Characterization of the decolorizing activity of azo dyes by Bacillus subtilis azoreductase AzoR1
The product of the Bacillus subtilis gene azoR1 is annotated as a putative azoreductase, production of which isinduced in response to thiol-reactive compounds. Here we report on the decolorization of four azo dyes by azoreductaseactivity. The ability of overexpressed AzoR1 strain ORB7106 to catalyze...
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| Format: | Article |
| Language: | English |
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Prince of Songkla University
2012-11-01
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| Series: | Songklanakarin Journal of Science and Technology (SJST) |
| Subjects: | |
| Online Access: | http://rdo.psu.ac.th/sjstweb/journal/34-5/0475-3395-34-5-509-516.pdf |
| _version_ | 1818108620759367680 |
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| author | Montira Leelakriangsak Sukallaya Borisut |
| author_facet | Montira Leelakriangsak Sukallaya Borisut |
| author_sort | Montira Leelakriangsak |
| collection | DOAJ |
| description | The product of the Bacillus subtilis gene azoR1 is annotated as a putative azoreductase, production of which isinduced in response to thiol-reactive compounds. Here we report on the decolorization of four azo dyes by azoreductaseactivity. The ability of overexpressed AzoR1 strain ORB7106 to catalyze decolorization of azo dyes was investigated on agarplates and in liquid cultures. The decolorization efficacy of a mutant, ORB7106, which has lost negative control of azoR1expression, was significantly better than the parental strain (JH642), providing evidence that the azoR1 gene product isan azoreductase with the ability to decolorize azo dyes. ORB7106 showed 40-98% azo dye decolorization within 48 h. Thebacterium exhibited a remarkable color removal capability over a wide range of Methyl Red concentrations (10-200 mg l-1), pH(5-9) and temperatures (25-45°C). A significant increase in the decolorization activity was observed after induction of AzoR1production. The results suggested that AzoR1 production could be induced by Methyl Red. The decolorizing activity isprimarily associated with the cytosolic fraction and requires NADH as a cofactor. |
| first_indexed | 2024-12-11T02:18:16Z |
| format | Article |
| id | doaj.art-46ac812cee3e4575b4a77f03123dd07a |
| institution | Directory Open Access Journal |
| issn | 0125-3395 |
| language | English |
| last_indexed | 2024-12-11T02:18:16Z |
| publishDate | 2012-11-01 |
| publisher | Prince of Songkla University |
| record_format | Article |
| series | Songklanakarin Journal of Science and Technology (SJST) |
| spelling | doaj.art-46ac812cee3e4575b4a77f03123dd07a2022-12-22T01:24:07ZengPrince of Songkla UniversitySongklanakarin Journal of Science and Technology (SJST)0125-33952012-11-01345509516Characterization of the decolorizing activity of azo dyes by Bacillus subtilis azoreductase AzoR1Montira LeelakriangsakSukallaya BorisutThe product of the Bacillus subtilis gene azoR1 is annotated as a putative azoreductase, production of which isinduced in response to thiol-reactive compounds. Here we report on the decolorization of four azo dyes by azoreductaseactivity. The ability of overexpressed AzoR1 strain ORB7106 to catalyze decolorization of azo dyes was investigated on agarplates and in liquid cultures. The decolorization efficacy of a mutant, ORB7106, which has lost negative control of azoR1expression, was significantly better than the parental strain (JH642), providing evidence that the azoR1 gene product isan azoreductase with the ability to decolorize azo dyes. ORB7106 showed 40-98% azo dye decolorization within 48 h. Thebacterium exhibited a remarkable color removal capability over a wide range of Methyl Red concentrations (10-200 mg l-1), pH(5-9) and temperatures (25-45°C). A significant increase in the decolorization activity was observed after induction of AzoR1production. The results suggested that AzoR1 production could be induced by Methyl Red. The decolorizing activity isprimarily associated with the cytosolic fraction and requires NADH as a cofactor.http://rdo.psu.ac.th/sjstweb/journal/34-5/0475-3395-34-5-509-516.pdfBacillus subtilisAzoR1decolorizationazoreductaseMethyl Red |
| spellingShingle | Montira Leelakriangsak Sukallaya Borisut Characterization of the decolorizing activity of azo dyes by Bacillus subtilis azoreductase AzoR1 Songklanakarin Journal of Science and Technology (SJST) Bacillus subtilis AzoR1 decolorization azoreductase Methyl Red |
| title | Characterization of the decolorizing activity of azo dyes by Bacillus subtilis azoreductase AzoR1 |
| title_full | Characterization of the decolorizing activity of azo dyes by Bacillus subtilis azoreductase AzoR1 |
| title_fullStr | Characterization of the decolorizing activity of azo dyes by Bacillus subtilis azoreductase AzoR1 |
| title_full_unstemmed | Characterization of the decolorizing activity of azo dyes by Bacillus subtilis azoreductase AzoR1 |
| title_short | Characterization of the decolorizing activity of azo dyes by Bacillus subtilis azoreductase AzoR1 |
| title_sort | characterization of the decolorizing activity of azo dyes by bacillus subtilis azoreductase azor1 |
| topic | Bacillus subtilis AzoR1 decolorization azoreductase Methyl Red |
| url | http://rdo.psu.ac.th/sjstweb/journal/34-5/0475-3395-34-5-509-516.pdf |
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