The lower airways microbiota and antimicrobial peptides indicate dysbiosis in sarcoidosis
Abstract Background The role of the pulmonary microbiome in sarcoidosis is unknown. The objectives of this study were the following: (1) examine whether the pulmonary fungal and bacterial microbiota differed in patients with sarcoidosis compared with controls; (2) examine whether there was an associ...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2022-10-01
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| Series: | Microbiome |
| Online Access: | https://doi.org/10.1186/s40168-022-01362-4 |
| _version_ | 1798028597805449216 |
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| author | Kristel S. Knudsen Sverre Lehmann Rune Nielsen Solveig Tangedal Andreu Paytuvi-Gallart Walter Sanseverino Einar M. H. Martinsen Pieter S. Hiemstra Tomas M. Eagan |
| author_facet | Kristel S. Knudsen Sverre Lehmann Rune Nielsen Solveig Tangedal Andreu Paytuvi-Gallart Walter Sanseverino Einar M. H. Martinsen Pieter S. Hiemstra Tomas M. Eagan |
| author_sort | Kristel S. Knudsen |
| collection | DOAJ |
| description | Abstract Background The role of the pulmonary microbiome in sarcoidosis is unknown. The objectives of this study were the following: (1) examine whether the pulmonary fungal and bacterial microbiota differed in patients with sarcoidosis compared with controls; (2) examine whether there was an association between the microbiota and levels of the antimicrobial peptides (AMPs) in protected bronchoalveolar lavage (PBAL). Methods Thirty-five sarcoidosis patients and 35 healthy controls underwent bronchoscopy and were sampled with oral wash (OW), protected BAL (PBAL), and left protected sterile brushes (LPSB). The fungal ITS1 region and the V3V4 region of the bacterial 16S rRNA gene were sequenced. Bioinformatic analyses were performed with QIIME 2. The AMPs secretory leucocyte protease inhibitor (SLPI) and human beta defensins 1 and 2 (hBD-1 and hBD-2), were measured in PBAL by enzyme-linked immunosorbent assay (ELISA). Results Aspergillus dominated the PBAL samples in sarcoidosis. Differences in bacterial taxonomy were minor. There was no significant difference in fungal alpha diversity between sarcoidosis and controls, but the bacterial alpha diversity in sarcoidosis was significantly lower in OW (p = 0.047) and PBAL (p = 0.03) compared with controls. The beta diversity for sarcoidosis compared with controls differed for both fungi and bacteria. AMP levels were significantly lower in sarcoidosis compared to controls (SLPI and hBD-1: p < 0.01). No significant correlations were found between alpha diversity and AMPs. Conclusions The pulmonary fungal and bacterial microbiota in sarcoidosis differed from in controls. Lower antimicrobial peptides levels were seen in sarcoidosis, indicating an interaction between the microbiota and the innate immune system. Whether this dysbiosis represents a pathogenic mechanism in sarcoidosis needs to be confirmed in experimental studies. Video Abstract |
| first_indexed | 2024-04-11T19:11:39Z |
| format | Article |
| id | doaj.art-46ac693ef5394def9faf5b77212bd32c |
| institution | Directory Open Access Journal |
| issn | 2049-2618 |
| language | English |
| last_indexed | 2024-04-11T19:11:39Z |
| publishDate | 2022-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbiome |
| spelling | doaj.art-46ac693ef5394def9faf5b77212bd32c2022-12-22T04:07:36ZengBMCMicrobiome2049-26182022-10-0110111110.1186/s40168-022-01362-4The lower airways microbiota and antimicrobial peptides indicate dysbiosis in sarcoidosisKristel S. Knudsen0Sverre Lehmann1Rune Nielsen2Solveig Tangedal3Andreu Paytuvi-Gallart4Walter Sanseverino5Einar M. H. Martinsen6Pieter S. Hiemstra7Tomas M. Eagan8Department of Clinical Science, University of BergenDepartment of Clinical Science, University of BergenDepartment of Clinical Science, University of BergenDepartment of Clinical Science, University of BergenSequentia Biotech SLSequentia Biotech SLDepartment of Clinical Science, University of BergenDepartment of Pulmonology, Leiden University Medical CenterDepartment of Clinical Science, University of BergenAbstract Background The role of the pulmonary microbiome in sarcoidosis is unknown. The objectives of this study were the following: (1) examine whether the pulmonary fungal and bacterial microbiota differed in patients with sarcoidosis compared with controls; (2) examine whether there was an association between the microbiota and levels of the antimicrobial peptides (AMPs) in protected bronchoalveolar lavage (PBAL). Methods Thirty-five sarcoidosis patients and 35 healthy controls underwent bronchoscopy and were sampled with oral wash (OW), protected BAL (PBAL), and left protected sterile brushes (LPSB). The fungal ITS1 region and the V3V4 region of the bacterial 16S rRNA gene were sequenced. Bioinformatic analyses were performed with QIIME 2. The AMPs secretory leucocyte protease inhibitor (SLPI) and human beta defensins 1 and 2 (hBD-1 and hBD-2), were measured in PBAL by enzyme-linked immunosorbent assay (ELISA). Results Aspergillus dominated the PBAL samples in sarcoidosis. Differences in bacterial taxonomy were minor. There was no significant difference in fungal alpha diversity between sarcoidosis and controls, but the bacterial alpha diversity in sarcoidosis was significantly lower in OW (p = 0.047) and PBAL (p = 0.03) compared with controls. The beta diversity for sarcoidosis compared with controls differed for both fungi and bacteria. AMP levels were significantly lower in sarcoidosis compared to controls (SLPI and hBD-1: p < 0.01). No significant correlations were found between alpha diversity and AMPs. Conclusions The pulmonary fungal and bacterial microbiota in sarcoidosis differed from in controls. Lower antimicrobial peptides levels were seen in sarcoidosis, indicating an interaction between the microbiota and the innate immune system. Whether this dysbiosis represents a pathogenic mechanism in sarcoidosis needs to be confirmed in experimental studies. Video Abstracthttps://doi.org/10.1186/s40168-022-01362-4 |
| spellingShingle | Kristel S. Knudsen Sverre Lehmann Rune Nielsen Solveig Tangedal Andreu Paytuvi-Gallart Walter Sanseverino Einar M. H. Martinsen Pieter S. Hiemstra Tomas M. Eagan The lower airways microbiota and antimicrobial peptides indicate dysbiosis in sarcoidosis Microbiome |
| title | The lower airways microbiota and antimicrobial peptides indicate dysbiosis in sarcoidosis |
| title_full | The lower airways microbiota and antimicrobial peptides indicate dysbiosis in sarcoidosis |
| title_fullStr | The lower airways microbiota and antimicrobial peptides indicate dysbiosis in sarcoidosis |
| title_full_unstemmed | The lower airways microbiota and antimicrobial peptides indicate dysbiosis in sarcoidosis |
| title_short | The lower airways microbiota and antimicrobial peptides indicate dysbiosis in sarcoidosis |
| title_sort | lower airways microbiota and antimicrobial peptides indicate dysbiosis in sarcoidosis |
| url | https://doi.org/10.1186/s40168-022-01362-4 |
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