-03-10T17:59:53Z |
| format | Article |
| id | doaj.art-4692e1c3eb8a459db5c6ad842b78de4d |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T17:59:53Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-4692e1c3eb8a459db5c6ad842b78de4d2023-11-20T08:55:02ZengMDPI AGInsects2075-44502020-08-0111849710.3390/insects11080497Bacterial Communities in Three Parts of Intestinal Tracts of Carpenter Bees (<i>Xylocopa tenuiscapa</i>)Phakamas Subta0Phongsathon Yodsuwan1Rujipas Yongsawas2Ammarin In-on3Natapot Warrit4Somsak Panha5Kitiphong Khongphinitbunjong6Panuwan Chantawannakul7Korrawat Attasopa8Terd Disayathanoowat9Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, ThailandBioinformatics & Systems Biology Program, King Mongkut’s University of Technology Thonburi (Bang Khun Thian Campus), Bang Khun Thian, Bangkok 10150, ThailandCenter of Excellence in Entomology, Department of Biology, Faculty of Science, Chulalongkorn University, Bangkok 10330, ThailandDepartment of Biology, Faculty of Science, Chulalongkorn University, Bangkok 10330, ThailandSchool of Science, Mae Fah Luang University, Chiang Rai 57100, ThailandDepartment of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Entomology and Plant Pathology, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, ThailandThis study investigated different bacterial communities in three intestinal parts (foregut, midgut and hindgut) of <i>Xylocopa</i><i>tenuiscapa</i> to understand the roles of gut bacteria. Our phylogenetic analysis revealed that <i>X. tenuiscapa</i> is closely related to <i>Xylocopa latipes</i>. The 16S rRNA gene in the genomic DNA samples from the gut was examined by illumina (Solexa) and a total of 998 operational taxonomic unit (OTUs) clusters were found. Taxonomic classification identified 16 bacterial phyla and unclassified bacteria. The dominant bacteria taxa in the three parts of <i>X. tenuiscapa</i> gut were Proteobacteria, Firmicutes, Bacteroidetes and Actinobacteria. In the foregut, Lactobacillales and Enterobacteriaceae were predominantly found. The population in the midgut was similar to that in the foregut, with the addition of <i>Gilliamella</i>, which was also abundant. The most dominant bacteria identified in the hindgut were similar to those in the midgut and Lactobacillales, Enterobacteriaceae, <i>Gilliamella</i>, Bifidobacteriaceae and Flavobacteriaceae appeared in abundance. Moreover, our results suggest that a community structure of bacteria in different parts of <i>X. tenuiscapa</i>’s gut may be an important indicator of carpenter bees’ health. This functional study of bacterial communities revealed significant differences among the three intestinal parts and is the first report of the gut bacteria structure in solitary bees.https://www.mdpi.com/2075-4450/11/8/497carpenter beesintestinemicrobiotanext-generation sequencing |
| spellingShingle | Phakamas Subta Phongsathon Yodsuwan Rujipas Yongsawas Ammarin In-on Natapot Warrit Somsak Panha Kitiphong Khongphinitbunjong Panuwan Chantawannakul Korrawat Attasopa Terd Disayathanoowat Bacterial Communities in Three Parts of Intestinal Tracts of Carpenter Bees (<i>Xylocopa tenuiscapa</i>) Insects carpenter bees intestine microbiota next-generation sequencing |
| title | Bacterial Communities in Three Parts of Intestinal Tracts of Carpenter Bees (<i>Xylocopa tenuiscapa</i>) |
| title_full | Bacterial Communities in Three Parts of Intestinal Tracts of Carpenter Bees (<i>Xylocopa tenuiscapa</i>) |
| title_fullStr | Bacterial Communities in Three Parts of Intestinal Tracts of Carpenter Bees (<i>Xylocopa tenuiscapa</i>) |
| title_full_unstemmed | Bacterial Communities in Three Parts of Intestinal Tracts of Carpenter Bees (<i>Xylocopa tenuiscapa</i>) |
| title_short | Bacterial Communities in Three Parts of Intestinal Tracts of Carpenter Bees (<i>Xylocopa tenuiscapa</i>) |
| title_sort | bacterial communities in three parts of intestinal tracts of carpenter bees i xylocopa tenuiscapa i |
| topic | carpenter bees intestine microbiota next-generation sequencing |
| url | https://www.mdpi.com/2075-4450/11/8/497 |
| work_keys_str_mv | AT phakamassubta bacterialcommunitiesinthreepartsofintestinaltractsofcarpenterbeesixylocopatenuiscapai AT phongsathonyodsuwan bacterialcommunitiesinthreepartsofintestinaltractsofcarpenterbeesixylocopatenuiscapai AT rujipasyongsawas bacterialcommunitiesinthreepartsofintestinaltractsofcarpenterbeesixylocopatenuiscapai AT ammarininon bacterialcommunitiesinthreepartsofintestinaltractsofcarpenterbeesixylocopatenuiscapai AT natapotwarrit bacterialcommunitiesinthreepartsofintestinaltractsofcarpenterbeesixylocopatenuiscapai AT somsakpanha bacterialcommunitiesinthreepartsofintestinaltractsofcarpenterbeesixylocopatenuiscapai AT kitiphongkhongphinitbunjong bacterialcommunitiesinthreepartsofintestinaltractsofcarpenterbeesixylocopatenuiscapai AT panuwanchantawannakul bacterialcommunitiesinthreepartsofintestinaltractsofcarpenterbeesixylocopatenuiscapai AT korrawatattasopa bacterialcommunitiesinthreepartsofintestinaltractsofcarpenterbeesixylocopatenuiscapai AT terddisayathanoowat bacterialcommunitiesinthreepartsofintestinaltractsofcarpenterbeesixylocopatenuiscapai |