Seed-specific elevation of non-symbiotic hemoglobin <it>AtHb1</it>: beneficial effects and underlying molecular networks in <it>Arabidopsis thaliana</it>
<p>Abstract</p> <p>Background</p> <p>Seed metabolism is dynamically adjusted to oxygen availability. Processes underlying this auto-regulatory mechanism control the metabolic efficiency under changing environmental conditions/stress and thus, are of relevance for biotec...
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| Format: | Article |
| Language: | English |
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BMC
2011-03-01
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| Series: | BMC Plant Biology |
| Online Access: | http://www.biomedcentral.com/1471-2229/11/48 |
| _version_ | 1818471191802806272 |
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| author | Tschiersch Henning Feil Regina Nguyen Thuy H Lunn John E Friedel Svetlana Rolletschek Hardy Thiel Johannes Müller Martin Borisjuk Ljudmilla |
| author_facet | Tschiersch Henning Feil Regina Nguyen Thuy H Lunn John E Friedel Svetlana Rolletschek Hardy Thiel Johannes Müller Martin Borisjuk Ljudmilla |
| author_sort | Tschiersch Henning |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Seed metabolism is dynamically adjusted to oxygen availability. Processes underlying this auto-regulatory mechanism control the metabolic efficiency under changing environmental conditions/stress and thus, are of relevance for biotechnology. Non-symbiotic hemoglobins have been shown to be involved in scavenging of nitric oxide (NO) molecules, which play a key role in oxygen sensing/balancing in plants and animals. Steady state levels of NO are suggested to act as an integrator of energy and carbon metabolism and subsequently, influence energy-demanding growth processes in plants.</p> <p>Results</p> <p>We aimed to manipulate oxygen stress perception in <it>Arabidopsis </it>seeds by overexpression of the non-symbiotic hemoglobin <it>AtHb1 </it>under the control of the seed-specific LeB4 promoter. Seeds of transgenic <it>AtHb1 </it>plants did not accumulate NO under transient hypoxic stress treatment, showed higher respiratory activity and energy status compared to the wild type. Global transcript profiling of seeds/siliques from wild type and transgenic plants under transient hypoxic and standard conditions using Affymetrix ATH1 chips revealed a rearrangement of transcriptional networks by <it>AtHb1 </it>overexpression under non-stress conditions, which included the induction of transcripts related to ABA synthesis and signaling, receptor-like kinase- and MAP kinase-mediated signaling pathways, WRKY transcription factors and ROS metabolism. Overexpression of <it>AtHb1 </it>shifted seed metabolism to an energy-saving mode with the most prominent alterations occurring in cell wall metabolism. In combination with metabolite and physiological measurements, these data demonstrate that <it>AtHb1 </it>overexpression improves oxidative stress tolerance compared to the wild type where a strong transcriptional and metabolic reconfiguration was observed in the hypoxic response.</p> <p>Conclusions</p> <p><it>AtHb1 </it>overexpression mediates a pre-adaptation to hypoxic stress. Under transient stress conditions transgenic seeds were able to keep low levels of endogenous NO and to maintain a high energy status, in contrast to wild type. Higher weight of mature transgenic seeds demonstrated the beneficial effects of seed-specific overexpression of <it>AtHb1</it>.</p> |
| first_indexed | 2024-04-14T03:47:46Z |
| format | Article |
| id | doaj.art-46ac98a5fcd74621a4d47309b1113179 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-04-14T03:47:46Z |
| publishDate | 2011-03-01 |
| publisher | BMC |
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| series | BMC Plant Biology |
