Maternal serum proteomic profiles of pregnant women with type 1 diabetes
Abstract Despite improvement in the care of diabetes over the years, pregnancy complicated by type 1 diabetes (T1DM) is still associated with adverse maternal and neonatal outcomes. To date, proteomics studies have been conducted to identify T1DM biomarkers in non-pregnant women, however, no studies...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-05-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-12221-5 |
| _version_ | 1817989729045446656 |
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| author | Paweł Gutaj Jan Matysiak Eliza Matuszewska Katarzyna Jaskiewicz Dorota Kamińska Agata Światły-Błaszkiewicz Tomasz Szczapa Anastasia Kalantarova Marzena Gajecka Ewa Wender-Ozegowska |
| author_facet | Paweł Gutaj Jan Matysiak Eliza Matuszewska Katarzyna Jaskiewicz Dorota Kamińska Agata Światły-Błaszkiewicz Tomasz Szczapa Anastasia Kalantarova Marzena Gajecka Ewa Wender-Ozegowska |
| author_sort | Paweł Gutaj |
| collection | DOAJ |
| description | Abstract Despite improvement in the care of diabetes over the years, pregnancy complicated by type 1 diabetes (T1DM) is still associated with adverse maternal and neonatal outcomes. To date, proteomics studies have been conducted to identify T1DM biomarkers in non-pregnant women, however, no studies included T1DM pregnant women. In this study serum proteomic profiling was conducted in pregnant women with T1DM in the late third trimester. Serum samples were collected from 40 women with T1DM and 38 healthy controls within 3 days before delivery at term pregnancy. Significant differences between serum proteomic patterns were revealed, showing discriminative peaks for complement C3 and C4-A, kininogen-1, and fibrinogen alpha chain. Quantification of selected discriminative proteins by ELISA kits was also performed. The serum concentration of kininogen-1 was significantly lower in women with T1DM than in controls. There were no significant differences in serum concentrations of complement C3 and complement C4-A between study groups. These data indicate that pregnant women with T1DM have a distinct proteomic profile involving proteins in the coagulation and inflammatory pathways. However, their utility as biomarkers of pregnancy complications in women with T1DM warrants further investigation. |
| first_indexed | 2024-04-14T00:50:35Z |
| format | Article |
| id | doaj.art-469cfab2fa7f488cb74843eba13b80e0 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-14T00:50:35Z |
| publishDate | 2022-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-469cfab2fa7f488cb74843eba13b80e02022-12-22T02:21:49ZengNature PortfolioScientific Reports2045-23222022-05-0112111110.1038/s41598-022-12221-5Maternal serum proteomic profiles of pregnant women with type 1 diabetesPaweł Gutaj0Jan Matysiak1Eliza Matuszewska2Katarzyna Jaskiewicz3Dorota Kamińska4Agata Światły-Błaszkiewicz5Tomasz Szczapa6Anastasia Kalantarova7Marzena Gajecka8Ewa Wender-Ozegowska9Department of Reproduction, Poznan University of Medical SciencesDepartment of Inorganic and Analytical Chemistry, Poznan University of Medical SciencesDepartment of Inorganic and Analytical Chemistry, Poznan University of Medical SciencesChair and Department of Genetics and Pharmaceutical Microbiology, Poznan University of Medical SciencesChair and Department of Genetics and Pharmaceutical Microbiology, Poznan University of Medical SciencesDepartment of Inorganic and Analytical Chemistry, Faculty of Pharmacy, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in ToruńDepartment of Neonatology, Neonatal Biophysical Monitoring and Cardiopulmonary Therapies Research Unit, Poznan University of Medical SciencesMedicine Program, Poznan University of Medical SciencesChair and Department of Genetics and Pharmaceutical Microbiology, Poznan University of Medical SciencesDepartment of Reproduction, Poznan University of Medical SciencesAbstract Despite improvement in the care of diabetes over the years, pregnancy complicated by type 1 diabetes (T1DM) is still associated with adverse maternal and neonatal outcomes. To date, proteomics studies have been conducted to identify T1DM biomarkers in non-pregnant women, however, no studies included T1DM pregnant women. In this study serum proteomic profiling was conducted in pregnant women with T1DM in the late third trimester. Serum samples were collected from 40 women with T1DM and 38 healthy controls within 3 days before delivery at term pregnancy. Significant differences between serum proteomic patterns were revealed, showing discriminative peaks for complement C3 and C4-A, kininogen-1, and fibrinogen alpha chain. Quantification of selected discriminative proteins by ELISA kits was also performed. The serum concentration of kininogen-1 was significantly lower in women with T1DM than in controls. There were no significant differences in serum concentrations of complement C3 and complement C4-A between study groups. These data indicate that pregnant women with T1DM have a distinct proteomic profile involving proteins in the coagulation and inflammatory pathways. However, their utility as biomarkers of pregnancy complications in women with T1DM warrants further investigation.https://doi.org/10.1038/s41598-022-12221-5 |
| spellingShingle | Paweł Gutaj Jan Matysiak Eliza Matuszewska Katarzyna Jaskiewicz Dorota Kamińska Agata Światły-Błaszkiewicz Tomasz Szczapa Anastasia Kalantarova Marzena Gajecka Ewa Wender-Ozegowska Maternal serum proteomic profiles of pregnant women with type 1 diabetes Scientific Reports |
| title | Maternal serum proteomic profiles of pregnant women with type 1 diabetes |
| title_full | Maternal serum proteomic profiles of pregnant women with type 1 diabetes |
| title_fullStr | Maternal serum proteomic profiles of pregnant women with type 1 diabetes |
| title_full_unstemmed | Maternal serum proteomic profiles of pregnant women with type 1 diabetes |
| title_short | Maternal serum proteomic profiles of pregnant women with type 1 diabetes |
| title_sort | maternal serum proteomic profiles of pregnant women with type 1 diabetes |
| url | https://doi.org/10.1038/s41598-022-12221-5 |
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