Study of the relationship between the age of the rams and the quality of their ejaculates obtained outside the breeding season
The present study aims to research the effect of age on the quality of ejaculates of rams from Synthetic population of Bulgarian dairy breed. Rams divided into two age groups were used for the experiment: G1 (2.5 years) versus G2 (6 years). Ejaculates were obtained by the method of artificial vagina...
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| Format: | Article |
| Language: | English |
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Institute for Animal Husbandry, Belgrade
2020-01-01
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| Series: | Biotechnology in Animal Husbandry |
| Subjects: | |
| Online Access: | https://scindeks-clanci.ceon.rs/data/pdf/1450-9156/2020/1450-91562004437A.pdf |
| _version_ | 1818559413254881280 |
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| author | Andreeva Madlena Stefanov Rossen |
| author_facet | Andreeva Madlena Stefanov Rossen |
| author_sort | Andreeva Madlena |
| collection | DOAJ |
| description | The present study aims to research the effect of age on the quality of ejaculates of rams from Synthetic population of Bulgarian dairy breed. Rams divided into two age groups were used for the experiment: G1 (2.5 years) versus G2 (6 years). Ejaculates were obtained by the method of artificial vagina and diluted with medium 6A-G. The tested ejaculates were obtained outside the breeding season. A Sperm Class Analyzer (SCA Microptic, Barcelona, Spain) was used to study sperm motility. The biochemical activity of the enzymes lactate dehydrogenase (LDH) and gamma-glutamyl transferase (GGT) was determined using an automatic Mindrai BA88 spectrophotometer. The activity of the enzymes was studied in sperm plasma, in aqueous and tritonic extract. Significant differences between the groups were found in total sperm motility (P≤0.05) and LDH enzyme activity in sperm plasma (P≤0.01). Regarding the efficiency of the LDH enzyme in aqueous and tritone extract, no significant differences between the groups was found. No significant differences were found between groups for GGT activity, while age had significant impact on LDH enzyme in sperm plasma. |
| first_indexed | 2024-12-14T00:25:11Z |
| format | Article |
| id | doaj.art-46a6be6dc1db4929b838a4a406f5c010 |
| institution | Directory Open Access Journal |
| issn | 1450-9156 2217-7140 |
| language | English |
| last_indexed | 2024-12-14T00:25:11Z |
| publishDate | 2020-01-01 |
| publisher | Institute for Animal Husbandry, Belgrade |
| record_format | Article |
| series | Biotechnology in Animal Husbandry |
| spelling | doaj.art-46a6be6dc1db4929b838a4a406f5c0102022-12-21T23:25:04ZengInstitute for Animal Husbandry, BelgradeBiotechnology in Animal Husbandry1450-91562217-71402020-01-013644374451450-91562004437AStudy of the relationship between the age of the rams and the quality of their ejaculates obtained outside the breeding seasonAndreeva Madlena0Stefanov Rossen1BAS, Institute of Biology and Immunology of Reproduction "Acad. K. Bratanov", Sofia, BulgariaBAS, Institute of Biology and Immunology of Reproduction "Acad. K. Bratanov", Sofia, BulgariaThe present study aims to research the effect of age on the quality of ejaculates of rams from Synthetic population of Bulgarian dairy breed. Rams divided into two age groups were used for the experiment: G1 (2.5 years) versus G2 (6 years). Ejaculates were obtained by the method of artificial vagina and diluted with medium 6A-G. The tested ejaculates were obtained outside the breeding season. A Sperm Class Analyzer (SCA Microptic, Barcelona, Spain) was used to study sperm motility. The biochemical activity of the enzymes lactate dehydrogenase (LDH) and gamma-glutamyl transferase (GGT) was determined using an automatic Mindrai BA88 spectrophotometer. The activity of the enzymes was studied in sperm plasma, in aqueous and tritonic extract. Significant differences between the groups were found in total sperm motility (P≤0.05) and LDH enzyme activity in sperm plasma (P≤0.01). Regarding the efficiency of the LDH enzyme in aqueous and tritone extract, no significant differences between the groups was found. No significant differences were found between groups for GGT activity, while age had significant impact on LDH enzyme in sperm plasma.https://scindeks-clanci.ceon.rs/data/pdf/1450-9156/2020/1450-91562004437A.pdframspermatozoamotilityldhggt |
| spellingShingle | Andreeva Madlena Stefanov Rossen Study of the relationship between the age of the rams and the quality of their ejaculates obtained outside the breeding season Biotechnology in Animal Husbandry ram spermatozoa motility ldh ggt |
| title | Study of the relationship between the age of the rams and the quality of their ejaculates obtained outside the breeding season |
| title_full | Study of the relationship between the age of the rams and the quality of their ejaculates obtained outside the breeding season |
| title_fullStr | Study of the relationship between the age of the rams and the quality of their ejaculates obtained outside the breeding season |
| title_full_unstemmed | Study of the relationship between the age of the rams and the quality of their ejaculates obtained outside the breeding season |
| title_short | Study of the relationship between the age of the rams and the quality of their ejaculates obtained outside the breeding season |
| title_sort | study of the relationship between the age of the rams and the quality of their ejaculates obtained outside the breeding season |
| topic | ram spermatozoa motility ldh ggt |
| url | https://scindeks-clanci.ceon.rs/data/pdf/1450-9156/2020/1450-91562004437A.pdf |
| work_keys_str_mv | AT andreevamadlena studyoftherelationshipbetweentheageoftheramsandthequalityoftheirejaculatesobtainedoutsidethebreedingseason AT stefanovrossen studyoftherelationshipbetweentheageoftheramsandthequalityoftheirejaculatesobtainedoutsidethebreedingseason |