Pterostilbene and treadmill exercise induce white adipose browning in rats
Objective To investigate the effects of pterostilbene (PST) intervention and treadmill exercise on browning of white adipose in rats and explore the molecular mechanisms. Methods Twenty-four 8-week-old male SD rats were randomly divided into 4 groups: sedentary control group (C), 50 mg/kg PST treatm...
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| Format: | Article |
| Language: | zho |
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Editorial Office of Journal of Third Military Medical University
2020-02-01
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| Series: | Di-san junyi daxue xuebao |
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| Online Access: | http://aammt.tmmu.edu.cn/Upload/rhtml/201910115.htm |
| _version_ | 1818866025400107008 |
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| author | ZHENG Jiawei LIU Wujian ZHU Jundong MI Mantian |
| author_facet | ZHENG Jiawei LIU Wujian ZHU Jundong MI Mantian |
| author_sort | ZHENG Jiawei |
| collection | DOAJ |
| description | Objective To investigate the effects of pterostilbene (PST) intervention and treadmill exercise on browning of white adipose in rats and explore the molecular mechanisms. Methods Twenty-four 8-week-old male SD rats were randomly divided into 4 groups: sedentary control group (C), 50 mg/kg PST treatment group, treadmill exercise (EX) group, and treadmill exercise with PST treatment (EX+PST) group. After corresponding treatments for 4 consecutive weeks, the rats were evaluated for changes in food intake, body weight and inguinal white adipose tissue (iWAT) mass. The morphological features of the iWAT were analyzed with HE staining. The mRNA and protein levels of the marker genes and the regulatory molecules for white fat browning in the iWAT were determined using real-time quantitative PCR and Western blotting, respectively. Results The body weight gain and iWAT index in the 3 treatment groups were all significantly lower than those of the control group (P < 0.05), while food intake did not differ significantly among the 4 groups. Compared with those in the control group, the adipocytes within the iWAT in the 3 treatment groups showed a smaller size and a more spherical morphology accompanied by the presence of cellular compartmentation, which were all characteristics of brown adipocytes. The mRNA and protein levels of the marker genes and regulatory molecules for white adipose browning, including uncoupling protein 1 (UCP1), cell death-inducing DNA fragmentation factor alpha-like effector A (CIDEA), cluster of differentiation 137 (CD137), transmembrane protein 26 (TMEM26), carnitine palmitoyltransferase 1A (CPT1A), cytochrome c (Cyto c), silent information regulator 1 (Sirt1), peroxisome proliferator activator receptor gamma coactivator 1 alpha (PGC-1α), PR domain-containing 16 (PRDM16) and phosphorylated adenosine monophosphate activated protein kinase (p-AMPK) were all significantly increased in the iWAT in the 3 treatment groups (P < 0.05). These changes were more obvious in EX+PST group than in EX group and PST group (P < 0.05). Conclusion PST intervention and treadmill exercise, either alone or in combination, can induce the browning of iWAT and reduce fat accumulation and body weight gain in rats through a mechanism possibly involving the activation of AMPK signal transduction pathway. PST combined with treadmill exercise produces more pronounced effects than either PST or treadmill exercise alone |
| first_indexed | 2024-12-19T10:56:53Z |
| format | Article |
| id | doaj.art-46a53a3a84424b68abbc98fbeedfc64c |
| institution | Directory Open Access Journal |
| issn | 1000-5404 |
| language | zho |
| last_indexed | 2024-12-19T10:56:53Z |
| publishDate | 2020-02-01 |
| publisher | Editorial Office of Journal of Third Military Medical University |
| record_format | Article |
| series | Di-san junyi daxue xuebao |
