Regenerated silk fibroin based on small aperture scaffolds and marginal sealing hydrogel for osteochondral defect repair
Abstract Background Osteochondral defects pose an enormous challenge without satisfactory repair strategy to date. In particular, the lateral integration of neo-cartilage into the surrounding native cartilage is a difficult and inadequately addressed problem determining tissue repair’s success. Meth...
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| Format: | Article |
| Language: | English |
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American Association for the Advancement of Science (AAAS)
2023-05-01
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| Series: | Biomaterials Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40824-023-00370-1 |
| _version_ | 1827332293329420288 |
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| author | Yinyue Luo Menglin Xiao Bushra sufyan Almaqrami Hong Kang Zhengzhong Shao Xin Chen Ying Zhang |
| author_facet | Yinyue Luo Menglin Xiao Bushra sufyan Almaqrami Hong Kang Zhengzhong Shao Xin Chen Ying Zhang |
| author_sort | Yinyue Luo |
| collection | DOAJ |
| description | Abstract Background Osteochondral defects pose an enormous challenge without satisfactory repair strategy to date. In particular, the lateral integration of neo-cartilage into the surrounding native cartilage is a difficult and inadequately addressed problem determining tissue repair’s success. Methods Regenerated silk fibroin (RSF) based on small aperture scaffolds was prepared with n-butanol innovatively. Then, the rabbit knee chondrocytes and bone mesenchymal stem cells (BMSCs) were cultured on RSF scaffolds, and after induction of chondrogenic differentiation, cell-scaffold complexes strengthened by a 14 wt% RSF solution were prepared for in vivo experiments. Results A porous scaffold and an RSF sealant exhibiting biocompatibility and excellent adhesive properties are developed and confirmed to promote chondrocyte migration and differentiation. Thus, osteochondral repair and superior horizontal integration are achieved in vivo with this composite. Conclusions Overall, the new approach of marginal sealing around the RSF scaffolds exhibits preeminent repair results, confirming the ability of this novel graft to facilitate simultaneous regeneration of cartilage–subchondral bone. |
| first_indexed | 2024-03-07T16:56:57Z |
| format | Article |
| id | doaj.art-46a1ab8c27194779b74168fbf6b3821a |
| institution | Directory Open Access Journal |
| issn | 2055-7124 |
| language | English |
| last_indexed | 2024-03-07T16:56:57Z |
| publishDate | 2023-05-01 |
| publisher | American Association for the Advancement of Science (AAAS) |
| record_format | Article |
| series | Biomaterials Research |
| spelling | doaj.art-46a1ab8c27194779b74168fbf6b3821a2024-03-03T03:44:50ZengAmerican Association for the Advancement of Science (AAAS)Biomaterials Research2055-71242023-05-0127111710.1186/s40824-023-00370-1Regenerated silk fibroin based on small aperture scaffolds and marginal sealing hydrogel for osteochondral defect repairYinyue Luo0Menglin Xiao1Bushra sufyan Almaqrami2Hong Kang3Zhengzhong Shao4Xin Chen5Ying Zhang6Department of Preventive Dentistry, Shanghai Stomatological Hospital & School of Stomatology, Department of Macromolecular Science, Fudan UniversityDepartment of Preventive Dentistry, Shanghai Stomatological Hospital & School of Stomatology, Department of Macromolecular Science, Fudan UniversityDepartment of Orthodontics, Ningbo Dental HospitalDepartment of Temporomandibular Joint and Occlusion, School/Hospital of Stomatology, Lanzhou UniversityDepartment of Preventive Dentistry, Shanghai Stomatological Hospital & School of Stomatology, Department of Macromolecular Science, Fudan UniversityDepartment of Preventive Dentistry, Shanghai Stomatological Hospital & School of Stomatology, Department of Macromolecular Science, Fudan UniversityDepartment of Preventive Dentistry, Shanghai Stomatological Hospital & School of Stomatology, Department of Macromolecular Science, Fudan UniversityAbstract Background Osteochondral defects pose an enormous challenge without satisfactory repair strategy to date. In particular, the lateral integration of neo-cartilage into the surrounding native cartilage is a difficult and inadequately addressed problem determining tissue repair’s success. Methods Regenerated silk fibroin (RSF) based on small aperture scaffolds was prepared with n-butanol innovatively. Then, the rabbit knee chondrocytes and bone mesenchymal stem cells (BMSCs) were cultured on RSF scaffolds, and after induction of chondrogenic differentiation, cell-scaffold complexes strengthened by a 14 wt% RSF solution were prepared for in vivo experiments. Results A porous scaffold and an RSF sealant exhibiting biocompatibility and excellent adhesive properties are developed and confirmed to promote chondrocyte migration and differentiation. Thus, osteochondral repair and superior horizontal integration are achieved in vivo with this composite. Conclusions Overall, the new approach of marginal sealing around the RSF scaffolds exhibits preeminent repair results, confirming the ability of this novel graft to facilitate simultaneous regeneration of cartilage–subchondral bone.https://doi.org/10.1186/s40824-023-00370-1n-butanolSmall aperture scaffoldsRegenerated silk fibroin hydrogelOsteochondral defects |
| spellingShingle | Yinyue Luo Menglin Xiao Bushra sufyan Almaqrami Hong Kang Zhengzhong Shao Xin Chen Ying Zhang Regenerated silk fibroin based on small aperture scaffolds and marginal sealing hydrogel for osteochondral defect repair Biomaterials Research n-butanol Small aperture scaffolds Regenerated silk fibroin hydrogel Osteochondral defects |
| title | Regenerated silk fibroin based on small aperture scaffolds and marginal sealing hydrogel for osteochondral defect repair |
| title_full | Regenerated silk fibroin based on small aperture scaffolds and marginal sealing hydrogel for osteochondral defect repair |
| title_fullStr | Regenerated silk fibroin based on small aperture scaffolds and marginal sealing hydrogel for osteochondral defect repair |
| title_full_unstemmed | Regenerated silk fibroin based on small aperture scaffolds and marginal sealing hydrogel for osteochondral defect repair |
| title_short | Regenerated silk fibroin based on small aperture scaffolds and marginal sealing hydrogel for osteochondral defect repair |
| title_sort | regenerated silk fibroin based on small aperture scaffolds and marginal sealing hydrogel for osteochondral defect repair |
| topic | n-butanol Small aperture scaffolds Regenerated silk fibroin hydrogel Osteochondral defects |
| url | https://doi.org/10.1186/s40824-023-00370-1 |
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