Differential effects of coconut versus soy oil on gut microbiota composition and predicted metabolic function in adult mice
Abstract Background Animal studies show that high fat (HF) diet-induced gut microbiota contributes to the development of obesity. Oil composition of high-fat diet affects metabolic inflammation differently with deleterious effects by saturated fat. The aim of the present study was to examine the div...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2018-11-01
|
| Series: | BMC Genomics |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12864-018-5202-z |
| _version_ | 1828230158786494464 |
|---|---|
| author | Vania Patrone Andrea Minuti Michela Lizier Francesco Miragoli Franco Lucchini Erminio Trevisi Filippo Rossi Maria Luisa Callegari |
| author_facet | Vania Patrone Andrea Minuti Michela Lizier Francesco Miragoli Franco Lucchini Erminio Trevisi Filippo Rossi Maria Luisa Callegari |
| author_sort | Vania Patrone |
| collection | DOAJ |
| description | Abstract Background Animal studies show that high fat (HF) diet-induced gut microbiota contributes to the development of obesity. Oil composition of high-fat diet affects metabolic inflammation differently with deleterious effects by saturated fat. The aim of the present study was to examine the diversity and metabolic capacity of the cecal bacterial community in C57BL/6 N mice administered two different diets, enriched respectively with coconut oil (HFC, high in saturated fat) or soy oil (HFS, high in polyunsaturated fat). The relative impact of each hypercaloric diet was evaluated after 2 and 8 weeks of feeding, and compared with that of a low-fat, control diet (LF). Results The HFC diet induced the same body weight gain and fat storage as the HFS diet, but produced higher plasma cholesterol levels after 8 weeks of treatment. At the same time point, the cecal microbiota of HFC diet-fed mice was characterized by an increased relative abundance of Allobaculum, Anaerofustis, F16, Lactobacillus reuteri and Deltaproteobacteria, and a decreased relative abundance of Akkermansia muciniphila compared to HFS mice. Comparison of cecal microbiota of high-fat fed mice versus control mice indicated major changes that were shared between the HFC and the HFS diet, including the increase in Lactobacillus plantarum, Lutispora, and Syntrophomonas, while some other shifts were specifically associated to either coconut or soy oil. Prediction of bacterial gene functions showed that the cecal microbiota of HFC mice was depleted of pathways involved in fatty acid metabolism, amino acid metabolism, xenobiotic degradation and metabolism of terpenoids and polyketides compared to mice on HFS diet. Correlation analysis revealed remarkable relationships between compositional changes in the cecal microbiota and alterations in the metabolic and transcriptomic phenotypes of high-fat fed mice. Conclusions The study highlights significant differences in cecal microbiota composition and predictive functions of mice consuming a diet enriched in coconut vs soy oil. The correlations established between specific bacterial taxa and various traits linked to host lipid metabolism and energy storage give insights into the role and functioning of the gut microbiota that may contribute to diet-induced metabolic disorders. |
| first_indexed | 2024-04-12T18:44:08Z |
| format | Article |
| id | doaj.art-46a5d40b974b45f781363d26e31265e7 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-04-12T18:44:08Z |
| publishDate | 2018-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-46a5d40b974b45f781363d26e31265e72022-12-22T03:20:40ZengBMCBMC Genomics1471-21642018-11-0119111710.1186/s12864-018-5202-zDifferential effects of coconut versus soy oil on gut microbiota composition and predicted metabolic function in adult miceVania Patrone0Andrea Minuti1Michela Lizier2Francesco Miragoli3Franco Lucchini4Erminio Trevisi5Filippo Rossi6Maria Luisa Callegari7Department for Sustainable Food Process (DiSTAS), Università Cattolica del Sacro CuoreDepartment of Animal Sciences, Food and Nutrition (DIANA), Università Cattolica del Sacro CuoreMilan Unit, Istituto di Ricerca Genetica e Biomedica, CNRBiotechnological Research Centre, Università Cattolica del Sacro CuoreBiotechnological Research Centre, Università Cattolica del Sacro CuoreDepartment of Animal Sciences, Food and Nutrition (DIANA), Università Cattolica del Sacro CuoreDepartment of Animal Sciences, Food and Nutrition (DIANA), Università Cattolica del Sacro CuoreBiotechnological Research Centre, Università Cattolica del Sacro CuoreAbstract Background Animal studies show that high fat (HF) diet-induced gut microbiota contributes to the development