Aristolochic Acid I-Induced Hepatotoxicity in Tianfu Broilers Is Associated with Oxidative-Stress-Mediated Apoptosis and Mitochondrial Damage
Aristolochic acid (AA) is a component of traditional Chinese herbs and commonly used for farm animals in China. Over-exposure of AA has been proven to be associated with hepatotoxicity; however, the mechanism of action of AA-I-induced hepatotoxicity remains unknown. In the current study, a subchroni...
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MDPI AG
2021-12-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/11/12/3437 |
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| author | Dan Xu Lizi Yin Juchun Lin Hualin Fu Xi Peng Lijen Chang Yilei Zheng Xiaoling Zhao Gang Shu |
| author_facet | Dan Xu Lizi Yin Juchun Lin Hualin Fu Xi Peng Lijen Chang Yilei Zheng Xiaoling Zhao Gang Shu |
| author_sort | Dan Xu |
| collection | DOAJ |
| description | Aristolochic acid (AA) is a component of traditional Chinese herbs and commonly used for farm animals in China. Over-exposure of AA has been proven to be associated with hepatotoxicity; however, the mechanism of action of AA-I-induced hepatotoxicity remains unknown. In the current study, a subchronic toxicity test was conducted to evaluate the mechanism of AA-induced hepatotoxicity in Tianfu broilers. According to the results, AA-I-induced hepatotoxicity in Tianfu broilers was evidenced by the elevation of liver weight, levels of serum glutamic oxalacetic transaminase (GOT) and glutamic-pyruvic transaminase (GPT). Furthermore, hepatocyte swelling, vesicular degeneration and steatosis were observed. Additionally, AA-I elevated the production of reactive oxygen species (ROS) and induced oxidative stress, which further led to excessive apoptosis, characterized by mitochondrial depolarization, upregulation of Bax, and down-regulation of Bcl-2 expression. In conclusion, the mechanism of AA-I-induced hepatotoxicity was associated with oxidative-stress-mediated apoptosis and mitochondrial damage. |
| first_indexed | 2024-03-10T04:39:25Z |
| format | Article |
| id | doaj.art-469affef3c2144e7bcaf8384e03c349c |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T04:39:25Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-469affef3c2144e7bcaf8384e03c349c2023-11-23T03:26:56ZengMDPI AGAnimals2076-26152021-12-011112343710.3390/ani11123437Aristolochic Acid I-Induced Hepatotoxicity in Tianfu Broilers Is Associated with Oxidative-Stress-Mediated Apoptosis and Mitochondrial DamageDan Xu0Lizi Yin1Juchun Lin2Hualin Fu3Xi Peng4Lijen Chang5Yilei Zheng6Xiaoling Zhao7Gang Shu8Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, ChinaDepartment of Basic Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaDepartment of Basic Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaDepartment of Basic Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaSichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu 611130, ChinaDepartment of Veterinary Clinical Science, College of Veterinary Medicine, Oklahoma State University, Stillwater, OK 74078, USACollege of Veterinary Medicine, University of Minnesota, Minneapolis, MN 55791, USAFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, ChinaDepartment of Basic Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaAristolochic acid (AA) is a component of traditional Chinese herbs and commonly used for farm animals in China. Over-exposure of AA has been proven to be associated with hepatotoxicity; however, the mechanism of action of AA-I-induced hepatotoxicity remains unknown. In the current study, a subchronic toxicity test was conducted to evaluate the mechanism of AA-induced hepatotoxicity in Tianfu broilers. According to the results, AA-I-induced hepatotoxicity in Tianfu broilers was evidenced by the elevation of liver weight, levels of serum glutamic oxalacetic transaminase (GOT) and glutamic-pyruvic transaminase (GPT). Furthermore, hepatocyte swelling, vesicular degeneration and steatosis were observed. Additionally, AA-I elevated the production of reactive oxygen species (ROS) and induced oxidative stress, which further led to excessive apoptosis, characterized by mitochondrial depolarization, upregulation of Bax, and down-regulation of Bcl-2 expression. In conclusion, the mechanism of AA-I-induced hepatotoxicity was associated with oxidative-stress-mediated apoptosis and mitochondrial damage.https://www.mdpi.com/2076-2615/11/12/3437aristolochic acidliveroxidative stressapoptosismitochondria |
| spellingShingle | Dan Xu Lizi Yin Juchun Lin Hualin Fu Xi Peng Lijen Chang Yilei Zheng Xiaoling Zhao Gang Shu Aristolochic Acid I-Induced Hepatotoxicity in Tianfu Broilers Is Associated with Oxidative-Stress-Mediated Apoptosis and Mitochondrial Damage Animals aristolochic acid liver oxidative stress apoptosis mitochondria |
| title | Aristolochic Acid I-Induced Hepatotoxicity in Tianfu Broilers Is Associated with Oxidative-Stress-Mediated Apoptosis and Mitochondrial Damage |
| title_full | Aristolochic Acid I-Induced Hepatotoxicity in Tianfu Broilers Is Associated with Oxidative-Stress-Mediated Apoptosis and Mitochondrial Damage |
| title_fullStr | Aristolochic Acid I-Induced Hepatotoxicity in Tianfu Broilers Is Associated with Oxidative-Stress-Mediated Apoptosis and Mitochondrial Damage |
| title_full_unstemmed | Aristolochic Acid I-Induced Hepatotoxicity in Tianfu Broilers Is Associated with Oxidative-Stress-Mediated Apoptosis and Mitochondrial Damage |
| title_short | Aristolochic Acid I-Induced Hepatotoxicity in Tianfu Broilers Is Associated with Oxidative-Stress-Mediated Apoptosis and Mitochondrial Damage |
| title_sort | aristolochic acid i induced hepatotoxicity in tianfu broilers is associated with oxidative stress mediated apoptosis and mitochondrial damage |
| topic | aristolochic acid liver oxidative stress apoptosis mitochondria |
| url | https://www.mdpi.com/2076-2615/11/12/3437 |
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