| spelling | doaj.art-46ac98a5fcd74621a4d47309b11131792022-12-22T02:14:09ZengBMCBMC Plant Biology1471-22292011-03-011114810.1186/1471-2229-11-48Seed-specific elevation of non-symbiotic hemoglobin <it>AtHb1</it>: beneficial effects and underlying molecular networks in <it>Arabidopsis thaliana</it>Tschiersch HenningFeil ReginaNguyen Thuy HLunn John EFriedel SvetlanaRolletschek HardyThiel JohannesMüller MartinBorisjuk Ljudmilla<p>Abstract</p> <p>Background</p> <p>Seed metabolism is dynamically adjusted to oxygen availability. Processes underlying this auto-regulatory mechanism control the metabolic efficiency under changing environmental conditions/stress and thus, are of relevance for biotechnology. Non-symbiotic hemoglobins have been shown to be involved in scavenging of nitric oxide (NO) molecules, which play a key role in oxygen sensing/balancing in plants and animals. Steady state levels of NO are suggested to act as an integrator of energy and carbon metabolism and subsequently, influence energy-demanding growth processes in plants.</p> <p>Results</p> <p>We aimed to manipulate oxygen stress perception in <it>Arabidopsis </it>seeds by overexpression of the non-symbiotic hemoglobin <it>AtHb1 </it>under the control of the seed-specific LeB4 promoter. Seeds of transgenic <it>AtHb1 </it>plants did not accumulate NO under transient hypoxic stress treatment, showed higher respiratory activity and energy status compared to the wild type. Global transcript profiling of seeds/siliques from wild type and transgenic plants under transient hypoxic and standard conditions using Affymetrix ATH1 chips revealed a rearrangement of transcriptional networks by <it>AtHb1 </it>overexpression under non-stress conditions, which included the induction of transcripts related to ABA synthesis and signaling, receptor-like kinase- and MAP kinase-mediated signaling pathways, WRKY transcription factors and ROS metabolism. Overexpression of <it>AtHb1 </it>shifted seed metabolism to an energy-saving mode with the most prominent alterations occurring in cell wall metabolism. In combination with metabolite and physiological measurements, these data demonstrate that <it>AtHb1 </it>overexpression improves oxidative stress tolerance compared to the wild type where a strong transcriptional and metabolic reconfiguration was observed in the hypoxic response.</p> <p>Conclusions</p> <p><it>AtHb1 </it>overexpression mediates a pre-adaptation to hypoxic stress. Under transient stress conditions transgenic seeds were able to keep low levels of endogenous NO and to maintain a high energy status, in contrast to wild type. Higher weight of mature transgenic seeds demonstrated the beneficial effects of seed-specific overexpression of <it>AtHb1</it>.</p>http://www.biomedcentral.com/1471-2229/11/48 |
| spellingShingle | Tschiersch Henning Feil Regina Nguyen Thuy H Lunn John E Friedel Svetlana Rolletschek Hardy Thiel Johannes Müller Martin Borisjuk Ljudmilla Seed-specific elevation of non-symbiotic hemoglobin <it>AtHb1</it>: beneficial effects and underlying molecular networks in <it>Arabidopsis thaliana</it> BMC Plant Biology |
| title | Seed-specific elevation of non-symbiotic hemoglobin <it>AtHb1</it>: beneficial effects and underlying molecular networks in <it>Arabidopsis thaliana</it> |
| title_full | Seed-specific elevation of non-symbiotic hemoglobin <it>AtHb1</it>: beneficial effects and underlying molecular networks in <it>Arabidopsis thaliana</it> |
| title_fullStr | Seed-specific elevation of non-symbiotic hemoglobin <it>AtHb1</it>: beneficial effects and underlying molecular networks in <it>Arabidopsis thaliana</it> |
| title_full_unstemmed | Seed-specific elevation of non-symbiotic hemoglobin <it>AtHb1</it>: beneficial effects and underlying molecular networks in <it>Arabidopsis thaliana</it> |
| title_short | Seed-specific elevation of non-symbiotic hemoglobin <it>AtHb1</it>: beneficial effects and underlying molecular networks in <it>Arabidopsis thaliana</it> |
| title_sort | seed specific elevation of non symbiotic hemoglobin it athb1 it beneficial effects and underlying molecular networks in it arabidopsis thaliana it |
| url | http://www.biomedcentral.com/1471-2229/11/48 |
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