Screening of fungi isolates from soil, pulp waste water and rotten wood for cellulase producing potentials
Pulp waste water, soil from agricultural waste dump site and rotten wood were investigated for the presence of fungi with cellulolysic ability. Fungi Isolates obtained from the samples were identified based on cultural and morphological characteristics. Seven species of fungi namely, Aspergillus ni...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Joint Coordination Centre of the World Bank assisted National Agricultural Research Programme (NARP)
2019-07-01
|
| Series: | Journal of Applied Sciences and Environmental Management |
| Subjects: | |
| Online Access: | https://www.ajol.info/index.php/jasem/article/view/188261 |
| _version_ | 1827297121813921792 |
|---|---|
| author | T.E. Effiong M.S. Abdulsalami N.E. Egbe V Bakare |
| author_facet | T.E. Effiong M.S. Abdulsalami N.E. Egbe V Bakare |
| author_sort | T.E. Effiong |
| collection | DOAJ |
| description |
Pulp waste water, soil from agricultural waste dump site and rotten wood were investigated for the presence of fungi with cellulolysic ability. Fungi Isolates obtained from the samples were identified based on cultural and morphological characteristics. Seven species of fungi namely, Aspergillus niger, Rhizopus oryzea and Fusarium from soil, Penicillium notatum, Mucor resmosus and Aureobasidium sp from rotten wood, Trichoderma citrinoviride, Fusarium salani, A niger and P notatum from pulp waste water were isolated. Among the seven fungi species , A. niger, F. salani, and P. notatum recorded the highest frequency of occurrence, (2), while T. citrinoviride, R. oryzea, Aureobassidium sp recorded frequency occurrence of one (1)each and the cellulytic activity was determined by the ratio of zone of clearing and colony diameter. The difference in zone of clearing produced by the fungi isolates (A. niger, T. citrinoviride, F. salani), on CMC containing plate were not significantly different at (p˂ 0.05). Highest cellulase activity ratio was exhibited by T. citrinoviride (1.39), followed by A. niger from soil (1.30).This result highlights the potential of T citrinoviride as strain for industrial production of cellulolysic enzyme.
Keywords: Cellulase, Enzymes, Aspergillus sp., A. niger
|
| first_indexed | 2024-04-24T14:50:07Z |
| format | Article |
| id | doaj.art-46a920dcf9ae44bbaa7c74e5e2a73bf5 |
| institution | Directory Open Access Journal |
| issn | 2659-1502 2659-1499 |
| language | English |
| last_indexed | 2024-04-24T14:50:07Z |
| publishDate | 2019-07-01 |
| publisher | Joint Coordination Centre of the World Bank assisted National Agricultural Research Programme (NARP) |
| record_format | Article |
| series | Journal of Applied Sciences and Environmental Management |
| spelling | doaj.art-46a920dcf9ae44bbaa7c74e5e2a73bf52024-04-02T19:50:32ZengJoint Coordination Centre of the World Bank assisted National Agricultural Research Programme (NARP)Journal of Applied Sciences and Environmental Management2659-15022659-14992019-07-0123610.4314/jasem.v23i6.8Screening of fungi isolates from soil, pulp waste water and rotten wood for cellulase producing potentialsT.E. EffiongM.S. AbdulsalamiN.E. EgbeV Bakare Pulp waste water, soil from agricultural waste dump site and rotten wood were investigated for the presence of fungi with cellulolysic ability. Fungi Isolates obtained from the samples were identified based on cultural and morphological characteristics. Seven species of fungi namely, Aspergillus niger, Rhizopus oryzea and Fusarium from soil, Penicillium notatum, Mucor resmosus and Aureobasidium sp from rotten wood, Trichoderma citrinoviride, Fusarium salani, A niger and P notatum from pulp waste water were isolated. Among the seven fungi species , A. niger, F. salani, and P. notatum recorded the highest frequency of occurrence, (2), while T. citrinoviride, R. oryzea, Aureobassidium sp recorded frequency occurrence of one (1)each and the cellulytic activity was determined by the ratio of zone of clearing and colony diameter. The difference in zone of clearing produced by the fungi isolates (A. niger, T. citrinoviride, F. salani), on CMC containing plate were not significantly different at (p˂ 0.05). Highest cellulase activity ratio was exhibited by T. citrinoviride (1.39), followed by A. niger from soil (1.30).This result highlights the potential of T citrinoviride as strain for industrial production of cellulolysic enzyme. Keywords: Cellulase, Enzymes, Aspergillus sp., A. niger https://www.ajol.info/index.php/jasem/article/view/188261CellulaseEnzymesAspergillus sp.A. niger |
| spellingShingle | T.E. Effiong M.S. Abdulsalami N.E. Egbe V Bakare Screening of fungi isolates from soil, pulp waste water and rotten wood for cellulase producing potentials Journal of Applied Sciences and Environmental Management Cellulase Enzymes Aspergillus sp. A. niger |
| title | Screening of fungi isolates from soil, pulp waste water and rotten wood for cellulase producing potentials |
| title_full | Screening of fungi isolates from soil, pulp waste water and rotten wood for cellulase producing potentials |
| title_fullStr | Screening of fungi isolates from soil, pulp waste water and rotten wood for cellulase producing potentials |
| title_full_unstemmed | Screening of fungi isolates from soil, pulp waste water and rotten wood for cellulase producing potentials |
| title_short | Screening of fungi isolates from soil, pulp waste water and rotten wood for cellulase producing potentials |
| title_sort | screening of fungi isolates from soil pulp waste water and rotten wood for cellulase producing potentials |
| topic | Cellulase Enzymes Aspergillus sp. A. niger |
| url | https://www.ajol.info/index.php/jasem/article/view/188261 |
| work_keys_str_mv | AT teeffiong screeningoffungiisolatesfromsoilpulpwastewaterandrottenwoodforcellulaseproducingpotentials AT msabdulsalami screeningoffungiisolatesfromsoilpulpwastewaterandrottenwoodforcellulaseproducingpotentials AT neegbe screeningoffungiisolatesfromsoilpulpwastewaterandrottenwoodforcellulaseproducingpotentials AT vbakare screeningoffungiisolatesfromsoilpulpwastewaterandrottenwoodforcellulaseproducingpotentials |