| spelling | doaj.art-46a53a3a84424b68abbc98fbeedfc64c2022-12-21T20:24:47ZzhoEditorial Office of Journal of Third Military Medical UniversityDi-san junyi daxue xuebao1000-54042020-02-0142435737410.16016/j.1000-5404.201910115Pterostilbene and treadmill exercise induce white adipose browning in ratsZHENG Jiawei0LIU Wujian1ZHU Jundong2MI Mantian 3Department of Military Nutrition and Food Hygiene, Chongqing Key Laboratory of Nutrition and Food Safety, Chongqing Center of Medical Nutrition, Faculty of Military Preventive Medicine, Army Medical University (Third Military Medical University), Chongqing, 400038, China Department of Military Nutrition and Food Hygiene, Chongqing Key Laboratory of Nutrition and Food Safety, Chongqing Center of Medical Nutrition, Faculty of Military Preventive Medicine, Army Medical University (Third Military Medical University), Chongqing, 400038, China Department of Military Nutrition and Food Hygiene, Chongqing Key Laboratory of Nutrition and Food Safety, Chongqing Center of Medical Nutrition, Faculty of Military Preventive Medicine, Army Medical University (Third Military Medical University), Chongqing, 400038, China Department of Military Nutrition and Food Hygiene, Chongqing Key Laboratory of Nutrition and Food Safety, Chongqing Center of Medical Nutrition, Faculty of Military Preventive Medicine, Army Medical University (Third Military Medical University), Chongqing, 400038, China Objective To investigate the effects of pterostilbene (PST) intervention and treadmill exercise on browning of white adipose in rats and explore the molecular mechanisms. Methods Twenty-four 8-week-old male SD rats were randomly divided into 4 groups: sedentary control group (C), 50 mg/kg PST treatment group, treadmill exercise (EX) group, and treadmill exercise with PST treatment (EX+PST) group. After corresponding treatments for 4 consecutive weeks, the rats were evaluated for changes in food intake, body weight and inguinal white adipose tissue (iWAT) mass. The morphological features of the iWAT were analyzed with HE staining. The mRNA and protein levels of the marker genes and the regulatory molecules for white fat browning in the iWAT were determined using real-time quantitative PCR and Western blotting, respectively. Results The body weight gain and iWAT index in the 3 treatment groups were all significantly lower than those of the control group (P < 0.05), while food intake did not differ significantly among the 4 groups. Compared with those in the control group, the adipocytes within the iWAT in the 3 treatment groups showed a smaller size and a more spherical morphology accompanied by the presence of cellular compartmentation, which were all characteristics of brown adipocytes. The mRNA and protein levels of the marker genes and regulatory molecules for white adipose browning, including uncoupling protein 1 (UCP1), cell death-inducing DNA fragmentation factor alpha-like effector A (CIDEA), cluster of differentiation 137 (CD137), transmembrane protein 26 (TMEM26), carnitine palmitoyltransferase 1A (CPT1A), cytochrome c (Cyto c), silent information regulator 1 (Sirt1), peroxisome proliferator activator receptor gamma coactivator 1 alpha (PGC-1α), PR domain-containing 16 (PRDM16) and phosphorylated adenosine monophosphate activated protein kinase (p-AMPK) were all significantly increased in the iWAT in the 3 treatment groups (P < 0.05). These changes were more obvious in EX+PST group than in EX group and PST group (P < 0.05). Conclusion PST intervention and treadmill exercise, either alone or in combination, can induce the browning of iWAT and reduce fat accumulation and body weight gain in rats through a mechanism possibly involving the activation of AMPK signal transduction pathway. PST combined with treadmill exercise produces more pronounced effects than either PST or treadmill exercise alonehttp://aammt.tmmu.edu.cn/Upload/rhtml/201910115.htmpterostilbenetreadmill exercisewhite adipose browninguncoupling protein 1adenosine monophosphateactivated protein kinase |
| spellingShingle | ZHENG Jiawei LIU Wujian ZHU Jundong MI Mantian Pterostilbene and treadmill exercise induce white adipose browning in rats Di-san junyi daxue xuebao pterostilbene treadmill exercise white adipose browning uncoupling protein 1 adenosine monophosphate activated protein kinase |
| title | Pterostilbene and treadmill exercise induce white adipose browning in rats |
| title_full | Pterostilbene and treadmill exercise induce white adipose browning in rats |
| title_fullStr | Pterostilbene and treadmill exercise induce white adipose browning in rats |
| title_full_unstemmed | Pterostilbene and treadmill exercise induce white adipose browning in rats |
| title_short | Pterostilbene and treadmill exercise induce white adipose browning in rats |
| title_sort | pterostilbene and treadmill exercise induce white adipose browning in rats |
| topic | pterostilbene treadmill exercise white adipose browning uncoupling protein 1 adenosine monophosphate activated protein kinase |
| url | http://aammt.tmmu.edu.cn/Upload/rhtml/201910115.htm |
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