The secreted peptide PIP1 amplifies immunity through receptor-like kinase 7.
In plants, innate immune responses are initiated by plasma membrane-located pattern recognition receptors (PRRs) upon recognition of elicitors, including exogenous pathogen-associated molecular patterns (PAMPs) and endogenous damage-associated molecular patterns (DAMPs). Arabidopsis thaliana produce...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-09-01
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| Series: | PLoS Pathogens |
| Online Access: | http://europepmc.org/articles/PMC4154866?pdf=render |
| _version_ | 1830515288508989440 |
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| author | Shuguo Hou Xin Wang Donghua Chen Xue Yang Mei Wang David Turrà Antonio Di Pietro Wei Zhang |
| author_facet | Shuguo Hou Xin Wang Donghua Chen Xue Yang Mei Wang David Turrà Antonio Di Pietro Wei Zhang |
| author_sort | Shuguo Hou |
| collection | DOAJ |
| description | In plants, innate immune responses are initiated by plasma membrane-located pattern recognition receptors (PRRs) upon recognition of elicitors, including exogenous pathogen-associated molecular patterns (PAMPs) and endogenous damage-associated molecular patterns (DAMPs). Arabidopsis thaliana produces more than 1000 secreted peptide candidates, but it has yet to be established whether any of these act as elicitors. Here we identified an A. thaliana gene family encoding precursors of PAMP-induced secreted peptides (prePIPs) through an in-silico approach. The expression of some members of the family, including prePIP1 and prePIP2, is induced by a variety of pathogens and elicitors. Subcellular localization and proteolytic processing analyses demonstrated that the prePIP1 product is secreted into extracellular spaces where it is cleaved at the C-terminus. Overexpression of prePIP1 and prePIP2, or exogenous application of PIP1 and PIP2 synthetic peptides corresponding to the C-terminal conserved regions in prePIP1 and prePIP2, enhanced immune responses and pathogen resistance in A. thaliana. Genetic and biochemical analyses suggested that the receptor-like kinase 7 (RLK7) functions as a receptor of PIP1. Once perceived by RLK7, PIP1 initiates overlapping and distinct immune signaling responses together with the DAMP PEP1. PIP1 and PEP1 cooperate in amplifying the immune responses triggered by the PAMP flagellin. Collectively, these studies provide significant insights into immune modulation by Arabidopsis endogenous secreted peptides. |
| first_indexed | 2024-12-22T03:20:48Z |
| format | Article |
| id | doaj.art-46953ee169934a27a8b9c3a1ffe6dd0b |
| institution | Directory Open Access Journal |
| issn | 1553-7366 1553-7374 |
| language | English |
| last_indexed | 2024-12-22T03:20:48Z |
| publishDate | 2014-09-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj.art-46953ee169934a27a8b9c3a1ffe6dd0b2022-12-21T18:40:44ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742014-09-01109e100433110.1371/journal.ppat.1004331The secreted peptide PIP1 amplifies immunity through receptor-like kinase 7.Shuguo HouXin WangDonghua ChenXue YangMei WangDavid TurràAntonio Di PietroWei ZhangIn plants, innate immune responses are initiated by plasma membrane-located pattern recognition receptors (PRRs) upon recognition of elicitors, including exogenous pathogen-associated molecular patterns (PAMPs) and endogenous damage-associated molecular patterns (DAMPs). Arabidopsis thaliana produces more than 1000 secreted peptide candidates, but it has yet to be established whether any of these act as elicitors. Here we identified an A. thaliana gene family encoding precursors of PAMP-induced secreted peptides (prePIPs) through an in-silico approach. The expression of some members of the family, including prePIP1 and prePIP2, is induced by a variety of pathogens and elicitors. Subcellular localization and proteolytic processing analyses demonstrated that the prePIP1 product is secreted into extracellular spaces where it is cleaved at the C-terminus. Overexpression of prePIP1 and prePIP2, or exogenous application of PIP1 and PIP2 synthetic peptides corresponding to the C-terminal conserved regions in prePIP1 and prePIP2, enhanced immune responses and pathogen resistance in A. thaliana. Genetic and biochemical analyses suggested that the receptor-like kinase 7 (RLK7) functions as a receptor of PIP1. Once perceived by RLK7, PIP1 initiates overlapping and distinct immune signaling responses together with the DAMP PEP1. PIP1 and PEP1 cooperate in amplifying the immune responses triggered by the PAMP flagellin. Collectively, these studies provide significant insights into immune modulation by Arabidopsis endogenous secreted peptides.http://europepmc.org/articles/PMC4154866?pdf=render |
| spellingShingle | Shuguo Hou Xin Wang Donghua Chen Xue Yang Mei Wang David Turrà Antonio Di Pietro Wei Zhang The secreted peptide PIP1 amplifies immunity through receptor-like kinase 7. PLoS Pathogens |
| title | The secreted peptide PIP1 amplifies immunity through receptor-like kinase 7. |
| title_full | The secreted peptide PIP1 amplifies immunity through receptor-like kinase 7. |
| title_fullStr | The secreted peptide PIP1 amplifies immunity through receptor-like kinase 7. |
| title_full_unstemmed | The secreted peptide PIP1 amplifies immunity through receptor-like kinase 7. |
| title_short | The secreted peptide PIP1 amplifies immunity through receptor-like kinase 7. |
| title_sort | secreted peptide pip1 amplifies immunity through receptor like kinase 7 |
| url | http://europepmc.org/articles/PMC4154866?pdf=render |
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