of obesity. Oil composition of high-fat diet affects metabolic inflammation differently with deleterious effects by saturated fat. The aim of the present study was to examine the diversity and metabolic capacity of the cecal bacterial community in C57BL/6 N mice administered two different diets, enriched respectively with coconut oil (HFC, high in saturated fat) or soy oil (HFS, high in polyunsaturated fat). The relative impact of each hypercaloric diet was evaluated after 2 and 8 weeks of feeding, and compared with that of a low-fat, control diet (LF). Results The HFC diet induced the same body weight gain and fat storage as the HFS diet, but produced higher plasma cholesterol levels after 8 weeks of treatment. At the same time point, the cecal microbiota of HFC diet-fed mice was characterized by an increased relative abundance of Allobaculum, Anaerofustis, F16, Lactobacillus reuteri and Deltaproteobacteria, and a decreased relative abundance of Akkermansia muciniphila compared to HFS mice. Comparison of cecal microbiota of high-fat fed mice versus control mice indicated major changes that were shared between the HFC and the HFS diet, including the increase in Lactobacillus plantarum, Lutispora, and Syntrophomonas, while some other shifts were specifically associated to either coconut or soy oil. Prediction of bacterial gene functions showed that the cecal microbiota of HFC mice was depleted of pathways involved in fatty acid metabolism, amino acid metabolism, xenobiotic degradation and metabolism of terpenoids and polyketides compared to mice on HFS diet. Correlation analysis revealed remarkable relationships between compositional changes in the cecal microbiota and alterations in the metabolic and transcriptomic phenotypes of high-fat fed mice. Conclusions The study highlights significant differences in cecal microbiota composition and predictive functions of mice consuming a diet enriched in coconut vs soy oil. The correlations established between specific bacterial taxa and various traits linked to host lipid metabolism and energy storage give insights into the role and functioning of the gut microbiota that may contribute to diet-induced metabolic disorders.http://link.springer.com/article/10.1186/s12864-018-5202-zHigh-fat dietMouseMicrobiotaAdipose tissueObesityReal-time PCR |
| spellingShingle | Vania Patrone Andrea Minuti Michela Lizier Francesco Miragoli Franco Lucchini Erminio Trevisi Filippo Rossi Maria Luisa Callegari Differential effects of coconut versus soy oil on gut microbiota composition and predicted metabolic function in adult mice BMC Genomics High-fat diet Mouse Microbiota Adipose tissue Obesity Real-time PCR |
| title | Differential effects of coconut versus soy oil on gut microbiota composition and predicted metabolic function in adult mice |
| title_full | Differential effects of coconut versus soy oil on gut microbiota composition and predicted metabolic function in adult mice |
| title_fullStr | Differential effects of coconut versus soy oil on gut microbiota composition and predicted metabolic function in adult mice |
| title_full_unstemmed | Differential effects of coconut versus soy oil on gut microbiota composition and predicted metabolic function in adult mice |
| title_short | Differential effects of coconut versus soy oil on gut microbiota composition and predicted metabolic function in adult mice |
| title_sort | differential effects of coconut versus soy oil on gut microbiota composition and predicted metabolic function in adult mice |
| topic | High-fat diet Mouse Microbiota Adipose tissue Obesity Real-time PCR |
| url | http://link.springer.com/article/10.1186/s12864-018-5202-z |
| work_keys_str_mv | AT vaniapatrone differentialeffectsofcoconutversussoyoilongutmicrobiotacompositionandpredictedmetabolicfunctioninadultmice AT andreaminuti differentialeffectsofcoconutversussoyoilongutmicrobiotacompositionandpredictedmetabolicfunctioninadultmice AT michelalizier differentialeffectsofcoconutversussoyoilongutmicrobiotacompositionandpredictedmetabolicfunctioninadultmice AT francescomiragoli differentialeffectsofcoconutversussoyoilongutmicrobiotacompositionandpredictedmetabolicfunctioninadultmice AT francolucchini differentialeffectsofcoconutversussoyoilongutmicrobiotacompositionandpredictedmetabolicfunctioninadultmice AT erminiotrevisi differentialeffectsofcoconutversussoyoilongutmicrobiotacompositionandpredictedmetabolicfunctioninadultmice AT filipporossi differentialeffectsofcoconutversussoyoilongutmicrobiotacompositionandpredictedmetabolicfunctioninadultmice AT marialuisacallegari differentialeffectsofcoconutversussoyoilongutmicrobiotacompositionandpredictedmetabolicfunctioninadultmice |