Detection of porcine epidemic diarrhea virus (PEDV) IgG and IgA in muscle tissue exudate (“meat juice”) specimens
Abstract The diagnostic performance of porcine epidemic diarrhea virus (PEDV) IgG and IgA ELISAs was evaluated using paired serum and meat juice samples collected from PEDV-negative (n = 50) and PEDV-inoculated pigs (n = 87). Serum samples were tested by PEDV (IgG, IgA) ELISAs using a procedure perf...
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| Format: | Article |
| Language: | English |
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BMC
2018-12-01
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| Series: | Porcine Health Management |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s40813-018-0107-4 |
| _version_ | 1811319888671670272 |
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| author | Korakrit Poonsuk Ting-Yu Cheng Ju Ji Jeffrey Zimmerman Luis Giménez-Lirola |
| author_facet | Korakrit Poonsuk Ting-Yu Cheng Ju Ji Jeffrey Zimmerman Luis Giménez-Lirola |
| author_sort | Korakrit Poonsuk |
| collection | DOAJ |
| description | Abstract The diagnostic performance of porcine epidemic diarrhea virus (PEDV) IgG and IgA ELISAs was evaluated using paired serum and meat juice samples collected from PEDV-negative (n = 50) and PEDV-inoculated pigs (n = 87). Serum samples were tested by PEDV (IgG, IgA) ELISAs using a procedure performed routinely at the Iowa State University-Veterinary Diagnostic Laboratory (ISU-VDL). Serum samples were tested using PEDV serum IgG and IgA ELISA procedures as routinely performed at the Iowa State University-Veterinary Diagnostic Laboratory (ISU-VDL). Serum samples were diluted 1:50 and conjugate concentrations were 1/20,000 for IgG and 1/3000 for IgA. Meat juice samples were tested using the serum PEDV IgG and IgA ELISAs, with modifications, i.e., meat juice samples were diluted 1:25 and conjugate concentrations were 1/40,000 for IgG and 1/10,000 for IgA. Receiver operator characteristic (ROC) curve analyses were used to estimate diagnostic sensitivities and specificities over a range of sample-to-positive (S/P) cutoffs. Consistent with previous reports, this study showed that the PEDV IgG and IgA meat juice ELISAs provided excellent diagnostic performance and suggest that meat juice recovered from samples collected at slaughter could be used in routine PEDV surveillance. |
| first_indexed | 2024-04-13T12:50:05Z |
| format | Article |
| id | doaj.art-46a7a2772a35440db72b91359bda39d4 |
| institution | Directory Open Access Journal |
| issn | 2055-5660 |
| language | English |
| last_indexed | 2024-04-13T12:50:05Z |
| publishDate | 2018-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Porcine Health Management |
| spelling | doaj.art-46a7a2772a35440db72b91359bda39d42022-12-22T02:46:14ZengBMCPorcine Health Management2055-56602018-12-01411510.1186/s40813-018-0107-4Detection of porcine epidemic diarrhea virus (PEDV) IgG and IgA in muscle tissue exudate (“meat juice”) specimensKorakrit Poonsuk0Ting-Yu Cheng1Ju Ji2Jeffrey Zimmerman3Luis Giménez-Lirola4Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State UniversityDepartment of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State UniversityDepartment of Statistics, College of Liberal Arts and Sciences, Iowa State UniversityDepartment of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State UniversityDepartment of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State UniversityAbstract The diagnostic performance of porcine epidemic diarrhea virus (PEDV) IgG and IgA ELISAs was evaluated using paired serum and meat juice samples collected from PEDV-negative (n = 50) and PEDV-inoculated pigs (n = 87). Serum samples were tested by PEDV (IgG, IgA) ELISAs using a procedure performed routinely at the Iowa State University-Veterinary Diagnostic Laboratory (ISU-VDL). Serum samples were tested using PEDV serum IgG and IgA ELISA procedures as routinely performed at the Iowa State University-Veterinary Diagnostic Laboratory (ISU-VDL). Serum samples were diluted 1:50 and conjugate concentrations were 1/20,000 for IgG and 1/3000 for IgA. Meat juice samples were tested using the serum PEDV IgG and IgA ELISAs, with modifications, i.e., meat juice samples were diluted 1:25 and conjugate concentrations were 1/40,000 for IgG and 1/10,000 for IgA. Receiver operator characteristic (ROC) curve analyses were used to estimate diagnostic sensitivities and specificities over a range of sample-to-positive (S/P) cutoffs. Consistent with previous reports, this study showed that the PEDV IgG and IgA meat juice ELISAs provided excellent diagnostic performance and suggest that meat juice recovered from samples collected at slaughter could be used in routine PEDV surveillance.http://link.springer.com/article/10.1186/s40813-018-0107-4Porcine epidemic diarrhea virus; meat juiceMuscle exudateAntibodyEnzyme linked immunosorbent assay |
| spellingShingle | Korakrit Poonsuk Ting-Yu Cheng Ju Ji Jeffrey Zimmerman Luis Giménez-Lirola Detection of porcine epidemic diarrhea virus (PEDV) IgG and IgA in muscle tissue exudate (“meat juice”) specimens Porcine Health Management Porcine epidemic diarrhea virus; meat juice Muscle exudate Antibody Enzyme linked immunosorbent assay |
| title | Detection of porcine epidemic diarrhea virus (PEDV) IgG and IgA in muscle tissue exudate (“meat juice”) specimens |
| title_full | Detection of porcine epidemic diarrhea virus (PEDV) IgG and IgA in muscle tissue exudate (“meat juice”) specimens |
| title_fullStr | Detection of porcine epidemic diarrhea virus (PEDV) IgG and IgA in muscle tissue exudate (“meat juice”) specimens |
| title_full_unstemmed | Detection of porcine epidemic diarrhea virus (PEDV) IgG and IgA in muscle tissue exudate (“meat juice”) specimens |
| title_short | Detection of porcine epidemic diarrhea virus (PEDV) IgG and IgA in muscle tissue exudate (“meat juice”) specimens |
| title_sort | detection of porcine epidemic diarrhea virus pedv igg and iga in muscle tissue exudate meat juice specimens |
| topic | Porcine epidemic diarrhea virus; meat juice Muscle exudate Antibody Enzyme linked immunosorbent assay |
| url | http://link.springer.com/article/10.1186/s40813-018-0107-4 |
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