Biochemical properties of digestive proteases of melon ladybird, Epilachna chrysomelina (Fabricius) (Col.: Coccinellidae)
Melon ladybird, Epilachna chrysomelina (Fabricius) (Col.: Coccinellidae)is a major pest of cucurbitaceous plants by feeding on leaves at larval and adult stages. In this study, biochemical properties of digestive proteases of the melon ladybird were studied in its alimentary canal. Enzyme activity i...
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| Format: | Article |
| Language: | English |
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Entomological Society of Iran
2017-11-01
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| Series: | نامه انجمن حشرهشناسی ایران |
| Subjects: | |
| Online Access: | https://jesi.areeo.ac.ir/article_113584_046c0db6460db958cd3c546120d04d22.pdf |
| _version_ | 1828082852763271168 |
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| author | Moloud Gholamzadeh-Chitgar roohangiz Ghanbarinezhad Mohammad Ghadamyari |
| author_facet | Moloud Gholamzadeh-Chitgar roohangiz Ghanbarinezhad Mohammad Ghadamyari |
| author_sort | Moloud Gholamzadeh-Chitgar |
| collection | DOAJ |
| description | Melon ladybird, Epilachna chrysomelina (Fabricius) (Col.: Coccinellidae)is a major pest of cucurbitaceous plants by feeding on leaves at larval and adult stages. In this study, biochemical properties of digestive proteases of the melon ladybird were studied in its alimentary canal. Enzyme activity in the digestive systems of 2nd, 3rd and 4th larval instars and adults showed that the highest specific enzyme activities occurred in 3rd instar larvae while no significant differences observed in adults. The optimal pH and temperature for protease activity in alimentary canal were found 5 and 30°C, respectively. The inhibitory effect of EDTA, TLCK, TPCK, PMSF and Iodoacetate were determined 44.32, 26.05, 22.27, 19.04 and 18.93% in the gut of E. chrysomelina, respectively. With respect to the highest inhibition rate in proteolytic activity caused by Iodoacetate, cysteine proteinases are considered as the main proteases in the midgut of the melon ladybird. Zymogram pattern in the native gel indicated that the protease enzyme had one isoform in the alimentary canal of the pest. |
| first_indexed | 2024-04-11T03:58:20Z |
| format | Article |
| id | doaj.art-4697592ee28f44baa5ee17a38ef7cebc |
| institution | Directory Open Access Journal |
| issn | 0259-9996 2783-3968 |
| language | English |
| last_indexed | 2024-04-11T03:58:20Z |
| publishDate | 2017-11-01 |
| publisher | Entomological Society of Iran |
| record_format | Article |
| series | نامه انجمن حشرهشناسی ایران |
| spelling | doaj.art-4697592ee28f44baa5ee17a38ef7cebc2023-01-01T23:48:56ZengEntomological Society of Iranنامه انجمن حشرهشناسی ایران0259-99962783-39682017-11-0137329330410.22117/jesi.2017.113584113584Biochemical properties of digestive proteases of melon ladybird, Epilachna chrysomelina (Fabricius) (Col.: Coccinellidae)Moloud Gholamzadeh-Chitgar0roohangiz Ghanbarinezhad1Mohammad Ghadamyari2Plant Protection Research Department, Mazandaran Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Sari, IranFormer student, department of plant protection, faculty of agricultural science, university of guilanDepartment of Plant Protection, faculty of agricultural science, university of guilanMelon ladybird, Epilachna chrysomelina (Fabricius) (Col.: Coccinellidae)is a major pest of cucurbitaceous plants by feeding on leaves at larval and adult stages. In this study, biochemical properties of digestive proteases of the melon ladybird were studied in its alimentary canal. Enzyme activity in the digestive systems of 2nd, 3rd and 4th larval instars and adults showed that the highest specific enzyme activities occurred in 3rd instar larvae while no significant differences observed in adults. The optimal pH and temperature for protease activity in alimentary canal were found 5 and 30°C, respectively. The inhibitory effect of EDTA, TLCK, TPCK, PMSF and Iodoacetate were determined 44.32, 26.05, 22.27, 19.04 and 18.93% in the gut of E. chrysomelina, respectively. With respect to the highest inhibition rate in proteolytic activity caused by Iodoacetate, cysteine proteinases are considered as the main proteases in the midgut of the melon ladybird. Zymogram pattern in the native gel indicated that the protease enzyme had one isoform in the alimentary canal of the pest.https://jesi.areeo.ac.ir/article_113584_046c0db6460db958cd3c546120d04d22.pdfmelon ladybirdinhibitorprotease |
| spellingShingle | Moloud Gholamzadeh-Chitgar roohangiz Ghanbarinezhad Mohammad Ghadamyari Biochemical properties of digestive proteases of melon ladybird, Epilachna chrysomelina (Fabricius) (Col.: Coccinellidae) نامه انجمن حشرهشناسی ایران melon ladybird inhibitor protease |
| title | Biochemical properties of digestive proteases of melon ladybird, Epilachna chrysomelina (Fabricius) (Col.: Coccinellidae) |
| title_full | Biochemical properties of digestive proteases of melon ladybird, Epilachna chrysomelina (Fabricius) (Col.: Coccinellidae) |
| title_fullStr | Biochemical properties of digestive proteases of melon ladybird, Epilachna chrysomelina (Fabricius) (Col.: Coccinellidae) |
| title_full_unstemmed | Biochemical properties of digestive proteases of melon ladybird, Epilachna chrysomelina (Fabricius) (Col.: Coccinellidae) |
| title_short | Biochemical properties of digestive proteases of melon ladybird, Epilachna chrysomelina (Fabricius) (Col.: Coccinellidae) |
| title_sort | biochemical properties of digestive proteases of melon ladybird epilachna chrysomelina fabricius col coccinellidae |
| topic | melon ladybird inhibitor protease |
| url | https://jesi.areeo.ac.ir/article_113584_046c0db6460db958cd3c546120d04d22.pdf |
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