High genetic diversity and mixing of coastal horseshoe crabs (Tachypleus gigas) across major habitats in Sundaland, Indonesia
Species with limited dispersal abilities are often composed of highly genetically structured populations across small geographic ranges. This study aimed to investigate the haplotype diversity and genetic connectivity of the coastal horseshoe crab (Tachypleus gigas) in Indonesia. To achieve this, we...
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PeerJ Inc.
2021-08-01
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| Online Access: | https://peerj.com/articles/11739.pdf |
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| author | Naila Khuril Aini Yusli Wardiatno Hefni Effendi Ali Mashar Hawis Madduppa |
| author_facet | Naila Khuril Aini Yusli Wardiatno Hefni Effendi Ali Mashar Hawis Madduppa |
| author_sort | Naila Khuril Aini |
| collection | DOAJ |
| description | Species with limited dispersal abilities are often composed of highly genetically structured populations across small geographic ranges. This study aimed to investigate the haplotype diversity and genetic connectivity of the coastal horseshoe crab (Tachypleus gigas) in Indonesia. To achieve this, we collected a total of 91 samples from six main T. gigas habitats: Bintan, Balikpapan, Demak, Madura, Subang, and Ujung Kulon. The samples were amplified using primers for mitochondrial (mt) AT-rich region DNA sequences. The results showed 34 haplotypes, including seven shared and 22 unique haplotypes, across all localities. The pairwise genetic differentiation (FST) values were low (0 to 0.13) and not significantly different (p > 0.05), except among samples from Ujung Kulon-Madura and Kulon-Subang (p < 0.05). Additionally, the 34 analysis of molecular variance (AMOVA) showed the most variation within populations (95.23%) compared to less among populations (4.77%). The haplotype network showed evidence of shared haplotypes between populations. Tajima’s D and Fu’s FS test values indicated a population expansion. Our results showed a low level of differentiation, suggesting a single stock and high connectivity. Therefore, a regionally-based conservation strategy is recommended for the coastal horseshoe crab in Indonesia. |
| first_indexed | 2024-03-09T08:06:19Z |
| format | Article |
| id | doaj.art-469c693e236b467aaaaa4abc3cfcdce3 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T08:06:19Z |
| publishDate | 2021-08-01 |
| publisher | PeerJ Inc. |
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| spelling | doaj.art-469c693e236b467aaaaa4abc3cfcdce32023-12-02T23:48:13ZengPeerJ Inc.PeerJ2167-83592021-08-019e1173910.7717/peerj.11739High genetic diversity and mixing of coastal horseshoe crabs (Tachypleus gigas) across major habitats in Sundaland, IndonesiaNaila Khuril Aini0Yusli Wardiatno1Hefni Effendi2Ali Mashar3Hawis Madduppa4Study Program of Aquatic Resources, Department of Aquatic Resources Management, Faculty of Fisheries and Marine Sciences, Graduate School, Institut Pertanian Bogor, Bogor, Jawa Barat, IndonesiaDepartment of Aquatic Resources Management, Faculty of Fisheries and Marine Sciences, Institut Pertanian Bogor, Bogor, Jawa Barat, IndonesiaDepartment of Aquatic Resources Management, Faculty of Fisheries and Marine Sciences, Institut Pertanian Bogor, Bogor, Jawa Barat, IndonesiaDepartment of Aquatic Resources Management, Faculty of Fisheries and Marine Sciences, Institut Pertanian Bogor, Bogor, Jawa Barat, IndonesiaDepartment of Marine Science and Technology, Faculty of Fisheries and Marine Sciences, Institut Pertanian Bogor, Bogor, Jawa Barat, IndonesiaSpecies with limited dispersal abilities are often composed of highly genetically structured populations across small geographic ranges. This study aimed to investigate the haplotype diversity and genetic connectivity of the coastal horseshoe crab (Tachypleus gigas) in Indonesia. To achieve this, we collected a total of 91 samples from six main T. gigas habitats: Bintan, Balikpapan, Demak, Madura, Subang, and Ujung Kulon. The samples were amplified using primers for mitochondrial (mt) AT-rich region DNA sequences. The results showed 34 haplotypes, including seven shared and 22 unique haplotypes, across all localities. The pairwise genetic differentiation (FST) values were low (0 to 0.13) and not significantly different (p > 0.05), except among samples from Ujung Kulon-Madura and Kulon-Subang (p < 0.05). Additionally, the 34 analysis of molecular variance (AMOVA) showed the most variation within populations (95.23%) compared to less among populations (4.77%). The haplotype network showed evidence of shared haplotypes between populations. Tajima’s D and Fu’s FS test values indicated a population expansion. Our results showed a low level of differentiation, suggesting a single stock and high connectivity. Therefore, a regionally-based conservation strategy is recommended for the coastal horseshoe crab in Indonesia.https://peerj.com/articles/11739.pdfPopulation geneticsZoogeographyBiogeographyMolecular biologyEndangered speciesProtected species |
| spellingShingle | Naila Khuril Aini Yusli Wardiatno Hefni Effendi Ali Mashar Hawis Madduppa High genetic diversity and mixing of coastal horseshoe crabs (Tachypleus gigas) across major habitats in Sundaland, Indonesia PeerJ Population genetics Zoogeography Biogeography Molecular biology Endangered species Protected species |
| title | High genetic diversity and mixing of coastal horseshoe crabs (Tachypleus gigas) across major habitats in Sundaland, Indonesia |
| title_full | High genetic diversity and mixing of coastal horseshoe crabs (Tachypleus gigas) across major habitats in Sundaland, Indonesia |
| title_fullStr | High genetic diversity and mixing of coastal horseshoe crabs (Tachypleus gigas) across major habitats in Sundaland, Indonesia |
| title_full_unstemmed | High genetic diversity and mixing of coastal horseshoe crabs (Tachypleus gigas) across major habitats in Sundaland, Indonesia |
| title_short | High genetic diversity and mixing of coastal horseshoe crabs (Tachypleus gigas) across major habitats in Sundaland, Indonesia |
| title_sort | high genetic diversity and mixing of coastal horseshoe crabs tachypleus gigas across major habitats in sundaland indonesia |
| topic | Population genetics Zoogeography Biogeography Molecular biology Endangered species Protected species |
| url | https://peerj.com/articles/11739.pdf |
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